The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey

Yıl 2018, Cilt: 29 Sayı: 2, 104 - 110, 30.12.2018

Turhan Turan Hakan Işıdan

https://doi.org/10.35864/evmd.513442

Cited By: 3

Öz

A total of 127 stool samples (rectal swab) from diarrheic calves, up to one mouth of age, were collected

during one year period (2014-16) from three cities (Sivas, Malatya and Elazığ) located central Turkey. 432 bp partial

sequence of Nsp1ab gene were amplified. The PCR amplicons were purified and sequenced and deposited to GenBank.

Sequence alignment and phylogenetic analysis being based on partial nucleotide sequences of Nsp1ab gene was constructed.

As a result of RT-PCR study, we found that the 3.15% of fecal samples (4/127) were positive for diarrhea calves

from Turkey. The neighbor-joinning tree of partial sequence of Nsp1ab gene (ORF1a) indicated that strains were substituted

under three distinct lineage. None of our strains belonged to the Neuro1 lineage (lineage 3) of Bovine astrovirus

which was isolated from bovine with neurological symptoms. On the other hand, while BAstVHT1-TUR strain was

substituted under lineage 1a, the other novel starts were lineage 2. While the identity of analyzed sequences varies from

51.1 to 100%, novel strains were calculated between 75.8 to 100 % each other. As a result, we report the first detection

and phylogenetic analysis of Bovine astrovirus from Turkey.

Anahtar Kelimeler

Bovine astrovirus, calf, phylogenetic analysis, Turkey

Kaynakça

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