Year 2018, Volume 29, Issue 2, Pages 175 - 180 2018-12-30

Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı

Aylin Pehlivan [1] , Enes Atmaca [2] , Abdurrahman Aksoy [3]

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Son yıllarda, pratik ve etik nedenlerle, ekotoksikolojide in vitro yöntemlere olan ilgi artmıştır. Sitotoksisite çalışmalarında hücre kültürü uygulamaları, in vivo çalışmalardan elde edilen sonuçlara uyumluluğu ve hücresel düzeyde inceleme olanağı gibi avantajlarıyla, diğer in vitro yöntemlere göre öne çıkmıştır. Balık hücre kültürleriyle yapılan çalışmalar, kimyasal maddelerin toksik ve çevresel etkilerinin değerlendirilmesine imkan sağlamaktadır. Ekotoksikolojide, kimyasal maddelerin toksik ve çevresel etkilerinin değerlendirilmesinde RTG-2, RTL-W1, PLHC-1 ve EPC gibi çeşitli balık hücre hatları sıklıkla tercih edilmektedir. Bu derlemede, balık hücre hatlarının ekotoksikoloji çalışmalarında kullanımları ve dikkat edilmesi gereken bazı hususlar ele alınmıştır.
Çevresel kirleticiler, ekotoksikoloji, balık hücre hattı
  • 1. Acker LA, McMahan JR, Gawel JE, (2005). The effect of heavy metal pollution in aquatic environments on metallothionein production in Mytilus sp. Proceedings of the 2005 Puget Sound Georgia Basin Research Conference.
  • 2. Ackermann GE, Brombacher E, Fent K, (2002). Development of a fish reporter gene system for the assessment of estrogenic compounds and sewage treatment plant effluents. Environ Toxicol Chem. 21, 1864-1875.
  • 3. Araujo CSA, Marques SAF, Carrondo MJT, Gonçalves LMD, (2000). In vitro response of the brown bullhead catfish (BB) and rainbow trout (RTG-2) cell lines to benzo(a) pyrene. Sci Total Environ. 247, 127-135.
  • 4. ATCC, (2018). Erişim adresi: https://www.lgcstandardsatcc. org/en.aspx , Erişim tarihi: 09.11.2018.
  • 5. Babin MM, Tarazona JV, (2005). In vitro toxicity of selected pesticides on RTG-2 and RTL-W1 fish cell lines. Environ Pollut. 135, 267-274.
  • 6. Babin M, Boleas S, Tarazona JV, (2005). In vitro toxicity of antimicrobials on RTG-2 and RTL-W1 fish cell lines. Environ Toxicol Pharmacol. 20, 125–134.
  • 7. Baby J, Raj JS, Biby ET, Sankarganesh P, Jeevitha MV, Ajisha SU, Rajan SS, (2010). Toxic effect of heavy metals on aquatic environment. Int J Biol Chem Sci. 4(4), 939- 952.
  • 8. Behrens A, Schirmer K, Bols NC, Segner H, (2001). Polycyclic aromatic hydrocarbons as inducers of cytochrome P4501A enzyme activity in the rainbow trout liver cell line, RTL-W1 and in primary cultures of rainbow trout hepatocytes. Environ Toxicol Chem. 20(3), 632-643.
  • 9. Birnbaum LS, Tuomisto J, (2000). Non-carcinogenic effects of TCDD in animals. Food Addit Contam. 17(4), 275-288.
  • 10. Bols NC, Dayeh VR, Lee LEJ, Schirmer K, (2005). Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology. Mommsen TP, Moon TW. eds. Biochemistry and molecular biology of fishes. Elsevier B.V. p.43-84.
  • 11. Bols NC, Pham PH, Dayeh VR, Lee LEJ, (2017). Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout. In Vitro Cell Dev Biol. 53, 383-405.
  • 12. Brack W, Schirmer K, Kind T, Schrader S, Schüürmann G, (2002). Effect-directed fractionation and identification of cytochrome P4501A-inducing halogenated aromatic hydrocarbons in a contaminated sediment. Environ Toxicol Chem. 21(12), 2654–2662.
  • 13. Caminada D, Escher C, Fent K, (2006). Cytotoxicity of pharmaceuticals found in aquatic systems: Comparison of PLHC-1 and RTG-2 fish cell lines. Aquat Toxicol. 79, 114-123.
  • 14. Castano A, Bols N, Braunbeck T, Dierickx P, Halder M, Isomaa B, Kawahara K, Lee LEJ, Mothersill C, Pärt P, Repetto G, Sintes JR, Rufli H, Smith R, Wood C, Segner H, (2003). The use of fish cells in ecotoxicology. ATLA. 31, 317-351.
  • 15. Celander M, Hahn ME, Stegeman JJ, (1996). Cytochromes P450 (CYP) in the Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1): Dose and time-dependent glucocorticoid potentiation of CYP1A induction without induction of CYP3A. Arch Biochem Biophys. 329(1), 113- 122.
  • 16. Coyle P, Philcox JC, Carey LC, Rofe AM, (2002). Metallothionein: The multipurpose protein. Cell Mol Life Sci. 59, 627-647.
  • 17. Dayeh VR, Schirmer K, Bols NC, (2002). Applying wholewater samples directly to fish cell cultures in order to evaluate the toxicity of industrial effluent. Water Res. 36, 3727-3738.
  • 18. Fent K, (2001). Fish cell lines as versatile tools in ecotoxicology: assessment of cytotoxicity, cytochrome P4501A induction potential and estrogenic activity of chemicals and environmental samples. Toxicol In Vitro. 15, 477-488.
  • 19. Fent K, (2007). Permanent fish cell cultures as important tools in ecotoxicology. ALTEX. 24(8), 26-28.
  • 20. Fent K, Batscher R, (2000). Cytochrome P4501A induction potencies of polycyclic aromatic hydrocarbons in a fish hepatoma cell line: demonstration of additive interactions. Environ Toxicol Chem. 19(8), 2047–2058.
  • 21. Fent K, Hunn J, (1996). Cytotoxicity of organic environmental chemicals to fish liver cells (PLHC-1). Mar Environ Res. 42(14), 377-382.
  • 22. IARC, (2010). Benzo[a]pyrene. https://monographs.iarc. fr/wp-content/uploads/2018/06/mono100F-14.pdf ,Erişim tarihi: 30.11.2018.
  • 23. Kammann U, Bunke M, Steinhart H, Theobald N, (2001). A permanent fish cell line (EPC) for genotoxicity testing of marine sediments with the comet assay. Mutat Res Genet Toxicol Environ Mutagen. 498(1-2), 67-77.
  • 24. Kling P, Erkell LJ, Kille P, Olsson PE, (1996). Metallothionein induction in rainbow trout gonadal (RTG-2) cells during free radical exposure. Mar Environ Res. 42(1-4), 33-36.
  • 25. Lakra WS, Raja Swaminathan T, Joy KP, (2011). Development, characterization, conservation and storage of fish cell lines: a review. Fish Physiol Biochem. 37, 1-20.
  • 26. Lange M, Gebauer W, Markl J, Nagel R, (1995). Comparison of testing acute toxicity on embryo of zebrafish, Brachydanio rerio and RTG-2 cytotoxicity as possible alternatives to the acute fish test. Chemosphere. 30(11), 2087-2102.
  • 27. Lee LEJ, Clemons JH, Bechtel DG, Caldwell SJ, Han KB, Pasitschniak-Arts M, Mosser DD, Bols NC, (1993). Development and characterization of a rainbow trout liver cell line expressing cytochrome P450-dependent monooxygenase activity. Cell Biol Toxicol. 9(3), 279-294.
  • 28. Lee LEJ, Dayeh VR, Schirmer K, Bols NC, (2009). Applications and potential uses of fish gill cell lines: examples with RTgill-W1. In Vitro Cell Dev Biol. DOI:10.1007/ s11626-008-9173-2.
  • 29. Li H, Zhang S, (2001). In vitro cytotoxicity of the organophosphorus pesticide parathion to FG-9307 cells. Toxicol In Vitro. 15, 643-647.
  • 30. Llorente MT, Parra JM, Sanchez-Fortun S, Castano A, (2012). Cytotoxicity and genotoxicity of sewage treatment plant effluents in rainbow trout cells (RTG-2). Water Res. 46, 6351-58
  • 31. Maracine M, Segner H, (1998). Cytotoxicity of metals in isolated fish cells: Importance of the cellular glutathione status. Comp Biochem Physiol A.120, 83–88.
  • 32. Nehls S, Segner H, (2001). Detection of DNA damage in two cell lines from rainbow trout, RTG-2 and RTL-W1, using the Comet assay. Environ Toxicol. 16, 321-329.
  • 33. Nehls S, Segner H, (2005). Comet assay with the fish cell line rainbow trout gonad-2 for in vitro genotoxicity testing of xenobiotics and surface waters. Environ Toxicol Chem. (24)8, 2078–2087.
  • 34. Parra J.M, Sanchez-Fortun S, Castano A, (2012). Assessment of genotoxic effects induced by selected pesticides on RTG-2 fish cells by means of a modified fast micromethod assay. Environ Toxicol. 27, 238–243.
  • 35. Rachlin JW, Perlmutter A, (1968). Fish cells in culture for study of aquatic toxicants. Water Res. 2(6), 409-414.
  • 36. Roux F, (2015). Fish cell lines and their potential uses in ecotoxicology: from cytotoxicity studies and mixture assessment to a co-culture model and mechanistic analyses. Master Thesis, Department of Biological and Environmental Sciences University of Gothenburg, Gothenburg.
  • 37. Sanchez P, Llorente M.T, Castano A, (2000). Flow cytometric detection of micronuclei and cell cycle alterations in fish-derived cells after exposure to three model genotoxic agents: mitomycin C, vincristine sulfate and benzo(a) pyrene. Mutat Res. 465, 113–122.
  • 38. Schirmer K, Dixon DG, Greenberg BM, Bols NC, (1998). Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill. Toxicology. 127, 129–141.
  • 39. Schirmer K, Chan AGJ, Bols NC, (2000). Transitory metabolic disruption and cytotoxicity elicited by benzo[a]pyrene in two cell lines from rainbow trout liver. J Biochem Mol Toxicol. 14(5), 262-276.
  • 40. Schirmer K, (2006). Proposal to improve vertebrate cell cultures to establish them as substitutes for the regulatory testing of chemicals and effluents using fish. Toxicology. 224, 163-183.
  • 41. Schlenk D, Rice CD, (1998). Effect of zinc and cadmium treatment on hydrogen peroxide-induced mortality and expression of glutathione and metallothionein in a teleost hepatoma cell line. Aquat Toxicol. 43, 121–129.
  • 42. Tan F, Wang M, Wang W, Lu Y, (2008). Comparative evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro. Toxicol In Vitro. 22, 164-170.
  • 43. Thibaut R, Porte C, (2008). Effects of fibrates, anti-inflammatory drugs and antidepressants in the fish hepatoma cell line PLHC-1: Cytotoxicity and interactions with cytochrome P4501A. Toxicol in Vitro. 22, 1128–1135.
  • 44. Wolf K, Quimby MC, (1962). Established eurythermic line of fish cells in vitro. Science. 135(3508), 1065-10.
Primary Language tr
Journal Section Issue
Authors

Author: Aylin Pehlivan (Primary Author)
Institution: ONDOKUZ MAYIS ÜNİVERSİTESİ

Author: Enes Atmaca
Institution: ONDOKUZ MAYIS ÜNİVERSİTESİ

Author: Abdurrahman Aksoy
Institution: ONDOKUZ MAYIS ÜNİVERSİTESİ

Bibtex @review { evmd513576, journal = {Etlik Veteriner Mikrobiyoloji Dergisi}, issn = {1016-3573}, address = {Veteriner Kontrol Merkez Araştırma Enstitüsü}, year = {2018}, volume = {29}, pages = {175 - 180}, doi = {}, title = {Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı}, key = {cite}, author = {Pehlivan, Aylin and Atmaca, Enes and Aksoy, Abdurrahman} }
APA Pehlivan, A , Atmaca, E , Aksoy, A . (2018). Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı. Etlik Veteriner Mikrobiyoloji Dergisi, 29 (2), 175-180. Retrieved from http://dergipark.org.tr/evmd/issue/42562/513576
MLA Pehlivan, A , Atmaca, E , Aksoy, A . "Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı". Etlik Veteriner Mikrobiyoloji Dergisi 29 (2018): 175-180 <http://dergipark.org.tr/evmd/issue/42562/513576>
Chicago Pehlivan, A , Atmaca, E , Aksoy, A . "Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı". Etlik Veteriner Mikrobiyoloji Dergisi 29 (2018): 175-180
RIS TY - JOUR T1 - Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı AU - Aylin Pehlivan , Enes Atmaca , Abdurrahman Aksoy Y1 - 2018 PY - 2018 N1 - DO - T2 - Etlik Veteriner Mikrobiyoloji Dergisi JF - Journal JO - JOR SP - 175 EP - 180 VL - 29 IS - 2 SN - 1016-3573- M3 - UR - Y2 - 2018 ER -
EndNote %0 Journal of Etlik Veterinary Microbiology Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı %A Aylin Pehlivan , Enes Atmaca , Abdurrahman Aksoy %T Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı %D 2018 %J Etlik Veteriner Mikrobiyoloji Dergisi %P 1016-3573- %V 29 %N 2 %R %U
ISNAD Pehlivan, Aylin , Atmaca, Enes , Aksoy, Abdurrahman . "Ekotoksikoloji Alanında Balık Hücre Hatlarının Kullanımı". Etlik Veteriner Mikrobiyoloji Dergisi 29 / 2 (December 2019): 175-180.