Year 2019, Volume 30, Issue 1, Pages 51 - 57 2019-06-30

Investigating the critical formulation temperatures of Brucella abortus S-19 vaccine with different liyoprotectants prior to liyophilisation
Brusella abortus S-19 aşısının liyofilizasyon öncesi farklı liyoprotektanlar ile kritik formülasyon sıcaklıklarının araştırılması

Mustafa Sencer Karagül [1] , Buket Eroğlu [2]

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The aim of this study is to determine the critical temperatures of formulations composed of B.abortus S19 vaccine strain and liyoprotectants. It also attempts to obtain the necessary information that may be beneficial for the optimisation of lyophilisation process by the help of comparative results among formulations. Thermal analysis of 9 different formulations consisting of S19 strain and each of 3 lyprotectants (mannitol, sucrose, trehalose) at 3 different concentrations (5%, 10%,15%) was carried out by freze drying microscopy (FDM), differential thermal analysis (DTA), and electrical impedance (Zsin) analysis. Correlations between liyoprotectant choice and liyoprotectant concentration were investigated by the help of critical formulation temperatures obtained through thermal analysis and screening instruments. Significant differences in the critical temperature of vaccine with liyoprotectant formulation were observed when a different liyoprotectant was used. However, differences that require the redesign of the whole drying process were not observed at the different concentrations of the same liyoprotectant. For this reason,  in the quide of a baseline recipe, evaluation of the liyophilisation process carried out through increasing the amount of liyoprotectant can be recommended to increase stability and performance.

Bu çalışmada, Brusella (B.)abortus S19 aşı şuşu ile liyoprotektan karışımlarının termal analizi yapılarak kritik formülasyon sıcaklıklarının belirlenmesi, karşılaştırmalı sonuçlar ile liyofilizasyon optimizasyonu için istifade edilebilecek bilgilere ulaşılması amaçlanmıştır. S19 aşısı ile 3 farklı liyoprotektan (mannitol, sukroz, trehaloz) 3 farklı konsantrasyonda (%5, %10, %15) kullanılarak 9 farklı formülasyonun analizi termal görüntüleme cihazı olan dondurarak kurutma mikroskopu (FDM) ve Diferansiyel Termal Analiz (DTA) ile elektriksel direnç(Zsin) analizini sağlayan termal analiz cihazı ile yapılmıştır. Termal analiz ve görüntüleme cihazların kullanımıyla elde edilen kritik formülasyon sıcaklıkları ile liyoprotektan tercihi ve konsantrasyonu arasındaki ilişki araştırılmıştır.  Aşı yarı mamulü ile liyoprotektan karışımları için formülasyonların kritik sıcaklıklardaki kayda değer farklar, farklı liyoprotektan kullanıldığında gözlenmiştir. Her liyoprotektanın farklı konsantrasyonları için ise kurutma prosesinin yeni baştan dizayn edilmesini gerektiren farklar tespit edilmemiştir. Bu açıdangelecek çalışmalar için oluşturulmuş temel bir reçete üzerinden protektan madde konsantrasyonunun arttırılarak yapılacak liyofilizasyon proseslerinin yürütülmesi verim ve stabiliteyi artırabilecektir.

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Primary Language tr
Journal Section Original Article
Authors

Orcid: 0000-0001-7215-5229
Author: Mustafa Sencer Karagül (Primary Author)
Institution: KOCAELİ ÜNİVERSİTESİ, KARTEPE ATÇILIK MESLEK YÜKSEKOKULU
Country: Turkey


Orcid: 0000-0001-7748-7898
Author: Buket Eroğlu
Institution: Pendik Veteriner Kontrol Enstitüsü
Country: Turkey


Dates

Publication Date: June 30, 2019

APA Karagül, M , Eroğlu, B . (2019). Brusella abortus S-19 aşısının liyofilizasyon öncesi farklı liyoprotektanlar ile kritik formülasyon sıcaklıklarının araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 30 (1), 51-57. Retrieved from http://dergipark.org.tr/evmd/issue/46765/560109