Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascarididae) larvae infecting Merlangius merlangus euxinus (Linnaeus, 1758) from the Turkish Black Sea coast based on mitochondrial small subunit ribosomal RNA gene analysis

Yıl 2019, Cilt: 30 Sayı: 1, 64 - 69, 30.06.2019

Gökmen Zafer Pekmezci

https://doi.org/10.35864/evmd.562427

Cited By: 4

Öz

The taxonomy of Hysterothylacium
genus remains incomplete and unclear in Turkish waters. In the present study, H. adumcum larvae were morphologically identified
from Merlangius merlangus euxinus in
the Black Sea, Turkey.  The
nuclear ribosomal ITS regions (ITS-1, 5.8S subunit, ITS-2) and the small
subunit of the mitochondrial ribosomal RNA (rrnS) gene of H. aduncum were amplified and sequenced. The BLAST analysis indicated
that present ITS sequences of H. aduncum
were identical to that of the reference sequence of H. aduncum (accession no JX413596) recorded previously from the Black
Sea, Turkey. The present rrnS gene of H.
aduncum from the Black Sea, Turkey (MK886768) showed 97.94 to 99.56%
identity with isolates of H. aduncum
from the Mediterranean Sea (MF000685-MF000691) and the Chinese waters
(MF140344). Moreover, pairwise comparison between the rrnS sequences of the H. aduncum from the Black Sea, Turkey (MK886768)
and others H. aduncum isolates from
the Mediterranean Sea (MF000685-MF000691), the Chinese waters (MF140344) showed
differences ranged from 0.2 and 1.7%.  With
the present study, H. aduncum from the Black Sea was characterized for
the first time by sequencing of the mitochondrial rrnS gene.

Anahtar Kelimeler

Hysterothylacium aduncum, Molecular characterization, mitochondrial rrnS gene, Black Sea, Turkey

Küçük alt ünite ribozomal RNA gen analizine dayanarak Türkiye’nin Karadeniz kıyılarından Merlangius merlangus euxinus’u (Linnaeus, 1758) enfekte eden Hysterothylacium aduncum (Nematoda: Raphidascarididae) larvasının moleküler karakterizasyonu

Öz

Türkiye
sularında Hysterothylacium cinsinin
taksonomisi eksik ve belirsizdir. Bu araştırmada Türkiye’nin Karadeniz
kıyılarında avlanan Merlangius merlangus
euxinus’da H. aduncum larvaları
morfolojik olarak teşhis edildi. H.
aduncum’un nükleer ribozomal ITS gen bölgesi (ITS-1, 5.8S subunit, ITS-2) ile
küçük alt ünite mitokondriyal ribozomal RNA (rrnS) gen bölgesi çoğaltıldı ve
sekanslandı. BLAST analizleri H. aduncum’un mevcut ITS dizilerilerinin
daha önce Türkiye Karadeniz’den kayıt edilen H. aduncum’un referans dizisiyle (erişim numarası JX413596) aynı
olduğunu gösterdi. Türkiye Karadeniz’den H.
aduncum türünün rrnS geni (MK886768), H.
aduncum'un Akdeniz (MF000685-MF000691) ile Çin sularındaki (MF140344) izolatları
ile %97.94-99.56 arasında benzerlik gösterdi. Bununla birlikte, Türkiye Karadeniz’den
elde edilen H. aduncum’un rrnS dizisi
ile H. aduncum'un Akdeniz
(MF000685-MF000691) ile Çin sularındaki (MF140344) diğer izolatlarının ikili
karşılaştırmasında %0,2 ile %1,7 arasında farklılık gözlendi. Bu çalışma ile ilk
kez Karadeniz'den H. aduncum’un mitokondriyal
rrnS geni moleküler olarak karakterize edildi.

Anahtar Kelimeler

Hysterothylacium aduncum, Moleküler Karakterizasyon, Mitokondriyal rrnS geni, Karadeniz, Türkiye

Kaynakça

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