Investigation of the IL-1 β Genotype and its Levels on Patients Treated with Osteointegrated Dental Implants

Yıl 2019, Cilt: 9 Sayı: 1, 11 - 15, 30.04.2019

Ferhat Dizen Uzay Görmüş Arzu Ergen Meral Ünür Elif Sinem İplik Bedia Çakmakoğlu Turgay İsbir

https://doi.org/10.26650/experimed.2019.19002

Öz

DOI:
10.26650/experimed.2019.19002

Objective: In our study, we aimed to investigate the relationship between the Interleukin-1β (IL-1β) +3953 gene polymorphism, the levels of this interleukin and smoking in osteointegrated dental implant losses.

Materials and Methods: 56 patients with
implants and 238 healthy control subjects were included in this study. PCR and
RFLP methods was used to determine the IL-1β +3953 gene polymorphisms. Serum IL-1β levels were measured using ELISA.

Results: The IL-1β  +3953   CC, TT and CT genotype frequencies for the
controls and study cases were 61.2%, 
10.1% ,43.6%, and 48.2%, 8.9% and 42.9%, respectively. IL-1β  +3953 T allele carriers
increased more in the patient group (51.8%) compared  to the control group (38.8%). These cases
also  had an increased plaque index,
bleeding index, pocket deepness and serum IL-1β levels, but none of them were statistically significant
(p>0.05). Implant losses were more frequent in C allele carriers than in T
allele carriers, but this was not statistically significant (p>0.05) Three
of the five patients that experienced implant loss and  smoked, lost the implant earlier and had a
higher average plaque and bleeding index.

Conclusion: Based on our results, we can suggest
that being an IL-1β positive
genotype and a smoker can have an effect on implant losses. The study group
should be enlarged to comment with statistically significant results.

Cite this article as: Dizen F, Görmüş U,
Ergen A, Ünür M, İplik ES, Çakmakoğlu B, et al. Investigation of the IL-1 β Genotype and its Levels on Patients Treated with Osteointegrated
Dental Implants. Experimed 2019; 9(1): 11-5.

Anahtar Kelimeler

IL-1β +3953, Implant, Interleukin, polymorphism

Osteointegre Dental İmplant Tedavisi Gören Hastalarda IL-1 β Genotipi ve Seviyelerinin İncelenmesi

Öz

DOI:
10.26650/experimed.2019.19002

Amaç: Çalışmamızda osteointegre dental
implant kayıplarında interlökin-1β (IL-1β) +3953 gen
polimorfizminin serum interlökin düzeyleri ve sigara içimiyle ilişkisini araştırmayı
amaçladık.

Yöntemler: İmplant yapılan 56 olgu ve 238
sağlıklı kontrol öalışmaya dahil edilmiştir. IL-1β +3953 polimorfizmi, polimeraz zincirleme reaksiyonu ve restriksiyon
fragmanı uzunluk polimorfizmi yöntemleri kullanılarak tayin edilmiştir. Serum örneklerinde
IL-1β seviyesi ELISA yöntemi ile
saptanmıştır.

Bulgular: IL-1β  +3953 polimorfizmi; CC, TT
ve CT genotip frekansları kontrol ve hasta grubu için sırasıyla 61.2%,  10.1% , 43.6%, ve 48.2%, 8.9% ve 42.9% olarak
bulunmuştur. IL-1β +3953 T
alleli taşıyanların hastalarda (51.8%) kontrol grubuna (38.8%) göre daha yüksek
bulunmuştur ve bu kişilerde plak indeksi, kanama indeksi, cep derinliği ve
serum IL-1β düzeylerinin
arttığı, sigara içme ve implant kaybı oranının yükseldiği fakat istatistiksel
anlamlılığa ulaşmadığı tespit edilmiştir (p>0,05). İmplant kaybında, C
alleli taşıyanlarda T alleli taşıyanlara göre daha anlamlı bulunmasına rağmen
istatistiksel olarak anlamlı olmadığı görülmüştür (p>0,05).  İmplant kaybı olan beş hastanın üçünde IL-1β pozitif olduğu, hepsinin sigara kullandığı, implantların erken dönemde
kaybedildiği, plak ve kanama indekslerinin ortalama değerlerden yüksek olduğu
ve cep derinliklerinin normal sınırlar arasında olduğu görülmüştür.

Sonuç: Sonuç olarak implant kaybında IL-1β pozitif genotipe sahip olma ve sigara içiminin önemli bir etken
olduğu izlenimi edinilmiş ancak olgu sayısının arttırılmasıyla istatistiksel
anlamlılığa ulaşılabileceği sonucuna ulaşılmıştır.

Cite this article as: Dizen F, Görmüş U,
Ergen A, Ünür M, İplik ES, Çakmakoğlu B, et al. Investigation of the IL-1 β Genotype and its Levels on Patients Treated with Osteointegrated
Dental Implants. Experimed 2019; 9(1): 11-5.

Anahtar Kelimeler

IL-1β +3953, implant, interlökin, polimorfizm

Kaynakça

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