Year 2019, Volume 44, Issue 3, Pages 463 - 471 2019-05-15

ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES
LISTERIA MONOCYTOGENES SPESİFİK FAJLARIN İZOLASYONU

PINAR ŞANLIBABA [1] , Başar UYMAZ TEZEL [2]

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It was aimed to isolate, purify and determine host ranges of lytic phages that infect Listeria monocytogenes in this study. Out of 68 samples screened, 4 positive isolates were recovered from feces, food processing waste water, fisheries waste water, and fish samples. Recovery status of the L. monocytogenes phage was found to be 5.88%. To determine host ranges of phages, soft agar overlay plaque assay was used. While eleven L. monocytogenes strains showed resistant to all four isolated phages, 24 strains were sensitive. The plaque sizes of the 4 phages against 24 L. monocytogenes strains ranged from 0.4 and 2.6 mm. None of the phages had identical host ranges. 

Bu çalışmada Listeria monocytogenes'i enfekte eden litik fajların izole edilmesi, saflaştırılması ve konakçı etkinliklerinin belirlenmesi amaçlanmıştır. Toplamda 68 örnek taranmış ve dışkı, gıda işleme atık suları, balıkçılık atık suları ve balık örneklerinden 4 pozitif izolat elde edilmiştir. Faj varlığı bakımından taranan örneklerin % 5.88’inde L. monocytogenes fajı izole edilmiştir. Faj konakçı etkinliğinin belirlenmesi amacıyla, çift tabaka agar yöntemi kullanılmıştır.  11 L. monocytogenes suşu denenen dört faja karşı dirençli bulunurken, 24 suşta ise duyarlılık saptanmıştır.  Dört fajın 24 L. monocytogenes suşuna karşı oluşturduğu faj plak çapı ise 0.4 ve 2.6 mm arasında değişmiştir. Hiçbir faj birbiri ile aynı konakçı etkinliği göstermemiştir. 

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Primary Language en
Subjects Science
Published Date 2019
Journal Section Articles
Authors

Author: PINAR ŞANLIBABA (Primary Author)
Institution: ANKARA UNIVERSITY
Country: Turkey


Author: Başar UYMAZ TEZEL
Institution: CANAKKALE ONSEKIZ MART UNIVERSITY

Supporting Institution Ankara University Scientific Research Projects Coordination Unit
Project Number 15B0443010
Dates

Publication Date: May 15, 2019

Bibtex @research article { gida522119, journal = {GIDA}, issn = {1300-3070}, eissn = {1309-6273}, address = {Gıda Teknolojisi Derneği}, year = {2019}, volume = {44}, pages = {463 - 471}, doi = {10.15237/gida.GD19036}, title = {ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES}, key = {cite}, author = {ŞANLIBABA, PINAR and UYMAZ TEZEL, Başar} }
APA ŞANLIBABA, P , UYMAZ TEZEL, B . (2019). ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES. GIDA, 44 (3), 463-471. DOI: 10.15237/gida.GD19036
MLA ŞANLIBABA, P , UYMAZ TEZEL, B . "ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES". GIDA 44 (2019): 463-471 <http://dergipark.org.tr/gida/issue/44377/522119>
Chicago ŞANLIBABA, P , UYMAZ TEZEL, B . "ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES". GIDA 44 (2019): 463-471
RIS TY - JOUR T1 - ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES AU - PINAR ŞANLIBABA , Başar UYMAZ TEZEL Y1 - 2019 PY - 2019 N1 - doi: 10.15237/gida.GD19036 DO - 10.15237/gida.GD19036 T2 - GIDA JF - Journal JO - JOR SP - 463 EP - 471 VL - 44 IS - 3 SN - 1300-3070-1309-6273 M3 - doi: 10.15237/gida.GD19036 UR - https://doi.org/10.15237/gida.GD19036 Y2 - 2019 ER -
EndNote %0 THE JOURNAL OF FOOD ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES %A PINAR ŞANLIBABA , Başar UYMAZ TEZEL %T ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES %D 2019 %J GIDA %P 1300-3070-1309-6273 %V 44 %N 3 %R doi: 10.15237/gida.GD19036 %U 10.15237/gida.GD19036
ISNAD ŞANLIBABA, PINAR , UYMAZ TEZEL, Başar . "ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES". GIDA 44 / 3 (May 2019): 463-471. https://doi.org/10.15237/gida.GD19036
AMA ŞANLIBABA P , UYMAZ TEZEL B . ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES. GIDA. 2019; 44(3): 463-471.
Vancouver ŞANLIBABA P , UYMAZ TEZEL B . ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES. GIDA. 2019; 44(3): 471-463.