Caltha palustris L. Bitkisinin Çiçek ve Yaprak Kısmının Uçucu Yağlarının Kimyasal Bileşimi ve Antimikrobiyal Aktiviteleri

Yıl 2019, Cilt: 9 Sayı: 2, 189 - 195, 15.04.2019

Tayyibe Beyza Yücel Seda Fandaklı Salih Terzioğlu Nurettin Yaylı

https://doi.org/10.17714/gumusfenbil.399711

Öz

Caltha palustris L. (Ranunculaceae) bitkisinin çiçek ve yaprak kısımlarının uçucu
yağları Clevenger aparatlı  subuharı
destilasyonu yöntemiyle elde edilmiştir. Elde edilen uçucu yağların kimyasal
bileşenleri, GC-FID ve GC-MS teknikleriyle aydınlatılmıştır. C. palustris
bitkisinin çiçek ve yaprak kısımlarının uçucu yağlarında sırasıyla; toplam 52
ve 36 bileşik bulunmuş olup; sırasıyla %95.34 ve %82.4’lük kısımları
aydınlatılmıştır. Çiçek kısmının uçucu yağının başlıca bileşenleri; oktadekanol
(%25.40), fitol (%18.56), octanol (%9.01), neofitadien (%3.65), alkol (%36.30)
ve terpenoit (%22.62) bileşiklerden oluştuğu; yaprak kısmının uçucu yağ
analizinde ise ise heptadekan (%10.05), fitol (%8.94), oktadekanol (%6.3),
heneikosan (%3.03), (E)-β-farnesan (%1.34) gibi ağırlıklı olarak hidrokarbon
(%30.89) ve terpenoit (%14.59) bileşiklerden oluştuğu tespit edilmiştir C. palustris
bitkisinin çiçek ve yaprak kısımlarından elde edilen uçucu yağların
antimikrobiyal aktiviteleri, 8 adet Gram pozitif ve Gram negatif bakteri ve
mantarlara karşı araştırılmıştır. Uçucu yağların, Candida albicans ve Saccharomyces cerevisiae’ya (8-14mm) karşı antimikotik aktivite gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Antimikrobiyal aktivite, Caltha palustris, GC-FID/MS, Uçucu yağ

Chemical Composition and Antimicrobial Activity of Essential Oil From the Flower and Leaf of Caltha palustris L.

Öz

The essential oils from flower and leaf of Caltha palustris
L. were obtained by hydrodistillation with Clevenger-type apparatus and
analyzed by GC-FID and GC-MS. Fifty-two compounds in the oil resulted from
flower of C. palustris, representing 95.34% and thirty-six compounds in the oil
obtained from leaf of C. palustris, representing 82.4%, were identified. The
main components of the volatile oil of the flower part are octadecanol
(25.40%), phytol (18.56%), octanol (9.01%) and neophytadien (3.65%) which are
alcohols (36.30%) and terpenoids (22.62%) type compounds. It was found that the
volatile oil of the leaf part consisted predominantly of hydrocarbons (30.89%)  and terpenoid (14.59%)  compounds such as heptadecane (10.05%), phytol
(8.94%), octadecanol (6.3%), heneicosane (3.03%) and  (E)-β- farnesane (1.34%) were major
constituents. The antimicrobial activities of the essential oils obtained from
flower and leaf parts of C. palustris plant were investigated against 8 Gram
positive and Gram-negative bacteria and fungi. It has been determined that
essential oils have antimycotic activity against Candida albicans and Saccharomyces cerevisiae (8-14 mm),
respectively.

Anahtar Kelimeler

Antimicrobial activity, Caltha palustris, GC-FID/MS, Essential oil

Kaynakça

• Adams, R.P., 2004, Identification of essential oil components by gas chromatography/mass Spectrometry. 4th Ed., Allured publishing Corp., Carol Stream, Illinois. 1-698.
• Ali, H., Nisar, M., Jehandar, S., 2011, Ethnobotanical study of some elite plants belonging to Dir, Kohistan Valley, Khyber Pu-khtunkhwa, Pakistan [J]. Pak J Bot, 43(2): 787-795.
• Baykal, T., Bedir, E., Calis, I., Aquino, R., Piacente S., Pizza, C., 1999, Two oleanene glycosides from the aerial parts of Caltha polypetala, Phytochemistry 51:1059-1063.
• Baytop, T. 1984. Therapy with medicinal plants in Turkey (past and present), Publications of Istanbul University, 3255(40), İstanbul, Turkey.
• Bhandari, P and Rastogi, R.P, 1984, Triterpene constituents of Caltha palustris, Phtyochemistry, 23(9); 2082-2085.
• Bhandari, P and Rastogi, R.P, 1984, Two nor-triterpene lactones from Caltha palustris, Phtyochemistry, 23(8); 1699-1702.
• Birinci, S., 2008, Doğu Karadeniz Bölgesinde Doğal Olarak Bulunan Faydalı Bitkiler ve Kullanım Alanlarının Araştırılması. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, s.143.
• Bonora, A., Tosi B., Donini A., Botta B., Bruni A., 1987, Elicitor-induced Accumulation of Protoanemonin in Caltha palustris L., Journal of Plant Physiology 131(5), 489-494.
• Cansu, T. B., Yücel, M., Sinek, K., Baltacı, C., Karaoglu, A. Ş. ve Yaylı N., 2011, Microwave Assisted Essential Oil Analysis and Antimicrobial Activity of M. Alpestris Subsp. Alpestris, Asian Journal Of Chemistry, 23(3), 1029-1031.
• Davis, P. H., 1965-1985, Flora of Turkey and the East Aegean Island Vol.:I and Supplament, Edinburg: Edinburg Univesity Press.
• Dickenmann R., 1982, Cyanogenesis in Ranunculus montanuss from the Swiss Alps [J], Bericht des Geobotanischen Institutes ETH, 49(1): 56-75.
• Ekmekçigil M., 2006, Ankara Üniversitesi Fen Fakültesi herbaryumu (ANK) Ranunculaceae familyası revizyonu, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, s.105, Ankara.
• Ellnain-Wojtaszek, M., Kowalewski, Z., Bialecka, L., 1991, Flavonoid compounds in flowers of Caltha palustris L. (Ranunculaceae), Herba Polonica 37(3-4), 125-132.
• Fancelloa, F., Zaraa, S., Petrettob, G. L., Chessab M., Addisb R., Rourkec J. P. and Pintoreb, G., 2017, Essential oils from three species of Mentha harvested in Sardinia chemical characterization and evaluation of their biological activity, International Journal of Food Properties, 20(2), 1751–1761.
• Figurkin, B.A., Khidasheli, V.D., Pidemskii, E.L., Goleneva, A.F., 1978, Triterpenoid glycosides of Caltha palustris L. and their effect on some biochemical indexes of the blood serum of rats, Rastitel'nye Resursy 14(1), 93-95.
• Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (edlr.), 2012. Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul.
• Ibanez, S., Dotterl, S., Anstett, M.C., Baudino, S., Caissard, J.C., Gallet, C. ve Despre´s, L., 2010, The role of volatile organic compounds, morphology and pigments of globeflowers in the attraction of their specific pollinating flies, New Phytologist, 188: 451–463.
• Jurgens, A. ve Dötterl S., 2004, Chemical Composition of Anther Volatiles in Ranunculaceae: Genera-Specific Profiles in Anemone, Aquılegıa, Caltha, Pulsatılla, Ranunculus and Trollius Species, American Journal of Botany, 91(12): 1969–1980.
• Kumar, P. ve Singhal V. K., 2008, Cytology of Caltha palustris L. (Ranunculaceae) from Cold Regions of Western Himalayas, Cytologia 73(2): 137–143.
• Küçükkurt, İ. ve Fidan, A.F., 2008, Saponinler ve Bazı Biyolojik Etkileri, Kocatepe Veteriner Dergisi, 1: 89-96.
• Mubashir, S., Dar M.Y., Lone B. A., Zargar M. I., Shah W. A., 2014, Anthelmintic, antimicrobial, antioxidant and cytotoxic activity of Caltha palustris var. alba Kashmir, India, Chinese Journal of Natural Medicines, 12(8): 567-572.
• NIST Chemistry Webbook, NIST Standart Reference Database Version 147.
• Radulovi´c, N.S., Dordevi´c, A.S., Zlatkovi´c, B. K., Pali´, R. M., 2009, GC-MS analyses of flower ether extracts of Prunus domestica L. and Prunus padus L. (Rosaceae), Chemical Papers, 63 (4) 377–384.
• Roner, M.R., Sprayberry, J., Spinks, M. ve Dhanji, S., 2007, Antiviral Activity Obtained from Aqueous Extracts of the Chilean oapbark tree (Quillaja saponaria Molina), J. Gen Virol, 88, 275–285.
• Schuettpelz, E. ve Hoot, S. B., 2004, Phylogeny and Biogeography of Caltha (Ranunculaceae) Based on Chloroplast and Nuclear Dna Sequences, American Journal of Botany 91(2): 247–253.
• Seago Jr, J. L., Peterson C. A., Kinsley L. J., Broderick J., 2000, Development and Structure of the Root Cortex in Caltha palustris L. and Nymphaea odorata Ait, Annals of Botany 86(3) 631-640.
• Smith Jr. C. R., Kleiman, R. , Wolff, I. A., 1968, Caltha palustris L. Seed Oil. A source of four fatty acids withcis-5-unsaturation, Lipids, 3(1): 37–42.
• Suszko, A. and Obmińska-Mrukowicz, B., 2013, Influence of polysaccharide fractions isolated from Caltha palustris L. on the cellular immune response in collagen-induced arthritis (CIA) in mice. A comparison with methotrexate, Journal of Ethnopharmacology, 145(1), 109-117.
• Tóth, A., Lakatos T., Braun M. Kiss B., 1999, Ramet distribution, leaf morphometry and elemental composition of Caltha palustris L. along a water depth gradient, Flora 194(4), 431-437.
• Tyagi, V., Patel, R., Hazarika, H., Dey P., Goswami, D. ve Chattopadhyay P., 2017, Chemical composition and bioefficacy for larvicidal and pupicidal activity of essential oils against two mosquito species, International Journal of Mosquito Research, 4(4): 112-118.
• Üçüncü, O., Baltacı, C. ve İlter Ş. M., 2016, Gladiolus italicus Miller Bitkisinin Uçucu Yağının Kimyasal Bileşimi ve Biyoaktif Özellikleri, GÜFBED/GUSTIJ, 6 (2): 150-156.
• Van der Welle, M.E.W., Niggebrugge, K., Lamers, L.P.M., Roelofs, J.G.M., 2007, Differential responses of the freshwater wetland species Juncus effusus L. and Caltha palustris L. to iron supply in sulfidic environments, Environmental Pollution, 147, 222-230.
• Vugalter M. M., Dekanosidze G. E., Dzhikiya O. D., Shashkov A. S., Kemertelidze É. P., 1988, Triterpene saponins of Caltha polypetala Glycosides G and I., Chemistry of Natural Compounds 24(2), 193–200.
• Woodell, S. R. J. ve Kootin, S.,1971, Intraspecific variation in Caltha palustris, New Phytol. 70: 173–186.
• Wink, M., 2009, Mode of action and toxicology of plant toxins and poisonous plants, Mitt. Julius Kühn-Inst. 421, 93-112.
• Yaylı, N., Yaşar, A., İskender, N. Y., Yaylı, N., Cansu T.B., Coskunçelebi, K. ve Karaoğlu, Ş., 2010, Chemical constituents and antimicrobial activities of the essential oils from Sedum pallidum var. bithynicum and S.spurium Grown in Turkey, Pharm. Biol . 48(2), 191-194
• Yeşilyurt, E. B., Çetin, Ö. ve Gürbüz, Y., 2016, Konya (Pseudodelphinium Turcicum) Mevzek Tür Eylem Planı, T.C. Orman Ve Su İşleri Bakanlığı Doğa Koruma ve Milli Parklar Genel Müdürlüğü, s.2.
• Yücel, T.B., Karaoğlu A. Ş. ve Yaylı N., 2017, Antimicrobial Activity and Composition of Rindera lanata (LAM.) Bunge var. canescens (A.D.C.) Kosn. Essential oil Obtained by Hydrodistillation and Microwave Assisted Distillation, Rec. Nat. Prod. 11:3, 328-333.
• URL-1: http://www.theplantlist.org/1.1/browse/A/Ranunculaceae/Caltha/ (Erişim tarihi: 24 Nisan 2018).
• Zelenina M.V.,1980, Localization of triterpenoid glycosides in Anemone ranunculoides L., Ficaria verna Huds. and Caltha palustris L. of the Ranunculaceae family, Rastitel'nye Resursy 16(2), 235-237.