Year 2019, Volume 4, Issue 1, Pages 67 - 73 2019-04-01

HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS

Gamze ERDOGAN [1] , Yusuf NERGİZ [2] , Elif AĞAÇAYAK [3]

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The placenta plays a role in the pathophysiology of preeclampsia. Preeclampsia is more common in multifetal pregnancies than singleton pregnancies. In this study, we aimed to investigate the histopathology of normotensive and preeclamptic placentas and the localization of alkaline phosphatase activity. In our study, 10 normotensive and 10 preeclamptic, totally 20 placentas were obtained.Paraffin sections were stained with Hematoxylin-Eosin, Masson trichrome and PAS for histopathological examination.Remaining sections were then stained via Gomori’s method and micrographed under light microscope. Sections of the control group observed normal histologically structure.The  alkaline phosphatase reaction was evident in the inner and outer membranes of the syncytiotrophoblasts. There was a significant increase in the number of syncytial knots, terminal villi and syncytial bridges in the preeclamptic placenta sections. Marked thickening of the trophoblast basal membranes were observed. Alkaline phosphatase reaction in preeclampsia group: The localization of alkaline phosphatase in the inner and outer membranes of the syncytiotrophoblasts was reduced. In placentas of preeclampsia group, a significant increase in syncytial knot, syncytial bridge were observed. The level of alkaline phosphatase enzyme in preeclamptic placentas was found to be lower compared to normotensive placenta.

Placenta, Preeclampsia, Alkaline phosphatase, Light microscope
  • [1] Stead SM, Stead LG, Kaufman MS, Feig RL, and Johnson NC. Complications of pregnancy, In: First Aid for the Obstetrics and Gynecology, McGraw-Hill Book Company, NewYork, London, 2002.
  • [2] Granger JP, Alexander BT, Linas MT, Bennet WA and Khalil RA Pathophysiology of preeclampsia linking placental ischemia/hypoxia with microvascular dysfunction. Microcirc., 9 (3), 147-160, 2002.
  • [3] Rodin A, Duncan A, Quartero HWP, Pistofidis G, Mashiter G, Whitetaken K, Crook D, Stevenson JC, Chapman MG and Fogelman I. Serum Concentration of Alkaline Phosphatase Isoenzymes and Osteocalcin in Normal Preganacy. Journal of Clinical Endocrinological Methods, 68, 1123-1127, 1989.
  • [4] J. D. Boyd and W. J. Hamilton, The human placenta, Heffer, Cambridge, 1970.
  • [5] Ursula N. lister: The localization of placental enzymes whit electron microscope. J. obstet. Gynec. Britt. Cwlht., 74, 34-29, 1967.
  • [6] Fox N. F. Kharkongar: Enzyme histochermistry of the Hofbauer cells of the human placenta. J. obs. Gynec. Britt. Cwlht., 76, 918-921, 1969.
  • [7] Ralph, M. Wynn: Derivation and ultrastructure of so-called Hofbauer cell. Am. J. obs. And gyn. , 97, 235-248, 1967.
  • [8] G. Wielangta. R.G.J. Willighagen: Histochemistry of the syncytotrofoblast and the stroma in the normal full-term placenta. Am. J. Obs. Gynec., 84, 1059, 1962.
  • [9] M. Wachstein, J.G. Meager and J. Ortis: Enzymatic histochemistry of the term human placenta. Am. J.Obs. Gynec., 87, 13, 1963.
  • [10] De Duve: Lysosomes, a new group of cytoplasmic particles. Edited by Hayashi T, Ronald pres, New York, 1999.
  • [11] Messer RH, Heat stable alkaline phosphatase as an index of placental function. American J. Obstet. & Gynecol., 15, 459-465, 1997.
  • [12] Luis A.The normal and abnormal placentae, American J. Obstet. & Gynecol., 118, 273-275, 1974.
  • [13] Jeacock M K, Morris N F. The activity of alkaline and acid phosphatase in the human placenta. Journal of Obstetrics and Gynaecology, 70, 267-273, 1993.
  • [14] Curzen P. Variation in enzyme histochemistry of Placenta. Journal of Obstetrics and Gynaecology, 71, 388-399, 1994.
  • [15] Dempsey E W, Wislocki G B. Further observations on distribution of phosphatases in mammalian placentas. The American Journal of Anatomy. 80(1), 1-3, 1987.
  • [16] Mangal A, Shrivastava P, Gaur U, Jain A, Goyal U, Rath G. Histochemical analysis of placental alkaline phosphatase in Hypertensive Disorders complicating Pregnancy. J. Anat. Soc. India, 54(2), 1-9, 2005.
  • [17] Saleh RA, Dkhıl MAM. Structural changes of placenta in preeclamptic patients: Light and electron microscopic study. Turk J Med Sci., 38(3), 219-225, 2008.
  • [18] Majumdar S, Dasgupta H, Bhattacharya K, Bhattacharya A. A study of placenta in normal and hypertensive pregnancies. J Anat Soc India, 54(2), 1-9, 2005.
  • [19] Elpek GÖ, Karaveli Ş, Keleş N. Preeklampsili olguların term plasentalarında villöz trofoblast proliferasyonunun incelenmesi. Türk Patoloji Dergisi, 16(1-2), 10-12, 2000.
  • [20] Yuping Wang Y, Alexander JS. Placental pathophysiology in preeclampsia. Pathophysiology, 6, 261-270, 2000.
Primary Language en
Subjects Health Care Sciences and Services
Journal Section Article
Authors

Orcid: 0000-0002-5854-8517
Author: Gamze ERDOGAN
Institution: Dicle University
Country: Turkey


Orcid: 0000-0002-9988-9385
Author: Yusuf NERGİZ (Primary Author)
Institution: Dicle University
Country: Turkey


Orcid: 0000-0002-5854-8517
Author: Elif AĞAÇAYAK
Institution: Dicle University

Dates

Application Date: February 28, 2019
Acceptance Date: March 25, 2019
Publication Date: April 1, 2019

Bibtex @research article { ijhsrp533771, journal = {International Journal of Health Services Research and Policy}, issn = {}, eissn = {2602-3482}, address = {Rojan GÜMÜŞ}, year = {2019}, volume = {4}, pages = {67 - 73}, doi = {10.23884/ijhsrp.2019.4.1.07}, title = {HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS}, key = {cite}, author = {ERDOGAN, Gamze and NERGİZ, Yusuf and AĞAÇAYAK, Elif} }
APA ERDOGAN, G , NERGİZ, Y , AĞAÇAYAK, E . (2019). HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS. International Journal of Health Services Research and Policy, 4 (1), 67-73. DOI: 10.23884/ijhsrp.2019.4.1.07
MLA ERDOGAN, G , NERGİZ, Y , AĞAÇAYAK, E . "HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS". International Journal of Health Services Research and Policy 4 (2019): 67-73 <http://dergipark.org.tr/ijhsrp/issue/44323/533771>
Chicago ERDOGAN, G , NERGİZ, Y , AĞAÇAYAK, E . "HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS". International Journal of Health Services Research and Policy 4 (2019): 67-73
RIS TY - JOUR T1 - HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS AU - Gamze ERDOGAN , Yusuf NERGİZ , Elif AĞAÇAYAK Y1 - 2019 PY - 2019 N1 - doi: 10.23884/ijhsrp.2019.4.1.07 DO - 10.23884/ijhsrp.2019.4.1.07 T2 - International Journal of Health Services Research and Policy JF - Journal JO - JOR SP - 67 EP - 73 VL - 4 IS - 1 SN - -2602-3482 M3 - doi: 10.23884/ijhsrp.2019.4.1.07 UR - https://doi.org/10.23884/ijhsrp.2019.4.1.07 Y2 - 2019 ER -
EndNote %0 International Journal of Health Services Research and Policy HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS %A Gamze ERDOGAN , Yusuf NERGİZ , Elif AĞAÇAYAK %T HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS %D 2019 %J International Journal of Health Services Research and Policy %P -2602-3482 %V 4 %N 1 %R doi: 10.23884/ijhsrp.2019.4.1.07 %U 10.23884/ijhsrp.2019.4.1.07
ISNAD ERDOGAN, Gamze , NERGİZ, Yusuf , AĞAÇAYAK, Elif . "HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS". International Journal of Health Services Research and Policy 4 / 1 (April 2019): 67-73. https://doi.org/10.23884/ijhsrp.2019.4.1.07
AMA ERDOGAN G , NERGİZ Y , AĞAÇAYAK E . HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS. IJHSRP. 2019; 4(1): 67-73.
Vancouver ERDOGAN G , NERGİZ Y , AĞAÇAYAK E . HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS. International Journal of Health Services Research and Policy. 2019; 4(1): 73-67.