Multi-Mode Assessment Approach on Anti-Cancer Potency of Vanadium on Breast Cancer Cells

Yıl 2018, Cilt: 77 Sayı: 2, 47 - 54, 02.12.2018

Canan Vejselova Sezer

Öz

DOI: 10.26650/EurJBiol.2018.18007

Objective: The anti-cancer activities of
vanadium and its compounds have been widely investigated in cancer research
recently. This is mainly attributed to vanadium and its compounds’ near ideal
properties for being an anti-cancer agent. Most of the current classical
chemotherapeutics used in cancer therapy are known to bring numerous strong
side effects. Thus, there is a need to discover new drugs which have mild or no
side effects and which are effective in low doses. For the treatment of breast
cancer (a disease with a difficult and costly treatment process, high
mortality, and which is especially prevalent in woman), novel drugs and
approaches are required. With this in mind, this study investigates the
potential therapeutic efficacies of vanadyl sulphate, a member of the vanadium
compounds with ideal anti-cancer properties such as cytotoxicity,
antiproliferative and proapoptotic activities on human breast adenocarcinoma
cells (MCF-7) including morphological and ultrastructural changes.

Materials and Methods: A MTT colorimetric
assay was used for cell viability assessment. Morphological and ultrastructural
changes were evaluated using confocal and transmission electron microscopy
methods, respectively. The apoptosis stimulating property of vanadyl sulphate
was tested under a flow cytometry. And also, cell cycle and proliferation
inhibitory effects were examined using the immunohistochemistry technique. 

Anahtar Kelimeler

Breast cancer, vanadium, cytotoxic activity

Kaynakça

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