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Ekzoftalmuslu Gökkuşağı Alabalıklar (Oncorhynchus mykiss)’ın Gözlerinden Lacctococcus garvieae’nın İzolasyonu

Yıl 2019, Cilt: 4 Sayı: 1, 27 - 33, 30.04.2019
https://doi.org/10.35229/jaes.527258

Öz

Kaynakça

  • Altun, S., Diler, O. and Adiloglu, A.K. (2004). Genotyping of Lactococcus garvieae strainsfrom rainbow trout (Oncorhynchus mykiss) by16S rDNA sequencing. Bulletin of the European Association of Fish Pathologists, 24, 119-125.
  • Austin, B. and Austin, D.A. (1999). BacterialFish Pathogens: Disease in Farmed and Wild Fish, 3rd ed. Springer-Praxis, Chichester, UK.
  • Austin, B. and Austin, D.A. (2007). BacterialFish Pathogens: Disease in Farmed and Wild Fish, 4th ed. Springer-Praxis, Chichester,UK.
  • Avcı, H., Aydoğan, A., Tanrıkul, T.T. & Birincioğlu, S.S. (2010). Pathological and Microbiological Investigations in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) Naturally Infected with Lactococcus garvieae. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16(Suppl-B), 313-318.
  • Balta, F. (2016). Phenotypic, serotypic and genetic characterization and antimicrobial susceptibility determination of Vibrio anguillarum, isolated from cultured sea bass (Dicentrarchus labrax L., 1758) in the southeast Black Sea, Turkey. Fresenius Environmental Bulletin, 25(10), 4393-4400.
  • Cagırgan, H. and Tanrıkul, T.T. (1995). A new problem Enterococcus-like Infection in Rainbow Trout (Oncorhynchus mykiss) Farms in Turkey (in Turkish). Veteriner Kontrol ve Arastirma Enstitusu Dergisi, 33, 9-19.
  • Cagırgan, H. (2004). Biotyping of Lactococcus garvieae Isolated from Turkey. Ege Journal of Fisheries and Aquatic Sciences, 21(3-4), 267-269.
  • CLSI (2008). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria İsolated From Animals; Approved Standa-Third Edition. CLSI document M31-A3. Vol. 38, No.8, Wayne, PA.
  • CLSI (20013). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI document M100-S23. Vol. 33, No.1, Wayne, PA.
  • Collins, M.D., Ash, C., Farrow, J.A.E., Wallbanks, S. and Williams, A.M, (1989). 16S ribosomal ribonucleic acid sequences analysis of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov. Journal of Applied Microbiology, 73, 433-437.
  • Didinen, B.I., Yardımcı, B., Onuk, E.E., Metin S. & Yıldırım, P. (2014). Naturally Lactococcus garvieae infection in rainbow trout (Oncorhyncus mykiss Walbaum, 1792): new histopathological observations, phenotypic and molecular identifcation. Revue de Médecine Vétérinaire, 165(1-2), 12-19.
  • Diler, O., Altun, S., Adiloglu, A.K., Kubilay, A. and Isikli, B.I. (2002). First occurrence of streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bulletin of the European Association of Fish Pathologists, 22, 21-26.
  • Eldar, A. & Ghittino, C. (1999). Lactococcus garvieae and Streptococcus iniae infection in rainbow trout (Oncorhynchus mykiss): similar but different diseases. Diseases of Aquatic Organisms, 36, 227-231.
  • Kayis S., Capkin E. , Balta F., Altinok I. (2009). Bacteria in rainbow trout (Oncorhynchus mykiss) in the southern Black Sea Region of Turkey-A survey. The Israeli Journal of Aquaculture-Bamidgeh, 61, 339-344.
  • Kubilay, A., Altun, S., Ulukoy, G., Diler, O. (2005). Determination of antimicrobial susceptibility of Lactococcus garvieae strains. S.D.Ü. Eğirdir Su Ürünleri Fakültesi Dergisi, 1, 39-48.
  • Kusuda, R. and Salati, F. (1999). Enterococcus seriolicida and Streptococcus iniae. pp. 303-317. In: P.T.K. Woo, D.W. Bruno (eds.). Fish Diseases and Disorders Viral, Bacterial and Fungal Infections, vol. 3. CABI Publ.
  • Öztürk, T., Didinen, B.I., Doğan, G., Özer, A. & Bircan, R. (2013). Lactococcosis in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) in the middle Black Sea Region in Turkey and antimicrobial susceptibility of the etiological agent, Lactococcus garvieae. Journal of Etlik Veterinary Microbiology, 24, 7-12.
  • Tsai, M.A., Wang, P.C., Yoshida, T., Liaw, L.L. and Chen, S.C. (2013). Development of a sensitive and specific LAMP PCR assay for detection of fish pathogen Lactococcus garvieae. Diseases of Aquatic Organisms, 102, 225-235.
  • Zlotkin, A., Eldar, A., Ghittino, C. and Bercovier, H. (1998). Identification of Lactococcus garvieae by PCR. Journal of Clinical Microbiology, 36, 983-985.
  • Vela, A.I., Va´zquez, J., Gibello, A., Blanco, M.M., Moreno, M.A., Lie´bana, P., Albendea, C., Alcala, B., Mendez, A., Domi´nguez, L. & Ferna´ndez-Garayza´bal, J. F. (2000). Phenotypic and genetic characterization of Lactococcus garvieae isolated in Spain from Lactococcosis outbreaks and comparison with isolates of other countries and sources. Journal of Clinical Microbiology, 38(10), 3791-3795.
  • Vendrell, D., Balca´zar, J.L., Ruiz-Zarzuela, I. de Blas, I. , Girone´s, O. & Mu´zquiz, J.L. (2006). Lactococcus garvieae in fish: A review. Comparative Immunology, Microbiology & Infectious Diseases, 29, 177-198.

The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia

Yıl 2019, Cilt: 4 Sayı: 1, 27 - 33, 30.04.2019
https://doi.org/10.35229/jaes.527258

Öz

In this study, fish were
sampled from 9 different fish farms in the dam lakes in the Eastern Black Sea
Region between January 2016 and September 2018. It was isolated twenty-two bacteria
isolates from eyes of diseased rainbow trout (Oncorhynchus mykiss). All
isolates identification was carried out by using conventional biochemical
tests, api 20 strep tests and PCR tests. ATCC 43921 reference strain was used
as a positive control. According to results of the api 20 strep test, it was
determined that 20 isolates from 22 bacterial isolates were Lactococcus garvieae. The PZR test was
confirmed by using the pLG-1 and pLG-2 reference genes (16S rRNA) specific for
the L. garvieae species. As a result
of the study, it was confirmed that there were 20 L. garvieae from 22 strains isolated from rainbow trout in the dam
lakes fish farms. The antibiotic susceptibility test results showed that L. garvieae isolates were resistant %100
to streptomycin, sulfamethoxazole and sulfamethoxazole+trimethoprim, 94.2% to
ampicillin, 72.5% to oxytetracycline, 55.07% to erythromycin and 43.5% to
oxolinic acid, 26.09% to enrofloxacin, % 20.3 doxycycline, 8,7% to amoxicillin
and florphenicol. The most effective antibiotic was found to be florfenicol,
amoxicillin, doxisicline and enrofloxacin.

Kaynakça

  • Altun, S., Diler, O. and Adiloglu, A.K. (2004). Genotyping of Lactococcus garvieae strainsfrom rainbow trout (Oncorhynchus mykiss) by16S rDNA sequencing. Bulletin of the European Association of Fish Pathologists, 24, 119-125.
  • Austin, B. and Austin, D.A. (1999). BacterialFish Pathogens: Disease in Farmed and Wild Fish, 3rd ed. Springer-Praxis, Chichester, UK.
  • Austin, B. and Austin, D.A. (2007). BacterialFish Pathogens: Disease in Farmed and Wild Fish, 4th ed. Springer-Praxis, Chichester,UK.
  • Avcı, H., Aydoğan, A., Tanrıkul, T.T. & Birincioğlu, S.S. (2010). Pathological and Microbiological Investigations in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) Naturally Infected with Lactococcus garvieae. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16(Suppl-B), 313-318.
  • Balta, F. (2016). Phenotypic, serotypic and genetic characterization and antimicrobial susceptibility determination of Vibrio anguillarum, isolated from cultured sea bass (Dicentrarchus labrax L., 1758) in the southeast Black Sea, Turkey. Fresenius Environmental Bulletin, 25(10), 4393-4400.
  • Cagırgan, H. and Tanrıkul, T.T. (1995). A new problem Enterococcus-like Infection in Rainbow Trout (Oncorhynchus mykiss) Farms in Turkey (in Turkish). Veteriner Kontrol ve Arastirma Enstitusu Dergisi, 33, 9-19.
  • Cagırgan, H. (2004). Biotyping of Lactococcus garvieae Isolated from Turkey. Ege Journal of Fisheries and Aquatic Sciences, 21(3-4), 267-269.
  • CLSI (2008). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria İsolated From Animals; Approved Standa-Third Edition. CLSI document M31-A3. Vol. 38, No.8, Wayne, PA.
  • CLSI (20013). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI document M100-S23. Vol. 33, No.1, Wayne, PA.
  • Collins, M.D., Ash, C., Farrow, J.A.E., Wallbanks, S. and Williams, A.M, (1989). 16S ribosomal ribonucleic acid sequences analysis of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov. Journal of Applied Microbiology, 73, 433-437.
  • Didinen, B.I., Yardımcı, B., Onuk, E.E., Metin S. & Yıldırım, P. (2014). Naturally Lactococcus garvieae infection in rainbow trout (Oncorhyncus mykiss Walbaum, 1792): new histopathological observations, phenotypic and molecular identifcation. Revue de Médecine Vétérinaire, 165(1-2), 12-19.
  • Diler, O., Altun, S., Adiloglu, A.K., Kubilay, A. and Isikli, B.I. (2002). First occurrence of streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bulletin of the European Association of Fish Pathologists, 22, 21-26.
  • Eldar, A. & Ghittino, C. (1999). Lactococcus garvieae and Streptococcus iniae infection in rainbow trout (Oncorhynchus mykiss): similar but different diseases. Diseases of Aquatic Organisms, 36, 227-231.
  • Kayis S., Capkin E. , Balta F., Altinok I. (2009). Bacteria in rainbow trout (Oncorhynchus mykiss) in the southern Black Sea Region of Turkey-A survey. The Israeli Journal of Aquaculture-Bamidgeh, 61, 339-344.
  • Kubilay, A., Altun, S., Ulukoy, G., Diler, O. (2005). Determination of antimicrobial susceptibility of Lactococcus garvieae strains. S.D.Ü. Eğirdir Su Ürünleri Fakültesi Dergisi, 1, 39-48.
  • Kusuda, R. and Salati, F. (1999). Enterococcus seriolicida and Streptococcus iniae. pp. 303-317. In: P.T.K. Woo, D.W. Bruno (eds.). Fish Diseases and Disorders Viral, Bacterial and Fungal Infections, vol. 3. CABI Publ.
  • Öztürk, T., Didinen, B.I., Doğan, G., Özer, A. & Bircan, R. (2013). Lactococcosis in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) in the middle Black Sea Region in Turkey and antimicrobial susceptibility of the etiological agent, Lactococcus garvieae. Journal of Etlik Veterinary Microbiology, 24, 7-12.
  • Tsai, M.A., Wang, P.C., Yoshida, T., Liaw, L.L. and Chen, S.C. (2013). Development of a sensitive and specific LAMP PCR assay for detection of fish pathogen Lactococcus garvieae. Diseases of Aquatic Organisms, 102, 225-235.
  • Zlotkin, A., Eldar, A., Ghittino, C. and Bercovier, H. (1998). Identification of Lactococcus garvieae by PCR. Journal of Clinical Microbiology, 36, 983-985.
  • Vela, A.I., Va´zquez, J., Gibello, A., Blanco, M.M., Moreno, M.A., Lie´bana, P., Albendea, C., Alcala, B., Mendez, A., Domi´nguez, L. & Ferna´ndez-Garayza´bal, J. F. (2000). Phenotypic and genetic characterization of Lactococcus garvieae isolated in Spain from Lactococcosis outbreaks and comparison with isolates of other countries and sources. Journal of Clinical Microbiology, 38(10), 3791-3795.
  • Vendrell, D., Balca´zar, J.L., Ruiz-Zarzuela, I. de Blas, I. , Girone´s, O. & Mu´zquiz, J.L. (2006). Lactococcus garvieae in fish: A review. Comparative Immunology, Microbiology & Infectious Diseases, 29, 177-198.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Fikri Balta 0000-0002-1823-5823

Zeynep Dengiz Balta Bu kişi benim 0000-0002-7795-828X

Yayımlanma Tarihi 30 Nisan 2019
Gönderilme Tarihi 14 Şubat 2019
Kabul Tarihi 9 Nisan 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 4 Sayı: 1

Kaynak Göster

APA Balta, F., & Dengiz Balta, Z. (2019). The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia. Journal of Anatolian Environmental and Animal Sciences, 4(1), 27-33. https://doi.org/10.35229/jaes.527258

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