Year 2018, Volume 1, Issue 3, Pages 60 - 60 2018-12-08

Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy

Wallance M. PAZIN [1] , Vita SOLOVYEVA [2] , Tibebe LEMMA [3] , Bjarke JØRGENSEN [4] , Carlos José L. CONSTANTINO [5] , Jonathan BREWER [6]

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Artepillin C, the major component of the Brazilian green propolis, has become the subject of great biological interest among all the secondary metabolites found in that propolis, especially due to its anticancer property. Biophysical studies have shown that the presence of two prenylated groups in the molecular structure of Artepillin C enhances its affinity for lipophilic environment1, which is in agreement with biological assays reported in the literature, suggesting that Artepillin C is absorbed through cell membranes2. The purpose of this study was to obtain for the first time a direct visualization of Artepillin C internalization into fibroblast cells by means of Coherence Anti-Stokes Raman Scattering (CARS), a powerful microscopy technique with a combination of two pulsed laser beams which induces a vibrational motion of a specific compound, generating an intrinsic image3. A detailed vibrational characterization of Artepillin C has been carried out using Raman microspectroscopy and surface-enhanced Raman scattering (SERS) in combination with quantum chemical calculation based on LANL2DZ/B3LYP level of theory.  The success use of CARS microscopy was confirmed by collecting Artepillin C images from dry and hydrated films of the compound, while the intrinsic images of Artepillin C internalized into the cells was compared to confocal fluorescence microscopy images by using a cell-membrane staining compound. With effect, this study shows for the first time a direct visualization of the major compound of green propolis into the cells by means of CARS microscopy.

 

Acknowledgements: FAPESP (2017/23426-4) and DaMBIC (University of Southern Denmark).
Fibroblast Cells, CARS Microscopy, Brazilian green propolis
  • 1. Pazin, W. M. et al. (2017) Interaction of Artepillin C with model membranes. Eur. Biophys. J. 46: 383–393.
  • 2. Paulino, N. et al. 2008 Anti-inflammatory effects of a bioavailable compound, Artepillin C, in Brazilian propolis. Eur. J. Pharmacol. 587: 296–301.
  • 3. Evans, C. L. & Xie, X. S. (2008) Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine. Annu. Rev. Anal. Chem. 1: 883–909.
Primary Language en
Subjects
Journal Section 2nd Propolis Conference Special Issue
Authors

Author: Wallance M. PAZIN

Author: Vita SOLOVYEVA

Author: Tibebe LEMMA

Author: Bjarke JØRGENSEN

Author: Carlos José L. CONSTANTINO

Author: Jonathan BREWER

Bibtex @conference paper { jan489794, journal = {Journal of Apitherapy and Nature}, issn = {}, eissn = {2667-4734}, address = {Oktay YILDIZ}, year = {2018}, volume = {1}, pages = {60 - 60}, doi = {}, title = {Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy}, key = {cite}, author = {PAZIN, Wallance M. and SOLOVYEVA, Vita and LEMMA, Tibebe and JØRGENSEN, Bjarke and CONSTANTINO, Carlos José L. and BREWER, Jonathan} }
APA PAZIN, W , SOLOVYEVA, V , LEMMA, T , JØRGENSEN, B , CONSTANTINO, C , BREWER, J . (2018). Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy. Journal of Apitherapy and Nature, 1 (3), 60-60. Retrieved from http://dergipark.org.tr/jan/issue/40577/489794
MLA PAZIN, W , SOLOVYEVA, V , LEMMA, T , JØRGENSEN, B , CONSTANTINO, C , BREWER, J . "Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy". Journal of Apitherapy and Nature 1 (2018): 60-60 <http://dergipark.org.tr/jan/issue/40577/489794>
Chicago PAZIN, W , SOLOVYEVA, V , LEMMA, T , JØRGENSEN, B , CONSTANTINO, C , BREWER, J . "Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy". Journal of Apitherapy and Nature 1 (2018): 60-60
RIS TY - JOUR T1 - Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy AU - Wallance M. PAZIN , Vita SOLOVYEVA , Tibebe LEMMA , Bjarke JØRGENSEN , Carlos José L. CONSTANTINO , Jonathan BREWER Y1 - 2018 PY - 2018 N1 - DO - T2 - Journal of Apitherapy and Nature JF - Journal JO - JOR SP - 60 EP - 60 VL - 1 IS - 3 SN - -2667-4734 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Apitherapy and Nature Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy %A Wallance M. PAZIN , Vita SOLOVYEVA , Tibebe LEMMA , Bjarke JØRGENSEN , Carlos José L. CONSTANTINO , Jonathan BREWER %T Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy %D 2018 %J Journal of Apitherapy and Nature %P -2667-4734 %V 1 %N 3 %R %U
ISNAD PAZIN, Wallance M. , SOLOVYEVA, Vita , LEMMA, Tibebe , JØRGENSEN, Bjarke , CONSTANTINO, Carlos José L. , BREWER, Jonathan . "Direct Visualization of Artepillin C into Fibroblast Cells via CARS Microscopy". Journal of Apitherapy and Nature 1 / 3 (December 2018): 60-60.