Aggregates of α-synuclein in brain tissue homogenates measured by newly designed Multimer-PAGE techniques

Yıl 2019, Cilt 11 Supp 1 (BRS), 8 - 8, 21.06.2019

Jumana Saleh

https://doi.org/10.37212/jcnos.584670

Öz

Atypical aggregation of α-synuclein in the brain is linked to Parkinson’s disease (PD) progression. Mutimers of α-synuclein may be important for the biological function of α-synuclein. Currently, reliable methods to compare α-synuclein multimer abundance in biological samples are limited and sophisticated. A new technique termed “multimer-PAGE,” was designed by Killinger and Moszczynska, which combines in-gel chemical cross-linking with several common electrophoretic techniques to measure the multimerization of α-synuclein in complex biological samples of brain tissue lysates. Using this technique, it was possible to compare the ratio of α-synuclein multimers between brain tissue samples without the need for specialized equipment. 

Anahtar Kelimeler

Brain, Multimer-PAGE techniques, Parkinson’s disease, α-synuclein

Kaynakça

• Sulaiman MA, Al-Farsi YM, Al-Khaduri MM, Waly MI, Saleh J, Al-Adawi S. Psychological burden among women with polycystic ovarian syndrome in Oman: a case-control study. Int J Womens Health. 2017;9:897-904.