Synthesis of Cystein-Gold Nanoparticles as Nanocarriers of Disulfıram used in Alcohol Treament

Yıl 2019, Cilt: 9 Sayı: 1, 479 - 486, 01.03.2019

Fatma Bayrakçeken Nişancı

https://doi.org/10.21597/jist.467229

Öz

Disulfiram (DS) is a carbamate derivative used
as an alcohol deterrent. It is a relatively non-toxic substance when
administered alone, but it significantly changes metabolism with alcohol.
Disulfiram acts by inhibiting aldehyde dehydrogenase. If alcohol is used after
disulfiram is administered, the concentration of acetaldehyde increases,
followed by systemic vasodilatation, respiratory distress, nausea, hypotension,
and other symptoms (acetaldehyde syndrome). It is possible that disulfiram is
acting more rapidly with nanostructures such as gold nanoparticles (Au NP).
Gold nanoparticles are higher potency non-toxic biomarkers when compared to
quantum dots, and in this study, cysteine ​​(Cys) is focused on synthesis with
Au NP as a reducing and protective agent. Gold nanoparticles of about 5 nm in
diameter were conjugated to cysteine ​​(Cys) and conjugated to Cys-Au NPs as
nanostructures of disulfiram, (Synthesis of gold nanoparticles, mixing in fresh
Cys solutions and mixing the mixture overnight at 37 ° C in a water bath) were characterized
by scanning electron microscopy (SEM), atomic force microscopy (AFM), permeable
electron microscopy (TEM), FT-IR, raman and UV-Vis
spectroscopy.

Anahtar Kelimeler

Gold nanoparticles, Disulfiram, S-Au interaction, Cysteine

Alkol Tedavisinde Kullanılan Disülfiram’ın Nanotaşıyıcısı Olarak Sistein-Altın Nanopartiküllerin Sentezi

Öz

Disülfiram (DS), alkol caydırıcı
olarak kullanılan bir karbamat türevidir. Tek başına uygulandığında nispeten
toksik olmayan bir maddedir, ancak alkol ile birlikte metabolizmayı belirgin
bir şekilde değiştirmektedir. Disülfiram, aldehit
dehidrojenazını inhibe ederek etki etmektedir. Disülfiram uygulandıktan sonra
alkol alınırsa, kanda asetaldehit konsantrasyonu artar, ardından sistemik
vazodilatasyon, solunum güçlüğü, bulantı, hipotansiyon ve diğer semptomlar
(asetaldehit sendromu) izlenir. Disülfiramın daha hızlı bir şekilde etki
göstermesi altın nanopartiküller (Au NP) gibi nanotaşıyıcılarla mümkün
olmaktadır. Altın nanopartiküller, kuantum noktalarla karşılaştırıldığında daha
yüksek potansiyelli toksik olmayan biyomarkerlardır ve bu çalışma kapsamında,
sisteinin (Cys) indirgeyici ve koruyucu ajan olarak Au NP ile sentezine
odaklanılmıştır. Yaklaşık 5 nm çapında altın
nanopartiküller, Cys ile modifiye edilen ve disülfiramın nanotaşıyıcısı olarak
Cys-Au NP’ler şeklinde konjugasyonu gerçekleştirilerek, (Altın nanopartiküllerin
sentezi, taze Cys solüsyonlarına karıştırılarak karışım gece boyunca 37°C'de
bir su banyosu içinde karıştırılarak) özellikleri taramalı elektron mikroskobu
(SEM), atomik kuvvet mıkroskobu (AFM) , geçirgen elektron mıkroskobu (TEM),
FT-IR, raman ve UV-Vis spektroskopisi ile değerlendirildi.

Anahtar Kelimeler

Altın nanopartikül, Disülfiram, S-Au etkileşimi, Sistein

Kaynakça

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