Year 2016, Volume 3, Issue 3, Pages 329 - 348 2017-01-08

FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION

Nimet Bölgen [1] , Zeynep Çetinkaya [2] , Didem Demir [3]

416 773

Tissue engineering aims regenerating damaged tissues by using porous scaffolds, cells and bioactive agents. The scaffolds are produced from a variety of natural and synthetic polymers. Collagen is a natural polymer widely used for scaffold production in the late years because of its being the most important component of the connective tissue and biocompatibility. Cryogelation is a relatively simple technique compared to other scaffold production methods, which enables to produce interconnected porous matrices from the frozen reaction mixtures of polymers or monomeric precursors. Considering these, collagen was isolated in this study from fish skin which is a non-commercial waste material, and scaffolds were produced from this collagen by cryogelation method. By SEM analysis, porous structure of collagen, and by UV-Vis analysis protein structure was proven, and by Zeta potential iso-electrical point of the protein was determined, and,  Amit A, Amit B, Amit I, Amit II and Amit III characteristical peaks were demonstrated by FTIR analysis. The collagen isolation yield was, 14.53% for acid soluble collagen and 2.42% for pepcin soluble collagen. Scaffolds were produced by crosslinking isolated acid soluble collagen with glutaraldehyde at cryogenic conditions. With FTIR analysis, C=N bond belonging to gluteraldehyde reaction with collagen was found to be at 1655 cm-1. It was demonstrated by SEM analysis that collagen and glutaraldeyhde concentration had significant effects on the pore morphology, diameter and wall thickness of the cryogels, which in turned changed the swelling ratio and degradation profiles of the matrices. In this study, synthesis and characterization results of a fish skin isolated collagen cryogel scaffold that may be potentially used in the regeneration of damaged tissues are presented.  
  • Sidi AB, Thomas MB, Philippe R. Advances in the design of macroporous polymer scaffolds for potential applications in dentistry. 2013 Dec;43(6):251-61. DOI: 10.5051/jpis.2013.43.6.251
  • Bhat S, Tripathi A, Kumar A. Supermacroprous chitosan–agarose–gelatin cryogels: in vitro characterization and in vivo assessment for cartilage tissue engineering. Journal of the Royal Society Interface. 2011 Apr;8:540-54. DOI: 10.1098/rsif.2010.0455
  • Martíneza A, Blancoa MD, Davidenkob N, Cameronb RE. Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties. Carbohydrate Polymers. 2015 May;132:606-19. DOI: 10.1016/j.carbpol.2015.06.084
  • Sadowska M, Koodziejska I, Niecikowska C. Isolation of collagen from the skins of Baltic cod (Gadus morhua). Food Chemistry. 2003 Aug; 81:257-62. DOI: 10.1016/S0308-8146(02)00420-X
  • Elango J, Bin B, Yongshi B, Yu Z, Qingbo Z, Wenhui W. Type II Collagen and Gelatin from Silvertip Shark (Carcharhinus albimarginatus) Cartilage: Isolation, Purification, Physicochemical and Antioxidant Properties. Marine Drugs. 2014 June;12:3852-73. DOI:10.3390/md12073852.
  • Wang L, An X, Xın Z, Zhao L, Hu Q, Isolation and Characterization of Collagen from the Skin of Deep-Sea Redfish (Sebastes mentella). Journal of Food Science. 2007 Oct;72(8):450-55. DOI: 10.1111/j.1750-3841.2007.00478.x.
  • Singh P, Benjakul S, Maqsood S, Kishimura H. Isolation and characterisation of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chemistry. 2011 Jan;124:97-105. DOI: 10.1016/j.foodchem.2010.05.111.
  • Shahid M. Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinus mrigala. Journal of Food Science and Technology. 2015 July;52(7):4296-305. DOI 10.1007/s13197-014-1520-6.
  • Lozinsky V, Galaev I. Y, Plieva FM, Savina IN, Jungvid H, Mattiasson B. Polymeric cryogels as promising materials of biotechnological interest. TRENDS in Biotechnology. 2003 Oct;21(10):445-51. DOI:10.1016/j.tibtech.2003.08.002.
  • Benjakul S, Thiansilakul Y, Visessanguan W, Roytrakul S, Kishimura H, Prodprand T, Meesaned J. Extraction and characterisation of pepsin-solubilised collagens from the skin of bigeye snapper (Priacanthus tayenus and Priacanthusmacracanthus). Journal of the Science of Food and Agriculture. 2010 Sept;90:132-38. DOI 10.1002/jsfa.3795.
  • Kemençe N, Bölgen N. Journal of Tissue Engineering and Regenerative Medicine. 2013. DOI: 10.1002/term.1813.
  • Wang B, Wang Y-W, Chi C-F, Luo H-Y, Deng S-G, Ma J-Y. Isolation and Characterization of Collagen and Antioxidant Collagen Peptides from Scales of Croceine Croaker (Pseudosciaena crocea). Marine Drugs. 2013 Nov;11:4641-661. DOI: 10.3390/md11114641
  • Wang L, An X, Yang F, Xin Z, Zhao L, Hu Q. Isolation and characterisation of collagens from the skin, scale and bone of deep-sea redfish (Sebastes mentella). Food Chemistry. 2008 May;108(2): 616–23. DOI:10.1016/j.foodchem.2007.11.017.
  • Zhang M, WenTao L, GuoYing L. Isolation and characterisation of collagens from the skin of largefin longbarbel catfish (Mystus macropterus). Food Chemistry. 2009 Aug; 115(3):826-31. DOI: 10.1016/j.foodchem.2009.01.006.
  • Kittiphattanabawon P, Benjakul S, Visessanguan W, Shahidi F. Isolation and properties of acid- and pepsin-soluble collagen from the skin of blacktip shark (Carcharhinus limbatus). European Food Research and Technology. 2010
  • Jan;230:475–483. DOI: 10.1007/s00217-009-1191-0.
  • Huang Y-R, Shiau C-Y, Chen H-H, Huang B-C. Isolation and characterization of acid and pepsin-solubilized collagens from the skin of balloon fish (Diodon holocanthus). Food Hydrocolloids. 2011 Febr;25:1507-13. DOI:10.1016/j.foodhyd.2011.02.011.
  • Bonner P. L. R. Protein Purification (The Basics). Biochemistry and Molecular Biology Education. 2008;36(2):170. DOI: 10.1002/bmb.20150.
  • Vojdani F. Solubility. In Methods of Testing Protein Functionality. G.M. Hall ed. Blackie Academic and Professional. London. U.K. 1996; 11-46.
  • Zhang M, Liu W, Li G. Isolation and characterisation of collagens from the skin of largefin longbarbel catfish (Mystus macropterus). Food Chemistry. 2009 Aug;115:826-31. DOI: 10.1016/j.foodchem.2009.01.006
  • Song E, Kim S. Y, Chunc T, Byunc H-J, Leea YM. Collagen scaffolds derived from a marine source and their biocompatibility. Biomaterials. 2006 May;27(15):2951-61. DOI:10.1016/j.biomaterials.2006.01.015.
  • Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar D. S, Polymeric Scaffolds in Tissue Engineering Application: A Review. International Journal of Polymer Science, 2011 July;2011:1-19. DOI:10.1155/2011/290602.
Journal Section Articles
Authors

Author: Nimet Bölgen

Author: Zeynep Çetinkaya

Author: Didem Demir

Dates

Publication Date: January 8, 2017

Bibtex @ { jotcsa287274, journal = {Journal of the Turkish Chemical Society Section A: Chemistry}, issn = {}, eissn = {2149-0120}, address = {Turkish Chemical Society}, year = {2017}, volume = {3}, pages = {329 - 348}, doi = {10.18596/jotcsa.25993}, title = {FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION}, key = {cite}, author = {Bölgen, Nimet and Çetinkaya, Zeynep and Demir, Didem} }
APA Bölgen, N , Çetinkaya, Z , Demir, D . (2017). FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION. Journal of the Turkish Chemical Society Section A: Chemistry, 3 (3), 329-348. DOI: 10.18596/jotcsa.25993
MLA Bölgen, N , Çetinkaya, Z , Demir, D . "FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION". Journal of the Turkish Chemical Society Section A: Chemistry 3 (2017): 329-348 <http://dergipark.org.tr/jotcsa/issue/27290/287274?publisher=turchemsoc?publisher=turchemsoc>
Chicago Bölgen, N , Çetinkaya, Z , Demir, D . "FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION". Journal of the Turkish Chemical Society Section A: Chemistry 3 (2017): 329-348
RIS TY - JOUR T1 - FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION AU - Nimet Bölgen , Zeynep Çetinkaya , Didem Demir Y1 - 2017 PY - 2017 N1 - doi: 10.18596/jotcsa.25993 DO - 10.18596/jotcsa.25993 T2 - Journal of the Turkish Chemical Society Section A: Chemistry JF - Journal JO - JOR SP - 329 EP - 348 VL - 3 IS - 3 SN - -2149-0120 M3 - doi: 10.18596/jotcsa.25993 UR - https://doi.org/10.18596/jotcsa.25993 Y2 - 2019 ER -
EndNote %0 Journal of the Turkish Chemical Society Section A: Chemistry FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION %A Nimet Bölgen , Zeynep Çetinkaya , Didem Demir %T FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION %D 2017 %J Journal of the Turkish Chemical Society Section A: Chemistry %P -2149-0120 %V 3 %N 3 %R doi: 10.18596/jotcsa.25993 %U 10.18596/jotcsa.25993
ISNAD Bölgen, Nimet , Çetinkaya, Zeynep , Demir, Didem . "FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION". Journal of the Turkish Chemical Society Section A: Chemistry 3 / 3 (January 2017): 329-348. https://doi.org/10.18596/jotcsa.25993
AMA Bölgen N , Çetinkaya Z , Demir D . FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION. JOTCSA. 2017; 3(3): 329-348.
Vancouver Bölgen N , Çetinkaya Z , Demir D . FISH SKIN ISOLATED COLLAGEN CRYOGELS FOR TISSUE ENGINEERING APPLICATIONS: PURIFICATION, SYNTHESIS AND CHARACTERIZATION. Journal of the Turkish Chemical Society Section A: Chemistry. 2017; 3(3): 348-329.