Year 2019, Volume 9, Issue 1, Pages 144 - 151 2019-06-30

Investigation of Antimicrobial and Enzyme Production Capacities of Actinobacteria Isolated from Yıldız Lake Sediment
Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması

Kadriye ÖZCAN [1]

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In this study, actinobacteria was isolated from Yıldız Lake (Gümüşhane) sediment and the isolates were examined for antimicrobial activity and enzyme production capacities (amylase, lipase, protease, pectinase, cellulase). Screening was carried out on petri dishes containing suitable media. SCA medium was used for isolation and nistatin and nalidixic acid were added. For the detection of antimicrobial activity cross-streak method and C. tropicalis, C. albicans, E. coli, P. aeruginosa, MRSA, E. faecium, E. fecalis standard strains were used. As a result, 55% of the isolates showed antimicrobial activity agains to at least one test microorganism while 66.6% were able to produce at least one enzyme. However, 22.2% of the isolates were not capable of producing either antimicrobial activity or enzyme. This study was the first study on isolation of actinobacteria from Yıldız Lake and the biological activity of isolates. As a results, it is expected that the actinobacteria obtained from this sediment may be the source for industrial and pharmacological studies, but further studies are needed for the isolation and characterization of the active compounds.

Bu çalışmada, Yıldız Gölü (Gümüşhane) sedimentinden aktinobakteri izolasyonu gerçekleştirilmiş ve izolatlar antimikrobiyal aktivite ve enzim üretme kapasiteleri (amilaz, lipaz, proteaz, pektinaz, selülaz) bakımından incelenmiştir. Tarama, uygun besiyeriler içeren petrilerde gerçekleştirilmiştir. İzolasyon için SCA besiyeri kullanılmış ve besiyeriye nistatin ve nalidiksik asit ilave edilmiştir. Antimikrobiyal aktivite tespiti için, çapraz çizgi ekim yöntemi ve C. tropicalis, C. albicans, E. coli, P. aeruginosa, MRSA, E. faecium, E. fecalis standart suşları tercih edilmiştir. Sonuç olarak izolatların  %55’i en az bir test mikroorganizmasına karşı antimikrobiyal aktivite gösterirken %66.6’sının ise en az bir enzimi üretebildiği belirlenmiştir. Bununla birlikte izolatların %22.2’si ne antimikrobiyal aktivite ne de enzim üretme yeteneğine sahip bulunmuştur.  Bu çalışma Yıldız Gölü’nden aktinobakteri izolasyonu ve izolatların biyolojik aktivitesi üzerine gerçekleştirilen ilk araştırmadır. Sonuç olarak, elde edilen aktinobakterilerin endüstriyel ve farmakolojik çalışmalar için kaynak olabileceği öngörülmekle birlikte aktif bileşiklerin izolasyonu ve karakterizasyonu için ileri çalışmalara ihtiyaç duyulmaktadır

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Primary Language tr
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0002-4913-6035
Author: Kadriye ÖZCAN (Primary Author)
Institution: GİRESUN ÜNİVERSİTESİ
Country: Turkey


Dates

Publication Date: June 30, 2019

Bibtex @research article { kfbd563428, journal = {Karadeniz Fen Bilimleri Dergisi}, issn = {1309-4726}, eissn = {2564-7377}, address = {Giresun University}, year = {2019}, volume = {9}, pages = {144 - 151}, doi = {10.31466/kfbd.563428}, title = {Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması}, key = {cite}, author = {ÖZCAN, Kadriye} }
APA ÖZCAN, K . (2019). Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması. Karadeniz Fen Bilimleri Dergisi, 9 (1), 144-151. DOI: 10.31466/kfbd.563428
MLA ÖZCAN, K . "Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması". Karadeniz Fen Bilimleri Dergisi 9 (2019): 144-151 <http://dergipark.org.tr/kfbd/issue/45378/563428>
Chicago ÖZCAN, K . "Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması". Karadeniz Fen Bilimleri Dergisi 9 (2019): 144-151
RIS TY - JOUR T1 - Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması AU - Kadriye ÖZCAN Y1 - 2019 PY - 2019 N1 - doi: 10.31466/kfbd.563428 DO - 10.31466/kfbd.563428 T2 - Karadeniz Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 144 EP - 151 VL - 9 IS - 1 SN - 1309-4726-2564-7377 M3 - doi: 10.31466/kfbd.563428 UR - https://doi.org/10.31466/kfbd.563428 Y2 - 2019 ER -
EndNote %0 The Black Sea Journal of Sciences Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması %A Kadriye ÖZCAN %T Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması %D 2019 %J Karadeniz Fen Bilimleri Dergisi %P 1309-4726-2564-7377 %V 9 %N 1 %R doi: 10.31466/kfbd.563428 %U 10.31466/kfbd.563428
ISNAD ÖZCAN, Kadriye . "Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması". Karadeniz Fen Bilimleri Dergisi 9 / 1 (June 2019): 144-151. https://doi.org/10.31466/kfbd.563428
AMA ÖZCAN K . Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması. KFBD. 2019; 9(1): 144-151.
Vancouver ÖZCAN K . Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması. Karadeniz Fen Bilimleri Dergisi. 2019; 9(1): 151-144.