Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasound on Orthodontic Tooth Movement

Yıl 2019, Cilt: 11 Sayı: 1, 119 - 125, 25.03.2019

Mine Geçgelen Cesur Tuna Onal Mehmet Dincer Bilgin Fevziye Burcu Sirin Sevinc Inan Ergun Cem Koken Afra Alkan Gokhan Cesur

https://doi.org/10.18521/ktd.510808

Cited By: 3

Öz

Objective: The aim of this study is to investigate the
effects of low intensity pulsed ultrasound on orthodontic tooth movement in
rats.

Methods:
For this study,
40 12-week-old adult male Wistar albino rats from the Animal Laboratory at
Adnan Menderes University, Faculty of Medicine, were used. Rats were divided into four groups of ten. Group 1 was
the untreated control group. In group 2, an orthodontic spring was used to move
teeth. In groups 3 and 4, orthodontic treatment was combined with low intensity pulsed ultrasound at 16
J/cm² or 48 J/cm², respectively, for 14 days. Tooth
movement was measured on day 14. Serum bone alkaline phosphatase (BALP) and
C-telopeptide of type I collagen (CTX-I) levels were analyzed biochemically.
The number of osteoclasts, osteoblasts and inflammatory cells, capillary
density and new bone formation was determined histologically. Receptor activator
of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG), vascular
endothelial growth factor (VGEF) and transforming growth factor-β (TGF-β) were
assessed using immunohistochemical staining.

Results:
BALP and CTX-I levels in group 4 were significantly higher than for group 1.
Tooth movement and the number of osteoclasts, inflammatory cells, and capillary
density in group 4 were significantly greater than for group 2. The intensity
levels of RANKL and OPG in group 4 were significantly greater than for group 2.

Conclusion: Ultrasound is noninvasive application and a
promising therapy for accelerating bone remodeling during orthodontic tooth
movement.

Anahtar Kelimeler

Orthodontic treatment, low intensity pulsed ultrasound, tooth movement

Kaynakça

• 1) Huang H, Williams RC, Kyrkanides S. Accelerated orthodontic tooth movement: molecular mechanisms. Am J Orthod Dentofacial Orthop 2014;146:620-32.
• 2) Miresmaeili A, Mollaei N, Azar R, et al. Effect of dietary vitamin C on orthodontic tooth movement in rats. J Dent (Tehran) 2015;12:409-13.
• 3) Qamruddin I, Alam MK, Khamis MF, et al. Minimally invasive techniques to accelerate the orthodontic tooth movement: a systemic review of animal studies. Biomed Res Int 2015; 608530.
• 4) Gulec A¸ Bakkalbası BC, Cumbul A, et al. Effects of local platelet-rich plasma injection on the rate of orthodontic tooth movement in a rat model: A histomorphometric study. Am J Orthod Dentofac Orthop 2017;151:92-104.
• 5) Haugen S, Aasarød KM, Stunes AK, et al. Adiponectin prevents orthodontic tooth movement in rats. Arch Oral Biol 2017;83:304-11.
• 6) Brunet MD, Araujo CM, Johann AC, et al. Effects of zoledronic acid on orthodontic tooth movement in rats. Braz Dent J 2016;27:515-23.
• 7) Aghili H, Yassaei S, Zahir ST, et al. Effect of methylphenidate on tooth movement and histological features in rats. J Clin Diagn Res 2017;11:ZF01-05.
• 8) Kawakami M, Takano-Yamamoto T. Local injection of 1,25-dihydroxyvitamin D3 enhanced bone formation for tooth stabilization after experimental tooth movements in rats. J Bone Miner Metab 2004;22:541-6.
• 9) Soma S, Iwamoto M, Higuchi Y, et al. Effects of continuous infusion of PTH on experimental tooth movement in rats. J Bone Miner Res 1999;14:546-54.
• 10) Yamasaki K, Shibata Y, Fukuhara T. The effect of prostaglandins on experimental tooth movement in monkeys (Macacafuscata). J Dent Res 1982;61:1444-6.
• 11) Kobayashi Y, Takagi H, Sakai H, et al. Effects of local administration of osteocalcin on experimental tooth movement. Angle Orthod 1998;68: 259-66.
• 12) Chen Y, Wang XX, Zhao BJ, et al. Effects of icariin on orthodontic tooth movement in rats. Int J Clin Exp Med 2015;15:8608-16.
• 13) Tsuka Y, Fujita T, Shirakura M, et al. Effects of neodymium-dopedyttrium aluminium garnet (Nd:YAG) laser irradiation on bone metabolism during tooth movement. J Lasers Med 2016;7:40-4.
• 14) Milligan M, Arudchelvan Y, Gong SG. Effects of two wattages of low-level laser therapy on orthodontic tooth movement. Arch Oral Biol 2017;80:62-8.
• 15) Altan BA, Sokucu O, Ozkut MM, et al. Metrical and histological investigation of the effects of low-level laser therapy on orthodontic tooth movement. Lasers Med Sci 2012;27:131-40.
• 16) Feres MFN, Kucharski C, Diar-Bakirly S, et al. Effect of low-intensity pulsed ultrasound on the activity of osteoclasts: an in vitro study. Arch Oral Biol 2016;70:73-8.
• 17) Dahhas FY, El-Bialy T, Afify AR, et al. Effects of low-intensity pulsed ultrasound on orthodontic tooth movement and orthodontically induced inflammatory root resorption in ovariectomized osteoporotic rats. Ultrasound Med Biol 2016;42:808-14.
• 18) Xue H, Zheng J, Cui Z, et al. Low-intensity pulsed ultrasound accelerates tooth movement via activation of the BMP-2 signaling pathway. Plos One 2013;8:e68926.
• 19) El-Bialy T, El-Shamy I, Graber TM. Repair of orthodontically induced root resorption by ultrasound in humans. Am J Orthod Dentofac Orthop 2004;126:186-93.
• 20) El-Bialy T, Janadas A, Albaghdadi T. Nonsurgical treatment of hemifacial microsomia by therapeutic ultrasound and hybrid functional appliance. Open Access J Clin Trials 2010;2,29-36.
• 21) Toy E, Oztürk F, Altindiş S, et al. Effects of low-intensity pulsed ultrasound on bone formation after the expansion of the inter-premaxillary suture in rats: a histologic and immunohistochemical study. Aust Orthod J 2014;30:176-183.
• 22) Watson T. Ultrasound in contemporary physiotherapy practice. Ultrasonics 2008;48:321-29.
• 23) Khanna A, Nelmes RT, Gougoulias N, et al. The effects of LIPUS on soft-tissue healing: a review of literature. Br Med Bull 2009;89:169-82.
• 24) terHaar G. Therapeutic ultrasound. Eur J Ultrasound 1999; 9:3-9.
• 25) Robertson VJ, Baker KG. A review of therapeutic ultrasound: effectiveness studies. Phys Ther. 2001;81:1339-50.
• 26) Gerdhem P, Ivaska KK, Alatalo SL, et al. Biochemical markers of bone metabolism and prediction of fracture in elderly women. J Bone Miner Res 2004;19:386-93.
• 27) Reher P, Elbeshier EI, Harvey W,et al. The stimulation of bone formation in vitro by therapeutic ultrasound. Ultrasound Med Biol 1997;23:1251-8.
• 28) Reher P, Harris M, Whiteman M, et al. Ultrasound stimulates nitric oxide and prostaglandin E2 production by human osteoblast. Bone 2002;3:236-41.
• 29) Suzuki A, Takayama T, Suzuki N, et al. Daily low-intensity pulsed ultrasound mediated osteogenic differentiation in rat osteoblasts. Acta Biochim Biophys Sin (Shanghai) 2009;41:108-15.
• 30) Kantarci A, Will L, Yen S (eds). Tooth Movement. Front Oral Biol 2016, Basel, Karger, 9-16.
• 31) Borsje MA, Ren Y, de Haan-Visser HW, et al. Comparison of low-intensity pulsed ultrasound and pulsed electromagnetic field treatments on OPG and RANKL expression in human osteoblast-like cells. Angle Orthod 2010;80:498-503.
• 32) Dalla-Bona DA, Tanaka E, Inubushi T, et al. Cementoblast response to low- and high-intensity ultrasound. Arch Oral Biol 2008;53,318-23.