Year 2019, Volume 32, Issue 1, Pages 27 - 32 2019-01-29

Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats
Deneysel parsiyel splenektomi modelinde sıçanlarda algan hemostatik ajanın kanama kontrolünde etkinliğinin araştırılması

Ahmet MIDI [1] , Husamettin EKICI [2] , Ali KUMANDAS [3] , Omercan DURMUS [4] , Buse BODIC [5] , Mehmet TIRYAKI [6] , Mehmet Sabri BALİK [7] , Erdem YESILADA [8]

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Objective: Algan hemostatic agent (AHA) is a plant-based
hemostatic agent produced in Turkey. Although, there is a great
improvement in the hemostatic technologies, more effective
hemostatic products are required to be produced. The aim of
this study was to demonstrate the efficacy of AHA in a partial
splenectomy model in rats. In addition, in this model, postoperative
abdominal adhesion was evaluated.
Materials and Methods: In this study 5-7 weeks old 64
rats were used. Rats were randomly divided into 8 groups, each
consisting of eight rats (4 groups heparinized and 4 groups nonheparinized).
Experimental splenectomy was performed and the
gauze impregnated with saline was applied to the control group for
the hemorrhage control, the gauze impregnated with liquid AHA,
gel and powder form of AHA, was applied to the experimental
groups.
Results: The time to reach complete homeostasis was
significantly shorter in all AHA groups compared to the control
group. The powder and the gel forms of AHA stopped the bleeding
in heparinized and non-heparinized groups in 1 second. The AHA
fluid (sponge) form stopped the bleeding in the first application
in the control group less than 10 seconds and the second time
application was not necessary. The bleeding was able to be
controlled in the heparinized control group (saline impregnated
sponge) by 55 seconds and in the non-heparinized control group
by 38 seconds.
Conclusion: This study showed that AHA is a highly effective
hemostatic agent, which would be beneficial in controlling
hemorrhage.
Amaç: Algan hemostatik ajan (AHA), Türkiye’de üretilen bitki
bazlı hemostatik bir maddedir. Hemostatik teknolojilerdeki
büyük gelişmelere rağmen, kanamanın kontrolünde kullanılmak
üzere daha etkili hemostatik ürünlerin üretilmesi gerekmektedir.
Bu çalışmanın amacı, AHA’nın sıçanlarda parsiyel splenektomi
modelindeki etkinliğini göstermektir. Ayrıca bu modelde batın
yapışıklığı değerlendirilmiştir.
Gereçler ve Yöntemler: Bu çalışmada 5-7 haftalık 64 sıçan
kullanıldı. Sıçanların her biri sekiz sıçandan oluşan 8 gruba
ayrıldı (heparinize edilmiş 4 grup ve heparinize edilmemiş 4
grup). Deneysel splenektomi yapıldı ve kontrol grubuna hemoraji
kontrolü için serum fizyolojik emdirilmiş gazlı bez uygulandı,
deney gruplarına AHA sıvı emdirilmiş gazlı bez, jel ve toz formu
uygulandı.
Bulgular: Komple hemostaza ulaşma süresi, tüm AHA
gruplarında kontrol grubuna kıyasla anlamlı olarak daha kısa idi.
AHA’nın toz ve jel formları heparinize ve heparinize olmayan
gruplarda 1 saniyede kanamayı durdurdu. AHA sıvı (sünger)
formu, kontrol grubunda ilk uygulamada tüm sıçanlarda kanamayı
durdurdu (10 saniyenin altında). Kanama, heparinize edilmiş
kontrol grubunda (serum fizyolojik emdirilmiş gazlı bez) ortalama
55 saniyede ve heparinize olmayan kontrol grubunda 38 saniyede
kontrol edilebildi.
Sonuç: Bu çalışma, AHA’nın hemorajinin kontrol edilmesinde
yararlı olabilecek oldukça etkili bir hemostatik ajan olduğunu
göstermiştir.
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Primary Language en
Subjects Medicine
Journal Section Articles
Authors

Author: Ahmet MIDI (Primary Author)

Author: Husamettin EKICI

Author: Ali KUMANDAS

Author: Omercan DURMUS

Author: Buse BODIC

Author: Mehmet TIRYAKI

Author: Mehmet Sabri BALİK

Author: Erdem YESILADA

Dates

Publication Date: January 29, 2019

Bibtex @research article { marumj518821, journal = {Marmara Medical Journal}, issn = {1019-1941}, eissn = {1309-9469}, address = {Marmara University}, year = {2019}, volume = {32}, pages = {27 - 32}, doi = {10.5472/marumj.518821}, title = {Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats}, key = {cite}, author = {MIDI, Ahmet and EKICI, Husamettin and KUMANDAS, Ali and DURMUS, Omercan and BODIC, Buse and TIRYAKI, Mehmet and BALİK, Mehmet Sabri and YESILADA, Erdem} }
APA MIDI, A , EKICI, H , KUMANDAS, A , DURMUS, O , BODIC, B , TIRYAKI, M , BALİK, M , YESILADA, E . (2019). Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats. Marmara Medical Journal, 32 (1), 27-32. DOI: 10.5472/marumj.518821
MLA MIDI, A , EKICI, H , KUMANDAS, A , DURMUS, O , BODIC, B , TIRYAKI, M , BALİK, M , YESILADA, E . "Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats". Marmara Medical Journal 32 (2019): 27-32 <http://dergipark.org.tr/marumj/issue/42918/518821>
Chicago MIDI, A , EKICI, H , KUMANDAS, A , DURMUS, O , BODIC, B , TIRYAKI, M , BALİK, M , YESILADA, E . "Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats". Marmara Medical Journal 32 (2019): 27-32
RIS TY - JOUR T1 - Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats AU - Ahmet MIDI , Husamettin EKICI , Ali KUMANDAS , Omercan DURMUS , Buse BODIC , Mehmet TIRYAKI , Mehmet Sabri BALİK , Erdem YESILADA Y1 - 2019 PY - 2019 N1 - doi: 10.5472/marumj.518821 DO - 10.5472/marumj.518821 T2 - Marmara Medical Journal JF - Journal JO - JOR SP - 27 EP - 32 VL - 32 IS - 1 SN - 1019-1941-1309-9469 M3 - doi: 10.5472/marumj.518821 UR - https://doi.org/10.5472/marumj.518821 Y2 - 2018 ER -
EndNote %0 Marmara Medical Journal Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats %A Ahmet MIDI , Husamettin EKICI , Ali KUMANDAS , Omercan DURMUS , Buse BODIC , Mehmet TIRYAKI , Mehmet Sabri BALİK , Erdem YESILADA %T Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats %D 2019 %J Marmara Medical Journal %P 1019-1941-1309-9469 %V 32 %N 1 %R doi: 10.5472/marumj.518821 %U 10.5472/marumj.518821
ISNAD MIDI, Ahmet , EKICI, Husamettin , KUMANDAS, Ali , DURMUS, Omercan , BODIC, Buse , TIRYAKI, Mehmet , BALİK, Mehmet Sabri , YESILADA, Erdem . "Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats". Marmara Medical Journal 32 / 1 (January 2019): 27-32. https://doi.org/10.5472/marumj.518821
AMA MIDI A , EKICI H , KUMANDAS A , DURMUS O , BODIC B , TIRYAKI M , BALİK M , YESILADA E . Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats. Marmara Med J. 2019; 32(1): 27-32.
Vancouver MIDI A , EKICI H , KUMANDAS A , DURMUS O , BODIC B , TIRYAKI M , BALİK M , YESILADA E . Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats. Marmara Medical Journal. 2019; 32(1): 32-27.