Year 2019, Volume 32, Issue 1, Pages 38 - 43 2019-01-29

Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line
İnsan kronik miyeloid lösemi K562 hücrelerinde karbakolün apoptoza etkisi

Banu AYDIN [1] , Aysın TULUNAY [2] , Emel EKŞİOĞLU-DEMİRALP [3] , Beki KAN [4] , Hulya CABADAK [5]

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Objectives: Muscarinic receptors mediate diverse actions of
acetylcholine in the central nervous system and in non-nervous
tissues innervated by the parasympathetic nervous system.
Our study aims to evaluate the potential association of the
M3 muscarinic receptor with K562 cell proliferation and death.
Materials and Methods: Cell proliferation was evaluated
by bromodeoxyuridine (BrDU) incorporation. To show early,
late apoptosis and cell death, cells were labelled with Annexin
V, propidium iodide (PI) and analyzed by flow cytometry. Nuclear
extracellular signal-regulated kinase (ERK/pERK) expression
was measured by western blot analysis.
Results: Treatment with carbachol (CCh) for 48h decreased cell
number. Exposing K562 cells to CCh for 24h decreased the number of
early apoptotic cells but did not change the number of late apoptotic and
necrotic cells. CCh treatment for 48h increased the number of necrotic
cells, but decreased the number of early and late apoptotic cells. In
response to CCh, nuclear ERK expression was increased and this effect
was reversed by 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide
(4DAMP). Nuclear pERK expression was decreased in CCh treated
cells, 4DAMP did not reverse the effect.
Conclusion: Our data suggest that cholinergic agonist CCh
affects cell proliferation in K562 cells not only through muscarinic
receptors but also through other cholinergic receptors.
Amaç: Muskarinik reseptörler merkezi sinir sisteminde
asetilkolinin çeşitli etkilerine aracılık ettiği gibi, parasempatik sinir
sistemi ile etkileşen sinirsel olmayan dokulara da aracılık ederler.
Çalışmamızda, M3 muskarinik reseptör alttipinin K562 kanser
hücre çoğalması ve ölümündeki rolünü belirlemeye çalıştık.
Gereçler ve Yöntemler: Hücre çoğalması bromodeoksiüridin
(BrDU) yöntemi ile belirlenmiştir. Hücre ölümü, erken ve geç
apoptoz Anneksin V ve propidyum iodid (PI) varlığında akış
sitometrisi yöntemi ile gösterilmiştir. Nuklear dış sinyal düzenleyici
kinaz (ERK/fosfo-ERK) ekspresyonu western emdirimi yöntemi
ile belirlenmiştir.
Bulgular: Çalışmamızda 48 saat karbakol(CCh) ile muamele
edilen K562 hücre sayısında azalma belirlenmiştir. Hücreler
24 saat CCh ile muamele edildiklerinde erken apoptotik hücre
sayısında azalma gözlemlenirken geç apoptoz ve nekrotik hücre
sayısında değişim olmamıştır. Bununla birlikte, 48 saat boyunca
CCh ile muamele olan hücrelerde, erken ve geç apoptotik hücre
sayısı azalırken, nekrotik hücrelerin sayısı artmıştır. CCh ile
muamele edilen hücrelerde nüklear ERK ekspresyonu artarken
bu etki 1,1-dimetil-4-difenilasetoksipiperidinium iodid (4DAMP)
ile geri çevrilmiştir. Aynı koşullarda CCh ile muamele edilen
hücrelerde nuklear pERK ekspresyonu azalmış, bu etki 4DAMP
ile geri çevrilmemiştir.
Sonuç: Bulgularımız, K562 hücre proliferasyonundaki
kolinerjik etkinin yalnızca muskarinik mekanizma ile değil
diğer kolinerjik reseptörlerin de katkısıyla gerçekleştiğini
düşündürmektedir.
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Primary Language en
Subjects Medicine
Journal Section Articles
Authors

Author: Banu AYDIN (Primary Author)

Author: Aysın TULUNAY

Author: Emel EKŞİOĞLU-DEMİRALP

Author: Beki KAN

Author: Hulya CABADAK

Dates

Publication Date: January 29, 2019

Bibtex @research article { marumj518983, journal = {Marmara Medical Journal}, issn = {1019-1941}, eissn = {1309-9469}, address = {Marmara University}, year = {2019}, volume = {32}, pages = {38 - 43}, doi = {10.5472/marumj.518983}, title = {Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line}, key = {cite}, author = {AYDIN, Banu and TULUNAY, Aysın and EKŞİOĞLU-DEMİRALP, Emel and KAN, Beki and CABADAK, Hulya} }
APA AYDIN, B , TULUNAY, A , EKŞİOĞLU-DEMİRALP, E , KAN, B , CABADAK, H . (2019). Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line. Marmara Medical Journal, 32 (1), 38-43. DOI: 10.5472/marumj.518983
MLA AYDIN, B , TULUNAY, A , EKŞİOĞLU-DEMİRALP, E , KAN, B , CABADAK, H . "Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line". Marmara Medical Journal 32 (2019): 38-43 <http://dergipark.org.tr/marumj/issue/42918/518983>
Chicago AYDIN, B , TULUNAY, A , EKŞİOĞLU-DEMİRALP, E , KAN, B , CABADAK, H . "Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line". Marmara Medical Journal 32 (2019): 38-43
RIS TY - JOUR T1 - Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line AU - Banu AYDIN , Aysın TULUNAY , Emel EKŞİOĞLU-DEMİRALP , Beki KAN , Hulya CABADAK Y1 - 2019 PY - 2019 N1 - doi: 10.5472/marumj.518983 DO - 10.5472/marumj.518983 T2 - Marmara Medical Journal JF - Journal JO - JOR SP - 38 EP - 43 VL - 32 IS - 1 SN - 1019-1941-1309-9469 M3 - doi: 10.5472/marumj.518983 UR - https://doi.org/10.5472/marumj.518983 Y2 - 2018 ER -
EndNote %0 Marmara Medical Journal Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line %A Banu AYDIN , Aysın TULUNAY , Emel EKŞİOĞLU-DEMİRALP , Beki KAN , Hulya CABADAK %T Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line %D 2019 %J Marmara Medical Journal %P 1019-1941-1309-9469 %V 32 %N 1 %R doi: 10.5472/marumj.518983 %U 10.5472/marumj.518983
ISNAD AYDIN, Banu , TULUNAY, Aysın , EKŞİOĞLU-DEMİRALP, Emel , KAN, Beki , CABADAK, Hulya . "Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line". Marmara Medical Journal 32 / 1 (January 2019): 38-43. https://doi.org/10.5472/marumj.518983
AMA AYDIN B , TULUNAY A , EKŞİOĞLU-DEMİRALP E , KAN B , CABADAK H . Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line. Marmara Med J. 2019; 32(1): 38-43.
Vancouver AYDIN B , TULUNAY A , EKŞİOĞLU-DEMİRALP E , KAN B , CABADAK H . Effects of carbachol on apoptosis in human chronic myelogenous leukemic K562 cell line. Marmara Medical Journal. 2019; 32(1): 43-38.