Year 2018, Volume 8, Issue 3, Pages 190 - 195 2018-09-28

Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes

Turgut Taşkın [1] , Tugce Yesil Devecioglu [2] , İsmail Şenkardeş [3]

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Objective: Terfezia claveryi is a dark brown color truffle and has been used

extensively in human diet in Turkey. Therefore; it is an important issue to determine

their biological activities and toxicity. This study aims to investigate the antioxidant and

anti-urease effects of T. claveryi methanol extracts and to evaluate the genotoxicity

of each extract.

Methods: The antioxidant activities of methanol extracts were examined by

2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-

sulfonate (ABTS) and ferric reducing/antioxidant power (FRAP) methods. The

amount of total phenolic compounds contained in the extracts was determined using

the Folin-Ciocalteu reagent (FCR) method. Anti-urease activities of methanol extracts

were evaluated by indophenol method. In addition, potential DNA damaging effects of

T. claveryi extracts on human lymphocytes were determined by the alkaline comet

assay.

Results: UM extract exhibited higher DPPH radical scavenging activity than MM

extract [the concentration of the substance which provides the inhibition of 50% of the

radical (IC50):0.77±0.01 mg/mL, 1.17±0.02 mg/mL, respectively, p<0.0001] however,

lower activity than standard (p<0.0001). Both extracts in different concentrations of

T. claveryi caused DNA damage (p<0.0001) compared with solvent control DMSO.

Also, total comet scores was higher in cells which had been incubated with MM

extract (p<0.05). UM extract exhibited higher ferric reducing and anti-urease activity

than MM extract but not found statistically different; also activities of both extract were

found lower than positive controls of each methods (p<0.0001).

Conclusion: T. claveryi methanol extracts induced genotoxicity on lymphocytes and

showed very low antioxidant activity compared to standards. This preliminary study

should be confirmed with further studies and in diferent cell lines.

T. claveryi, genotoxic, antioxidant, anti-urease, maceration, ultrasonic bath
  • Gadallah MGE, Ashoush IS. Value addition on nutritional and sensory properties of biscuit using desert truffle (Terfezia claveryi) Powder. Food Nutr Sci. 2016; 7: 1171-1181.
Primary Language en
Subjects Health Care Sciences and Services
Journal Section Articles
Authors

Author: Turgut Taşkın

Author: Tugce Yesil Devecioglu

Author: İsmail Şenkardeş

Dates

Publication Date: September 28, 2018

Bibtex @research article { marusbed465488, journal = {Clinical and Experimental Health Sciences}, issn = {}, eissn = {2459-1459}, address = {Marmara University}, year = {2018}, volume = {8}, pages = {190 - 195}, doi = {10.5152/clinexphealthsci.2017.651}, title = {Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes}, key = {cite}, author = {Taşkın, Turgut and Yesil Devecioglu, Tugce and Şenkardeş, İsmail} }
APA Taşkın, T , Yesil Devecioglu, T , Şenkardeş, İ . (2018). Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes. Clinical and Experimental Health Sciences, 8 (3), 190-195. DOI: 10.5152/clinexphealthsci.2017.651
MLA Taşkın, T , Yesil Devecioglu, T , Şenkardeş, İ . "Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes". Clinical and Experimental Health Sciences 8 (2018): 190-195 <http://dergipark.org.tr/marusbed/issue/39474/465488>
Chicago Taşkın, T , Yesil Devecioglu, T , Şenkardeş, İ . "Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes". Clinical and Experimental Health Sciences 8 (2018): 190-195
RIS TY - JOUR T1 - Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes AU - Turgut Taşkın , Tugce Yesil Devecioglu , İsmail Şenkardeş Y1 - 2018 PY - 2018 N1 - doi: 10.5152/clinexphealthsci.2017.651 DO - 10.5152/clinexphealthsci.2017.651 T2 - Clinical and Experimental Health Sciences JF - Journal JO - JOR SP - 190 EP - 195 VL - 8 IS - 3 SN - -2459-1459 M3 - doi: 10.5152/clinexphealthsci.2017.651 UR - https://doi.org/10.5152/clinexphealthsci.2017.651 Y2 - 2019 ER -
EndNote %0 Clinical and Experimental Health Sciences Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes %A Turgut Taşkın , Tugce Yesil Devecioglu , İsmail Şenkardeş %T Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes %D 2018 %J Clinical and Experimental Health Sciences %P -2459-1459 %V 8 %N 3 %R doi: 10.5152/clinexphealthsci.2017.651 %U 10.5152/clinexphealthsci.2017.651
ISNAD Taşkın, Turgut , Yesil Devecioglu, Tugce , Şenkardeş, İsmail . "Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes". Clinical and Experimental Health Sciences 8 / 3 (September 2018): 190-195. https://doi.org/10.5152/clinexphealthsci.2017.651
AMA Taşkın T , Yesil Devecioglu T , Şenkardeş İ . Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes. Clinical and Experimental Health Sciences. 2018; 8(3): 190-195.
Vancouver Taşkın T , Yesil Devecioglu T , Şenkardeş İ . Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes. Clinical and Experimental Health Sciences. 2018; 8(3): 195-190.