Year 2019, Volume 32, Issue 1, Pages 35 - 41 2019-04-01

Bor stresine maruz bırakılmış kanola bitkilerinde (Brassica napus ssp. oleifera L.) selenyum uygulaması sonrası ROS tepki genlerinin ifadelerinin belirlenmesi
Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment

Melahat Ozge ÖZEN [1] , Seyda KAYA [2] , Senem UYLAŞ [3] , Durmus ÇETİN [4] , Esin ARI [5] , M. Aydın AKBUDAK [6]

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Selenyumun (Se) çeşitli abiyotik stresler üzerindeki azaltıcı etkisi üzerine çalışmalar mevcut olmakla birlikte, B-stresi üzerindeki etkisi hakkında bilgi oldukça azdır. Bu nedenle, bu çalışmada B-stresine maruz bırakılmış kanola (Brassica napus ssp. oleifera L.) bitkileri üzerinde selenyumun etkilerinin ortaya konulması hedeflenmiştir. Ayrı ayrı (B veya Se) ve eş zamanlı (B + Se) uygulamaların belli başlı antioksidan enzimlerinin yapraklardaki gen ekspresyonu ve protein miktarları üzerine etkisi araştırılmıştır. Yapılan ayrı ayrı uygulamalar (litrede) 1.5 g B, 3 g B, 3 mg Se ve 6 mg Se'yi içerirken, eş zamanlı uygulamalar 1.5 g B + 3 mg Se, 1.5 g B + 6 mg Se, 3g B + 3 mg Se ve 3 g B + 6 mg Se içermektedir. Uygulamalar altında, APX, CAT, SOD, POD ve GR genlerinin çoğunlukla ifadeleri azalırken, APX (1.5 g B + 6 mg Se ve 3 g B + 6 mg Se), SOD (1.5 g B + 3 mg Se), CAT (3 g B, 3 mg Se ve 3 g B + 6 mg Se) ve POD (1.5 g B) gibi bazılarının ifadeleri ise artış göstermişlerdir. Bu çalışma Se' nin yararlı etkisinin ortamda herhangi bir stres etkeninin var olup-olmadığı ile ilişkili olabileceğini; aksi takdirde, Se' nin kendisinin doğrudan stres etkeni olarak antioksidan mekanizmayı indükleyebileceğini ortaya koymaktadır. Çalışmayla ayrıca, B ve Se uygulamalarının antioksidan gen ifadesi ve protein aktiviteleri üzerindeki eş zamanlı etkilerinin nasıl sonuçlandığı da gösterilmiştir.

Though mitigating effect of selenium (Se) on various abiotic stresses is apparent, the knowledge on B-stress is very scant. Therefore, this work attempted to reveal its effects on B-stressed canola (Brassica napus ssp. oleifera L.) plants. In the present study, foliar gene expression and amounts of major antioxidant enzymes were investigated under different concentrations of individual (B or Se) and combined (B + Se) applications. The individual applications (per L) included 1.5 g B, 3 g B, 3 mg Se and 6 mg Se, while the combined applications were 1.5 g B+3 mg Se, 1.5 g B+6 mg Se, 3 g B+3 mg Se and 3 g B+6 mg Se. Under the given treatments, APX, CAT, SOD, POD and GR genes were mostly downregulated while some also showed upregulation such as APX (1.5 g B+6 mg Se and 3 g B+6 mg Se), SOD (1.5 g B+3 mg Se), CAT (3 g B, 3 mg Se and 3 g B+6 mg Se) and POD (1.5 g B). Assaying also implicated that beneficial effect of Se might be associated with the presence of stressor; otherwise, Se itself might induce the antioxidant mechanism as stressor. This work provides the results of individual and combined effects of B and Se applications on antioxidant gene expressions and protein activities in canola.


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Primary Language en
Subjects Agricultural, Engineering
Journal Section Makaleler
Authors

Author: Melahat Ozge ÖZEN
Institution: AKDENIZ UNIVERSITY

Author: Seyda KAYA

Author: Senem UYLAŞ

Author: Durmus ÇETİN

Author: Esin ARI

Orcid: 0000-0002-1397-4678
Author: M. Aydın AKBUDAK (Primary Author)
Institution: AKDENİZ ÜNİVERSİTESİ
Country: Turkey


Bibtex @research article { mediterranean500611, journal = {Mediterranean Agricultural Sciences}, issn = {}, eissn = {2528-9675}, address = {Akdeniz University}, year = {2019}, volume = {32}, pages = {35 - 41}, doi = {10.29136/mediterranean.500611}, title = {Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment}, key = {cite}, author = {ÖZEN, Melahat Ozge and KAYA, Seyda and UYLAŞ, Senem and ÇETİN, Durmus and ARI, Esin and AKBUDAK, M. Aydın} }
APA ÖZEN, M , KAYA, S , UYLAŞ, S , ÇETİN, D , ARI, E , AKBUDAK, M . (2019). Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment. Mediterranean Agricultural Sciences, 32 (1), 35-41. DOI: 10.29136/mediterranean.500611
MLA ÖZEN, M , KAYA, S , UYLAŞ, S , ÇETİN, D , ARI, E , AKBUDAK, M . "Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment". Mediterranean Agricultural Sciences 32 (2019): 35-41 <http://dergipark.org.tr/mediterranean/issue/41736/500611>
Chicago ÖZEN, M , KAYA, S , UYLAŞ, S , ÇETİN, D , ARI, E , AKBUDAK, M . "Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment". Mediterranean Agricultural Sciences 32 (2019): 35-41
RIS TY - JOUR T1 - Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment AU - Melahat Ozge ÖZEN , Seyda KAYA , Senem UYLAŞ , Durmus ÇETİN , Esin ARI , M. Aydın AKBUDAK Y1 - 2019 PY - 2019 N1 - doi: 10.29136/mediterranean.500611 DO - 10.29136/mediterranean.500611 T2 - Mediterranean Agricultural Sciences JF - Journal JO - JOR SP - 35 EP - 41 VL - 32 IS - 1 SN - -2528-9675 M3 - doi: 10.29136/mediterranean.500611 UR - https://doi.org/10.29136/mediterranean.500611 Y2 - 2019 ER -
EndNote %0 Mediterranean Agricultural Sciences Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment %A Melahat Ozge ÖZEN , Seyda KAYA , Senem UYLAŞ , Durmus ÇETİN , Esin ARI , M. Aydın AKBUDAK %T Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment %D 2019 %J Mediterranean Agricultural Sciences %P -2528-9675 %V 32 %N 1 %R doi: 10.29136/mediterranean.500611 %U 10.29136/mediterranean.500611
ISNAD ÖZEN, Melahat Ozge , KAYA, Seyda , UYLAŞ, Senem , ÇETİN, Durmus , ARI, Esin , AKBUDAK, M. Aydın . "Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment". Mediterranean Agricultural Sciences 32 / 1 (April 2019): 35-41. https://doi.org/10.29136/mediterranean.500611