In vitro and in vivo assessment of the sensitivity of some tangerine mutants to Alternaria alternata pv. citri

Yıl 2019, Cilt: 32 Sayı: 2, 117 - 120, 01.08.2019

Ertuğrul Turgutoğlu İbrahim Baktır

https://doi.org/10.29136/mediterranean.452671

Cited By: 1

Öz

Alternaria brown spot disease caused by the Alternaria alternata pv. citri factor affects the leaves,
branches and fruits of many types of tangerines and their hybrids. This disease
has caused serious declines in the plantation areas of Minneola tangelo which
is widely produced in the Mediterranean Region while at the same time it has
hindered the expansion of the area of cultivation of late maturing tangerines
like Fortune. The isolate used in this study was obtained from a commercial
orchard of ‘Minneola tangelo’ in the province of Adana, Turkey. The sequence
analysis of the application of this isolates shows that a matchup of 99% was
achieved with isolate AHS-467.6 on the scale of the IFS primer. The aim of the
study was to identify tolerant genotypes from the mutants bred form the
‘Fortune’ tangerine which is known to be sensitive to the brown spot disease of
citrus. The sensitivity of individual mutants was tested by using leaves under in vitro conditions. The disease was
inoculated to tolerant mutant varieties under in vivo conditions and the results registered. The results of the
study showed that 9 mutant varieties among the 110 individuals under study were
found to be tolerant against the disease.

Anahtar Kelimeler

Citrus, Mutation, Alternaria brown spot disease, Fortune, Mandarin

In vitro ve in vivo koşullarında bazı mandarin mutantlarının Alternaria alternata pv. citri etmenine duyarlılıklarının değerlendirilmesi

Öz

Alternaria alternata pv. citri etmeninin neden
olduğu Alternaria kahverengi leke hastalığı birçok mandarin ve melezlerinin
yaprak, dal ve meyvelerini etkiler. Söz konusu hastalık Akdeniz bölgesinde yetiştiriciliği yapılan Minneola tanjelonun dikim
alanlarında ciddi azalmalara neden olmuş
ve ayrıca yetiştiricilik açısından geççi bir çeşit olan
Fortune gibi mandarin çeşitlerinin gelişimini engellemiştir. Çalışmada kullanılan
izolat Adana ilinde bulunan ticari ‘Minneola tanjelo’ bahçelerinden elde
edilmiştir. Bu izolat sekans analizi sonucunda ITS1 primerine göre %99 oranında
AHS-467-6 izolatı ile eşleşme sağlamıştır. Mutasyon ıslahı ile turunçgillerde
kahverengi leke hastalığına hassas olduğu bilinen ‘Fortune’ mandarini
mutantları arasından tolerant genotiplerin elde edilmesi hedeflenmiştir. In vitro koşullarda koparılmış yapraklar
kullanılarak mutant bireylerin hastalığa karşı hassasiyetleri belirlenmiştir.
Ayrıca, tolerant olarak bulunan mutantların fidanlarına in vivo koşullarda hastalık inokulasyonu yapılarak sonuçlar kontrol
edilmiştir. Çalışma sonucunda 111 mutant birey arasından 9 adet mutant birey
hastalığa karşı tolerant olarak bulunmuştur.

Anahtar Kelimeler

Turunçgil, Fortune, Mutasyon, Alternaria kahverengi leke hastalığı, Mandarin

Kaynakça

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