Investigation of volatile components and various in vitro biological activities of tilia tomentosa moench flowers

Yıl 2019, Cilt: 12 Sayı: 2, 220 - 229, 30.08.2019

Nihal Karakaş Mehmet Evren Okur Nurşah Öztunç Ayşe Esra Karadağ Şükran Kültür Betül Demirci

https://doi.org/10.26559/mersinsbd.505082

Cited By: 1

Öz

Aim: The aim of this study was to investigate the volatile components, total phenolic content, and in vitro antioxidant, antimicrobial and cell viability activities of Tilia tomentosa Moench. (Malvaceae) flower n-hexane extract. Methods: The dried plant material was powdered and extracted with n-hexane by maceration. The volatile components of the extract were investigated by Gas Chromatography / Mass Spectroscopy (GC/MS) and the total phenolic content was determined using the Folin-Ciocalteu method. The antioxidant capacity was tested in vitro DPPH and ABTS radical scavenging methods. Antimicrobial activity was investigated by microdilution method against microorganisms of Pseudomonas aeruginosa ATCC 10145, Escherichia coli NRLL B-3008, Staphylococcus aereus ATCC 6538, Helicobacter pylori ATCC 43504 and Mycobacterium smegmatis ATCC 25291. The effect on cell viability in vitro was determined by measuring the metabolic activity of the cells. Results: The ABTS and DPPH radical scavenging activity of T. tomentosa n-hexane extract was found as 1.94 and 1.03, respectively. In addition, the increased activity of the extract on in vitro cell viability was determined. It was observed that the extract was effective only against Staphylococcus aureus at a dose of 125 µg/mL. The major volatile component of the extract was camphor (17.7%). Conclusion: T. tomentosa n-hexane extract has a positive effect on cell viability, and it has a moderate level of antioxidant activity. For these reasons, the extract may be used as a cell regenerator.

Anahtar Kelimeler

Cell culture, Tilia tomentosa, antimicrobial, cell regenerator

Tilia tomentosa moench çiçeklerinin uçucu bileşenlerinin ve çeşitli in vitro biyolojik aktivitelerinin incelenmesi

Öz

Amaç: Tilia tomentosa Moench. (Malvaceae) bitkisinin
çiçeklerinden elde edilen n-hekzan
ekstresinin uçucu bileşenleri, toplam fenolik madde miktarı, in vitro antioksidan, antimikrobiyal ve hücre canlılığı
aktivitelerinin araştırılması amaçlanmıştır. Yöntem: Kurutulan bitki materyali toz edildikten sonra, n-hekzan kullanılarak maserasyon yöntemi
ile ekstre hazırlanmıştır. Uçucu bileşenleri Gaz Kromatografisi/Kütle
Spektroskopisi (GC/MS) ile araştırılmış, toplam fenolik madde miktarı
Folin-Ciocalteu yöntemi kullanılarak belirlenmiştir. Antioksidan kapasitesi
DPPH ve ABTS radikal süpürücü yöntemleri ile in vitro olarak test edilmiştir. Antimikrobiyal aktivitesi Pseudomonas aeruginosa ATCC 10145, Escherichia coli NRLL B-3008, Staphylococcus
aereus ATCC 6538, Helicobacter pylori ATCC 43504 ve Mycobacterium
smegmatis ATCC 25291 mikroorganizmalarına karşı mikrodilüsyon yöntemi
kullanılarak araştırılmıştır. In vitro hücre canlılığı üzerine etkisi
hücrelerin metabolik olarak aktif olma durumlarının ölçümü ile belirlenmiştir. Bulgular: T. tomentosa n-hekzan ekstresinin ABTS ve DPPH
radikal süpürücü etkisi sırasıyla 1.94 ve 1.03 olarak bunmuştur. Ek olarak,
ekstrenin in vitro hücre canlılığı
üzerinde azalan dozlarda artan aktivitesi tespit edilerek en yüksek aktivite 1
mg/mL dozunda gözlemlenmiştir. Ekstrenin denenen suşlardan yalnızca Staphylococcus aureus mikroorganizmasına
karşı 125 µg/mL dozda etkili olduğu
görülmüştür. Ayrıca major uçucu bileşeni %17.7 oranında kafur olarak tespit
edilmiştir. Sonuç: T. tomentosa n-hekzan ekstresinin, hücre canlılığı üzerinde olumlu etkiye sahip
olduğu ve orta düzeyde antioksidan etkinliğe sahip olduğundan dolayı hücre
yenileyici olarak kullanılabileceği düşünülmektedir.

Anahtar Kelimeler

Hücre kültürü, Tilia tomentosa, Antimikrobiyal, Hücre yenileyici, GC/MS

Kaynakça

• 1. Davis PH. Flora of Turkey and the East Aegean Islands. Vol 2. Edinburgh, Edinburgh University Press, 1967:421-424.
• 2. Güner A, Aslan S, Ekim T, Vural M, Babaç MT (eds.). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul, 2012:254-58.
• 3. Oral, D. Türkiyenin Doğal-Egzotik Ağaç ve Çalıları 2, Angiospermler (H-Z). Ed. Prof. Dr. Ünal Akkemik (Ġstanbul Ünv. Orman Fak. Orman Botaniği A.B.D.), OGM Genel Müdürlüğü Yayınları, Ankara, 2014:119-125.
• 4. Baytop T. Türkçe Bitki Adları Sözlüğü. Türk Dil Kurumu Yayınları, Ankara, 1994:78-79.
• 5. Baytop T. Türkiye’de Bitkiler ile Tedavi. 2nci baskı. Nobel Tıp Kitabevleri, Ankara, 1999:98-99.
• 6. Baser KHC, Tümen G, Malyer H, Kirimer N. Plants used for common cold in Turkey. Proc. ICEB, 2005:133-137.
• 7. Peev C, Dehelean C, Antal D, Feflea S, Olariu L, Toma C. Tilia tomentosa foliar bud extract: phytochemical analysis and dermatological testing. Studia Universitatis Vasile Goldiş Seria Ştiinţele Vieţii 2009;19:163-165.
• 8. Oniszczuk A and Podgórski R. Influence of different extraction methods on the quantification of selected flavonoids and phenolic acids from Tilia cordata inflorescence. Ind Crop Prod 2015; 76: 509–514.9. Yakovlev AI. Polysaccharides of the inflorescences of Tilia cordata. Chem Nat Comp 1985; 21 (1):114–115.
• 10. Kosakowska OK, Bączek K, Przybyła JL, Ejdysa M, Kuźma P, Obiedziński M, Węglarza Z. Intraspecific variability in the content of phenolic compounds, essential oil and mucilage of small-leaved lime (Tilia cordata Mill.) from Poland. Ind Crop Prod 2015; 78: 58–65.
• 11. Toker G. Türkiyede yetişen Tilia L. Türleri üzerinde Farmakognozik Araştırmalar. Doktora Tezi, Mekin Tanker, Yayınlanmamış Tez, Ankara, 1982.
• 12. Newall CA, Anderson LA, Phillipson JD. Herbal Medicines: A Guide for Health-Care Professionals, 1st press, London, The Pharmaceutical Press, 1996: 181.
• 13. Toker G, Aslan M, Yesilada E, Memisoglu M, Ito S. Comparative evaluation of the flavonoid content in officinal Tiliae flos and Turkish lime species for quality assessment. J Pharm Biomed Anal 2001; 26: 111-121.
• 14. Duke JA, Godwin MJB, Ducellier J, Duke PAK. Handbook of Medicinal Herbs, Boca Raton: Fla. CRC pres, 2002: 485-486.
• 15. Gruenwald J, Brendler T, Jaenicke C. PDR for Herbal Medicine, Herbal Monographs, (4th addition), USA: Thomson Press, 2007: 532-533.
• 16. Matsuda H, Ninomiya K, Shimoda H, Yoshikawa M. Hepatoprotective principles from the flowers of Tilia argentea (Linden): structure requirement of tiliroside and mechanism of action. Bioorg Med Chem, 2002; 10: 707-712.
• 17. Yesilada E, Üstün O, Sezik E, Takaishi Y, Ono Y, Honda G. Inhibitory effects of Turkish folk remedies on inflammatory cytokines; interleukin-1α, interleukin-1β and tumor necrosis factor-α. J Ethnopharmacol, 1997; 58: 59-73.
• 18. Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V. Comparison of antioxidant and antimicrobial activities of tilia (Tilia argentea Desf. ex DC), sage (Salvia triloba L.) and black tea (Camellia sinensis) extracts. J Agric Food Chem, 2000; 48: 5030-5034.
• 19. Toker G, Kupeli E, Memisoglu M, Yesilada E. Flavonoids with antinociceptive and anti-inflammatory activities from leaves of Tilia argentea (silver linden). J Ethnopharmacol, 2004; 95: 393-397.
• 20. Rop O, Mlcek J, Jurikova T, Neugebauerova J, Vabkova J. Edible Flowers—A New Promising Source of Mineral Elements in Human Nutrition. Molecules 2012; 17(6): 6672–83.
• 21. Blois MS.Antioxidant determinations by the use of a stable free radical. Nature 1958; 181:1199-1200.
• 22. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26: 1231–1237.23. Okur ME, Polat DC, Ozbek H, Yilmaz S, Yoltas A, Arslan R. Evaluation of the antidiabetic property of capparis ovata desf. Var. Paleastina zoh. Extracts using in vivo and in vitro approaches. Endocrine, Metab. Immune Disord. - Drug Targets 2018; 18: 489–501.
• 24. Spanos GA, Wrolstad RE. Influence of processing and storage on the phenolic composition of Thompson Seedless grape juice. J Agric Food Chem 1990; 38: 1565–1571.25. Okur ME, Ayla Ş, Polat DÇ, Günal MY, Yoltaş A, Biçeroğlu Ö. Novel insight into wound healing properties of methanol extract of Capparis ovata Desf. var. palaestina Zohary fruits. J Pharm Pharmacol 2018; 70: 1401–1413.
• 26. Amsterdam D. Susceptibility testing of antimicrobials in liquid media, In Antibiotics in Laboratory Medicine, Fourth Edition, V. Lorian (Ed.), Williams & Wilkins, Baltimore 1996: 52-111.
• 27. Clinical and Laboratuary Standarts Institute (CLSI) (Formerly, NCCLS) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, CLSI M7-A7, Clinical and Laboratory Standards Institute, 940 West Valley Road, Wayne, Pennsylvania, USA 2006.
• 28. Clinical and Laboratory Standards Institute. Susceptibility testing of Mycobacteria, Norcardiae, and Other Aerobic Actinomycetes; Approved Standard. CLSI document M24-A. Wayne, Pa. USA: CLSI; 2003.
• 29. Chung GA, Aktar Z, Jackson S, Duncan K, High-Throughput Screen for Detecting Antimycobacterial Agents. Antimicrob Agents Chemother 1995; 39(10); 2235–2238.
• 30. Lee SM, Kim J, Jeong J, Park YK, Bai G, Lee EY, Lee MK, Chang CL. Evaluation of the Broth Microdilution Method Using 2,3-Diphenyl-5- thienyl-(2)-tetrazolium Chloride for Rapidly Growing Mycobacteria Susceptibility Testing, J Korean Med Sci 2007; 22: 784-90.
• 31. McLafferty FW, Stauffer DB, The Wiley/NBS Registry of Mass Spectral Data, J Wiley and Sons, New York, 1989.32. Koenig WA, Joulain D, Hochmuth DH. Terpenoids and Related Constituents of Essential Oils, MassFinder 3, D. H. Hochmuth (ed.), Hamburg, Germany, 2004.