Modifiye altın nanoparçacıkların fare hipokampal kesitlerindeki nöronal fonksiyonlar üzerine etkileri

Yıl 2019, Cilt: 12 Sayı: 2, 328 - 340, 30.08.2019

Bilge Güvenç Tuna Yavuz Yavuz Gamze Kuku Akif Maharramov Bayram Yilmaz Melike Saricam Merve Ercan Mustafa Culha Soner Dogan

https://doi.org/10.26559/mersinsbd.594531

Cited By: 3

Öz

Amaç: Altın nanoparçacık (AuNP) modifikasyonlarının sinir sistemi
fonksiyonunu nasıl etkileyebileceği tam olarak bilinmemektedir. Bu çalışmada, AuNp’lerin
glikoz (GlikozAuNp), oligonükleotid (15 baz, d(A)15AuNP) veya peptid (negatif amino asit
içeren, AspAuNp) ile işlevselleştirilmiş formlarının fare hipokampal
nöronları üzerindeki biyoelektriksel etkisinin ex vivo olarak araştırılması amaçlanmıştır. Yöntem: AuNP modifikasyonları UV/Vis spektroskopi, Dinamik Işık
Saçılması (DLS) ve Geçirimli Elektron Mikroskobu (TEM) ile karakterize edildi
ve ex vivo olarak 4-6 haftalık Balb-c
erkek farelerin beyin hipokampüs bölgelerinden alınan kesitlere eklendi,
spontan ve uyarılmış aksiyon potansiyelleri (AP) yama kıskacı, tüm hücre
tekniği ile kaydedildi. Spontan AP’lerinin en büyük genlik değeri, yükselen
eğimi, yarı genişliği, iki pik arasında geçen süre ve ateşleme hızı analiz
edildi. Bulgular: Karakterizasyon
sonuçları AuNP’lerin yüzey modifikasyonlarının 5-7 nm çapında ve koloidal
olduğunu gösterdi. Spontan AP’lerin ateşleme hızı GlikozAuNp (n=9) gurubunda,
kontrol (n=9), AspAuNp (n=6) ve d(A)15AuNP (n=9) eklenen gruplara göre en fazla
olarak ölçüldü (sırasıyla 10.1±5.5 4.4±3.2 9.5±3.8 ve 6.2±3.0 pik/s, p>0.05). 

Ek olarak, uyarılmış AP’lerin özelliklerini
yansıtan akım voltaj (I-V) analizinde, GlikozAuNp (n=8) gurubunun akım
uyarılarına ((-150)–(+150 pA)) cevaben oluşturdukları membran potansiyelleri
kontrol (n=8) ve d(A)15AuNP (n=9) gruplarının cevabına göre negatif akım
bölgesinde negatife, pozitif akım uyarısı bölgesinde pozitife kaydı. Sonuç: Medikal uygulamalarda
kullanılacak AuNP’lerin yüzey modifikasyonlarının hipokampal nöronların
AP’lerinin ateşleme hızını arttırarak, patolojik koşulları tetikleyebilecekleri
ve daha biyouyumlu formların belirlenmesi gerektiği önerilebilir.

Anahtar Kelimeler

Altın nanoparçacıklar, aksiyon potansiyeli, Yüzey modifikasyonları

Destekleyen Kurum

TUBITAK

Proje Numarası

215S052

Teşekkür

Arastırmacılar TUBITAK'a destekleri icin tesekkur eder

The effect of modified gold nanoparticles on the function of neurons of mice hipocampal brain slices

Öz

Aim: It
is still unclear whether modifications of gold nanoparticles (AuNp) affect the
function of the nervous system. Aim of this study was to investigate the bioelectrical
effect of glucose (GlucoseAuNp), oligonükleotite (d(A)15AuNP) or peptide
(AspAuNp) modified AuNp, on hippocampal mouse neurons after ex vivo
administration. Methods: AuNP
modifications were characterized by UV/Vis spectroscopy, Dynamic Light
Scattering (DLS) and Transmission Electron Microscopy (TEM). Subsequently, AuNp
modifications were added ex vivo to sections of hippocampal regions obtained
from the brains of 4-6 week old male Balb-c mice, and then spontaneous and
stimulated action potentials (AP) were recorded by patch clamp with whole-cell
technique. The Peak amplitude, rising slope, half with and firing rate of Aps
were analyzed. Results:
Characterization results showed that AuNp modifications have 5-7 nm size and
colloidal shape. The firing rate of the APs measured in the GlucoseAuNp (n=9)
group was higher compared to the AspAuNp (n=7), d(A)15AuNP (n=9), and control
(n=9) groups (respectively 10.1±5.5 9.5±3.8, 6.2±3.0 pic/s, and 4.4±3.2, pic/s,
p>0.05). In addition, current voltage (I-V) analysis showed that the
membrane potential in response to current stimuli ((-150)–(150 pA)) of the
GlucoseAuNp (n=8) shifted negative for negative current stimulations and
positive for positive current stimulations compared to the control (n=8) and
d(A)15AuNP (n=9) groups. Conclusion:
The surface AuNp modifications will be used in medical applications might
increase the firing rate of Aps and stimulate neurological patholojies in
hippocampal neurons and it may be suggested that more biocompatible AuNp forms
should be identified. 

Anahtar Kelimeler

Gold nanoparticles, Action potential, Surface Modifications

Proje Numarası

215S052

Kaynakça

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