Year 2016, Volume , Issue , Pages 7 - 10 2016-05-26

THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2

Gultekin Akdamar [1] , Ozgur Ceylan [2] , Aysel Ugur [3]

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Streptomyces griseoflavus OC. 124-2 used in this study was isolated from the field soil of Dalaman Directorate of Agricultural Enterprises Muğla. As a result of phenotypic and molecular characterization, the isolate was identified as Streptomyces griseoflavus and named as OC. 124-2. The fermentation liquid of Streptomyces griseoflavus OC. 124-2 was obtained in optimum fermentation conditions, and then it was filtered and extracted with ethyl acetate 1:1. The extract containing the active compounds was obtained by evaporating the solvent. Biomonitor strains, Chromobacterium violaceum CV026 for the determination of anti-quorum sensing activity (anti-QS), Chromobacterium violaceum CV12472 for the determination of inhibition of violacein pigment production and Pseudomonas aeruginosa PA01 for the determination of anti-swarming activity were used at MIC and sub-MIC concentrations. The anti-quorum sensing and anti-swarming activities could not be detected for the extract. Violacein production was inhibited by 100%, 74.86%, 65.74% and 31.99% at MIC, MIC/2, MIC/4 and MIC/8 concentrations of the extract treatment, respectively. While the detected inhibition of violacein pigment production did not inhibit the bacterial growth, it was revealed that it inhibited the quorum-sensing-regulated signaling systems. Accordingly, it was shown that the active compounds obtained from ethyl acetate extract of OC. 124-2 constituted a non-selective pressure for the growth of drug resistant pathogen bacteria and they may be used as an alternative at treatment of these bacteria.
Subjects
Journal Section Journals
Authors

Author: Gultekin Akdamar

Author: Ozgur Ceylan

Author: Aysel Ugur

Bibtex @ { muglajsci269958, journal = {Mugla Journal of Science and Technology}, issn = {2149-3596}, address = {Muğla Sıtkı Koçman Üniversitesi}, year = {2016}, volume = {}, pages = {7 - 10}, doi = {}, title = {THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2}, key = {cite}, author = {Akdamar, Gultekin and Ceylan, Ozgur and Ugur, Aysel} }
APA Akdamar, G , Ceylan, O , Ugur, A . (2016). THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2. Mugla Journal of Science and Technology, (), 7-10. Retrieved from http://dergipark.org.tr/muglajsci/issue/25591/269958?publisher=mu
MLA Akdamar, G , Ceylan, O , Ugur, A . "THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2". Mugla Journal of Science and Technology (2016): 7-10 <http://dergipark.org.tr/muglajsci/issue/25591/269958?publisher=mu>
Chicago Akdamar, G , Ceylan, O , Ugur, A . "THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2". Mugla Journal of Science and Technology (2016): 7-10
RIS TY - JOUR T1 - THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2 AU - Gultekin Akdamar , Ozgur Ceylan , Aysel Ugur Y1 - 2016 PY - 2016 N1 - DO - T2 - Mugla Journal of Science and Technology JF - Journal JO - JOR SP - 7 EP - 10 VL - IS - SN - 2149-3596- M3 - UR - Y2 - 2019 ER -
EndNote %0 Mugla Journal of Science and Technology THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2 %A Gultekin Akdamar , Ozgur Ceylan , Aysel Ugur %T THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2 %D 2016 %J Mugla Journal of Science and Technology %P 2149-3596- %V %N %R %U
ISNAD Akdamar, Gultekin , Ceylan, Ozgur , Ugur, Aysel . "THE QUORUM SENSİNG INHIBITION ACTIVITY OF THE ETHYL ACETATE EXTRACT OF STREPTOMYCES GRİSEOFLAVUS OC. 124-2". Mugla Journal of Science and Technology / (May 2016): 7-10.