Isolation and identification of Streptomyces species isolated from forest area (Sakarya City) and determination the production of some extracellular enzyme

Yıl 2019, Cilt: 7 Sayı: 1, 64 - 71, 22.07.2019

Ömrüye Özok Kerem Özdemir

https://doi.org/10.33409/tbbbd.595174

Cited By: 2

Öz

In this study, forest area located in the
province of Sakarya city soil bacteria of the genus Streptomyces isolated and made computer-assisted diagnosis and
various enzyme activities of the isolates were determined. All isolates of
phenotypic and biochemical characteristics of the data being transmitted to the
computer program determined IDENTAX, diagnosis is made by determining their
position in the genus. Isolates in the color group analysis based on aerial and
substrate mycelium color is divided into four main groups. Diagnostic test
results of Streptomyces isolates are
completed by a computer program MVSP 3.2and were created similarity dendograms.
In addition, all isolates protease, amylase, lipase, cellulase and asparaginase
enzyme activity was determined.

Anahtar Kelimeler

Streptomyces, Extracellular enzyme, characterization

Sakarya ili ormanlık alan topraklarından Streptomyces türlerinin izolasyonu, karakterizasyonu ve bazı ekstraselüler enzimlerin üretiminin belirlenmesi

Öz

Bu çalışmada, Sakarya ili sınırlarında
bulunan ormanlık alan topraklarından Streptomyces
cinsi bakterileri izole edilerek bilgisayar yardımıyla teşhisleri yapılmış ve
izolatların amilaz, selülaz, L-asparaginaz, proteaz ve lipaz enzimlerinin
aktiviteleri belirlenmiştir. Yapılan renk grubu analizinde izolatlar havasal ve
misel rengi esas alınarak 4 ana gruba ayrılmıştır. Teşhisleri tamamlanan Streptomyces izolatlarının test
sonuçları MVSP 3.2 bilgisayar programı ile birbirleri arasındaki benzerlik
dendogramları oluşturulmuştur. Tüm izolatların fenotipik ve biyokimyasal
karakterleri belirlenerek IDENTAX bilgisayar programına bu veriler aktarılmış,
cins içerisindeki pozisyonları belirlenerek teşhisleri yapılmıştır. Bu
teşhislere göre 80 izolattan 49’unun Streptomyces
exfoliatus, 14’ünün Streptomyces
atroolivaceus, 1’i Streptomyces
albidoflavus,1’i Streptomyces
chromofuscus, 1’i de Streptomyces
purpureus olduğu belirlenmiştir. Ayrıca tüm izolatların proteaz, amilaz,
lipaz, selülaz ve asparaginaz enzim aktiviteleri belirlenmiştir.

Anahtar Kelimeler

Streptomyces, ekstraselüler enzim, karakterizasyon

Kaynakça

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