Year 2012, Volume 42, Issue 3, Pages 377 - 383 2012-03-01

Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study
Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study

Sema DÜNDAR [1] , Fatih ÖZCURA [2] , İbrahim METEOĞLU [3] , Mehmet Erkut KARA [4]

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To investigate the effects of smoking, reported as a risk factor for age related macular degeneration (ARMD), on the vascular endothelial growth factor (VEGF) expression and apoptosis in the retina and choroid of rats. Materials and methods: This experimental study included 38 Sprague-Dawley rats. The rats were randomly assigned into 4 groups. Groups 1 (males) and 2 (females) were exposed to smoke beginning on day 21 after birth, whereas groups 3 (males) and 4 (females) were not exposed to smoke. At the end of the fourth month, the right eyes of all of the rats were enucleated. Immunohistochemical analysis was performed in the enucleated eyes in terms of the VEGF and apoptosis markers, namely caspase-3, Bcl-X, and p53. Comparisons between the groups were performed using the Mann-Whitney U test. Results: The VEGF expression in the retina and choroid increased significantly in rats exposed to smoking. This significant difference did not change between the sexes. There were no significant differences in terms of Bcl-X and p53 expressions between the groups; however, the caspase-3 expression increased significantly in the photoreceptor layer in rats exposed to smoking. Conclusion: Our findings show that smoking significantly increases the VEGF expression in many of the retinal layers and choroid. Smoking also increases caspase-3 expression only in the photoreceptor layer. According to these findings, smoking may be a risk factor for retinal vascular disease such as exudative ARMD, via stimulating the VEGF.
To investigate the effects of smoking, reported as a risk factor for age related macular degeneration (ARMD), on the vascular endothelial growth factor (VEGF) expression and apoptosis in the retina and choroid of rats. Materials and methods: This experimental study included 38 Sprague-Dawley rats. The rats were randomly assigned into 4 groups. Groups 1 (males) and 2 (females) were exposed to smoke beginning on day 21 after birth, whereas groups 3 (males) and 4 (females) were not exposed to smoke. At the end of the fourth month, the right eyes of all of the rats were enucleated. Immunohistochemical analysis was performed in the enucleated eyes in terms of the VEGF and apoptosis markers, namely caspase-3, Bcl-X, and p53. Comparisons between the groups were performed using the Mann-Whitney U test. Results: The VEGF expression in the retina and choroid increased significantly in rats exposed to smoking. This significant difference did not change between the sexes. There were no significant differences in terms of Bcl-X and p53 expressions between the groups; however, the caspase-3 expression increased significantly in the photoreceptor layer in rats exposed to smoking. Conclusion: Our findings show that smoking significantly increases the VEGF expression in many of the retinal layers and choroid. Smoking also increases caspase-3 expression only in the photoreceptor layer. According to these findings, smoking may be a risk factor for retinal vascular disease such as exudative ARMD, via stimulating the VEGF.
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Primary Language tr
Journal Section Articles
Authors

Author: Sema DÜNDAR

Author: Fatih ÖZCURA

Author: İbrahim METEOĞLU

Author: Mehmet Erkut KARA

Dates

Publication Date: March 1, 2012

Bibtex @ { tbtkmedical147099, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {TUBITAK}, year = {2012}, volume = {42}, pages = {377 - 383}, doi = {}, title = {Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study}, key = {cite}, author = {DÜNDAR, Sema and ÖZCURA, Fatih and METEOĞLU, İbrahim and KARA, Mehmet Erkut} }
APA DÜNDAR, S , ÖZCURA, F , METEOĞLU, İ , KARA, M . (2012). Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study. Turkish Journal of Medical Sciences, 42 (3), 377-383. Retrieved from http://dergipark.org.tr/tbtkmedical/issue/12298/147099
MLA DÜNDAR, S , ÖZCURA, F , METEOĞLU, İ , KARA, M . "Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study". Turkish Journal of Medical Sciences 42 (2012): 377-383 <http://dergipark.org.tr/tbtkmedical/issue/12298/147099>
Chicago DÜNDAR, S , ÖZCURA, F , METEOĞLU, İ , KARA, M . "Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study". Turkish Journal of Medical Sciences 42 (2012): 377-383
RIS TY - JOUR T1 - Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study AU - Sema DÜNDAR , Fatih ÖZCURA , İbrahim METEOĞLU , Mehmet Erkut KARA Y1 - 2012 PY - 2012 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 377 EP - 383 VL - 42 IS - 3 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2019 ER -
EndNote %0 Turkish Journal of Medical Sciences Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study %A Sema DÜNDAR , Fatih ÖZCURA , İbrahim METEOĞLU , Mehmet Erkut KARA %T Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study %D 2012 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 42 %N 3 %R %U
ISNAD DÜNDAR, Sema , ÖZCURA, Fatih , METEOĞLU, İbrahim , KARA, Mehmet Erkut . "Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study". Turkish Journal of Medical Sciences 42 / 3 (March 2012): 377-383.
AMA DÜNDAR S , ÖZCURA F , METEOĞLU İ , KARA M . Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study. Turkish Journal of Medical Sciences. 2012; 42(3): 377-383.
Vancouver DÜNDAR S , ÖZCURA F , METEOĞLU İ , KARA M . Effects of long-term passive smoking on the vascular endothelial growth factor and apoptosis marker expression in the retina and choroid: an experimental study. Turkish Journal of Medical Sciences. 2012; 42(3): 383-377.