Year 2015, Volume 45, Issue 6, Pages 1285 - 1291 2015-12-04

miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa
miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa

TANG WENHAO [1] , LIU DE-FENG [2] , HONG KAI [3] , ZHAO LIAN-MING [4] , MAO JIA-MING [5] , ZHUANG XIN-JIE [6] , MA LU-LIN [7] , JIANG HUI [8]

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Background/aim: Heat shock proteins (HSPs) are expressed in human spermatozoa and play a role in sperm function. MicroRNAs (miRNAs) regulate gene expression, and the possible involvement of miRNAs in the regulation of HSP gene expression in sperm was investigated in this study. Materials and methods: miRNAs differentially expressed in 8 copies of an oligoasthenozoospermic semen group (OA) were identified by comparison with a normal male semen control group (NC) using microarray technology. Potential targets of HSP proteins among the differentially expressed miRNAs were further investigated. Results: HSP40, HSP60, HSP70, and HSP90 were all found to be expressed in human ejaculated spermatozoa. A total of 32 miRNAs showed significant differences in expression between the OA and NC groups. Ten of these miRNAs encoded potential targets of HSPs. Conclusion: These results show that specific miRNAs are expressed in human ejaculated spermatozoa. These miRNAs appear to be involved in regulating the expression of HSP40, HSP70, and HSP90, and this in turn affects sperm function.
Background/aim: Heat shock proteins (HSPs) are expressed in human spermatozoa and play a role in sperm function. MicroRNAs (miRNAs) regulate gene expression, and the possible involvement of miRNAs in the regulation of HSP gene expression in sperm was investigated in this study. Materials and methods: miRNAs differentially expressed in 8 copies of an oligoasthenozoospermic semen group (OA) were identified by comparison with a normal male semen control group (NC) using microarray technology. Potential targets of HSP proteins among the differentially expressed miRNAs were further investigated. Results: HSP40, HSP60, HSP70, and HSP90 were all found to be expressed in human ejaculated spermatozoa. A total of 32 miRNAs showed significant differences in expression between the OA and NC groups. Ten of these miRNAs encoded potential targets of HSPs. Conclusion: These results show that specific miRNAs are expressed in human ejaculated spermatozoa. These miRNAs appear to be involved in regulating the expression of HSP40, HSP70, and HSP90, and this in turn affects sperm function.
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Primary Language tr
Subjects
Journal Section Articles
Authors

Author: TANG WENHAO

Author: LIU DE-FENG

Author: HONG KAI

Author: ZHAO LIAN-MING

Author: MAO JIA-MING

Author: ZHUANG XIN-JIE

Author: MA LU-LIN

Author: JIANG HUI

Bibtex @ { tbtkmedical149013, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {TUBITAK}, year = {2015}, volume = {45}, pages = {1285 - 1291}, doi = {}, title = {miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa}, key = {cite}, author = {WENHAO, TANG and DE-FENG, LIU and KAI, HONG and LIAN-MING, ZHAO and JIA-MING, MAO and XIN-JIE, ZHUANG and LU-LIN, MA and HUI, JIANG} }
APA WENHAO, T , DE-FENG, L , KAI, H , LIAN-MING, Z , JIA-MING, M , XIN-JIE, Z , LU-LIN, M , HUI, J . (2015). miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa. Turkish Journal of Medical Sciences, 45 (6), 1285-1291. Retrieved from http://dergipark.org.tr/tbtkmedical/issue/12391/149013
MLA WENHAO, T , DE-FENG, L , KAI, H , LIAN-MING, Z , JIA-MING, M , XIN-JIE, Z , LU-LIN, M , HUI, J . "miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa". Turkish Journal of Medical Sciences 45 (2015): 1285-1291 <http://dergipark.org.tr/tbtkmedical/issue/12391/149013>
Chicago WENHAO, T , DE-FENG, L , KAI, H , LIAN-MING, Z , JIA-MING, M , XIN-JIE, Z , LU-LIN, M , HUI, J . "miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa". Turkish Journal of Medical Sciences 45 (2015): 1285-1291
RIS TY - JOUR T1 - miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa AU - TANG WENHAO , LIU DE-FENG , HONG KAI , ZHAO LIAN-MING , MAO JIA-MING , ZHUANG XIN-JIE , MA LU-LIN , JIANG HUI Y1 - 2015 PY - 2015 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 1285 EP - 1291 VL - 45 IS - 6 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2019 ER -
EndNote %0 Turkish Journal of Medical Sciences miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa %A TANG WENHAO , LIU DE-FENG , HONG KAI , ZHAO LIAN-MING , MAO JIA-MING , ZHUANG XIN-JIE , MA LU-LIN , JIANG HUI %T miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa %D 2015 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 45 %N 6 %R %U
ISNAD WENHAO, TANG , DE-FENG, LIU , KAI, HONG , LIAN-MING, ZHAO , JIA-MING, MAO , XIN-JIE, ZHUANG , LU-LIN, MA , HUI, JIANG . "miRNA-mediated regulation of heat shock proteins in human ejaculated spermatozoa". Turkish Journal of Medical Sciences 45 / 6 (December 2015): 1285-1291.