BIOLOGICAL CONTROL OF AFLATOXIGENIC FUNGI ON PEANUT: FOR THE PRE-HARVEST APPROACH

Yıl 2019, Cilt: 24 Sayı: 1, 21 - 27, 14.06.2019

İsılay Lavkor Halis Arıoglu İsıl Var Sevcan Oztemız

https://doi.org/10.17557/tjfc.562634

Cited By: 3

Öz

This study was carried out to determine the efficacy of different applications of a biopesticide for reduction of aflatoxin contamination in peanut. The biopesticide, afla-guard, delivers a nontoxigenic Aspergillus flavus to the field where it competes with naturally occurring toxigenic fungus. Biocontrol treatments included: (ı) soil application during sowing, (ıı) multiple application during sowing and 40 days after planting, (ııı) foliar application at 60 days after planting (ıv) control (untreated plots). Biopesiticide was applied to peanut plots in 2015 and 2016 in Randomized Complete Block Design with four replications. Peanuts were collected from control and treated plots at harvest-drying-pre-storage periods and analysed for aflatoxins. Aflatoxin concentrations were generally quite low in 2015, also the aflatoxin concentration in treated samples (from 0.04 to 0.71 μg/kg) was reduced by 97.38 to 99.82% compared with controls (from 21.84 to 27.12 μg/kg). In 2016, reductions were also noted for all biocontrol treatments (from 89.07 to 92.39%) compared with controls. In conjunction with the reductions in aflatoxin contamination, biocontrol treatments produced significant reductions with biopesticide in peanut. Therefore, it can be said that a biological control method is a promising approach for controlling aflatoxin.

Anahtar Kelimeler

Aspergillus flavus, Aspergillus flavus NRRL 21882, Biological control, Peanuts

Destekleyen Kurum

The Scientific Technological Research Council of Turkey (TUBITAK).

Proje Numarası

115O007

Teşekkür

This study (Project No 115O007) was financial supported by The Scientific Technological Research Council of Turkey (TUBITAK).

Kaynakça

• Anonymous, 2014. http://www.syngenta-us.com/currentlabel/ afla-guard-gr. Afla-Guard® GR
• Anonymous, 2017. The meteorological data for Adana. The Turkish State Meteorological Service Adana Regional Directorship, 2015, 2016.
• Arıoglu, H. 2000. Peanuts cultivation breeding. Adana, Turkey: Cukurova University Faculty of Agriculture publishing.
• Arzandeh, S. and S. Jinap. 2011. Effect of initial aflatoxin concentration, heating time and roasting temperature on aflatoxin reduction in contaminated peanuts and process optimization using response surface modeling. Int. J. Food Sci. Technol. 46: 485–491.
• Atehnkeng, J., P.S. Ojiambob, P.J. Cotty and R. Bandyopadhyay. 2014. Field efficacy of a mixture of atoxigenic Aspergillus flavus Link: FR vegetative compatibility groups in preventing aflatoxin contamination in maize (Zea mays L.). Biol. Control, 72: 62-70.
• Chepsergon, J., E.K. Kiprop, L.A. Mwamburi. Biocontrol of aflatoxins in corn using atoxigenic Aspergillus flavus. Review International Journal of Science and Research. 12:1954-1958.
• Cole, R.J., T.H. Sanders, J.W. Dorner and P.D. Blankenship. 1989. Environmental conditions required to induce preharvest aflatoxin contamination of groundnuts: Summary of six years’ research. In: Aflatoxin contamination of groundnut: Proceedings of the international workshop, ed. Hall SD., 279-287, ICRISAT Center, Patancheru, AP, India.
• Denizel, T. and O. Kosker. 1972. A mycological survey of various kinds of nuts commercially available in the UK with reference to mycotoxins. University of Ankara, Turkey: Yearbook of the Faculty of Agriculture.
• Dorner, J.W., R.J. Cole and P.D. Blankenship. 1992. Use of a biocompetitive agent to control preharvest aflatoxin in drought stressed peanuts. J. Food Prot. 55: 888-892.
• Dorner, J.W., R.J. Cole and P.D. Blankenship. 1998. Effect of inoculum rate of biological control agents on preharvest aflatoxin contamination of peanuts. Biol. Cont. 12: 171-176.
• Dorner, J.W. and R.J. Cole. 2002. Effect of application of nontoxigenic strains of Aspergillus flavus and A. parasiticus on subsequent aflatoxin contamination of peanuts in storage. J. Stored Prod. Res. 38: 329-339.
• Dorner, J.W., R.J. Cole, W.J. Connick, D.J. Daigle, M.R. Mcguire and B.S. Shashac. 2003. Evaluation of biological control formulations to reduce aflatoxin contamination in peanuts. Biol. Cont. 26: 318-324.
• Dorner, J.W. 2004. Combined effects of biological control formulations, cultivars, and fungicides on preharvest colonization and aflatoxin contamination of peanuts by Aspergillus species. Peanut Sci. 31: 79–86.
• Dorner, J.W. 2005. Biological control of aflatoxin crop contamination. In: Aflatoxin and food safety, ed. Abbas H.K., 333–352, Taylor and Francis, New York, USA.
• Dorner, J.W. and M.C. Lamb. 2006. Development and commercial use of Afla-Guard(®) an aflatoxin biocontrol agent. Mycotoxin Res. 22: 33-38.
• Dorner, J.W. and B.W. Horn. 2007. Separate and combined applications of nontoxigenic Aspergillus flavus and A. parasiticus for biocontrol of aflatoxin in peanuts. Mycopathologia 163:215–223.
• Dorner, J.W. 2008. Management and prevention of mycotoxins in peanuts. Food Additives and Contaminants. 25: 203–208.
• Dorner, J.W. 2009. Development of biocontrol technology to manage aflatoxin contamination in peanuts. Peanut Sci. 36: 60-67.
• Dorner, J.W. 2010. Efficacy of a biopesticide for control of aflatoxins in corn. J. Food Prot. 73: 495–499.
• Gomez, A.K. and A.A. Gomez. 1983. Statistical Procedures for Agricultural Research. New York: USA: Wiley.
• IARC. 1993. Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. International Agency for Research on Cancer. http://monographs.iarc.fr/ENG/Monographs/vol56/mono56.p df, (Accessed 21 June 2017)
• Isakeit, T., S. Murray and K. Mayfield. 2010. Efficacy of Afla- Guard (Aspergillus flavus NRRL 21882) to control aflatoxin on corn in Burleson County, Texas, 2009. Plant Disease Management Reports 4: FC081. www. plantmanagementnetwork.org/pub/trial/PDMR/reports /2010/FC081.pdf, (Accessed January 20, 2018)
• Kumar, V., M.S. Basu and T.P. Rajendran. 2008. Mycotoxin research and mycoflora in some commercially important agricultural commodities. Crop Prot. 27: 891–905.
• Lavkor, I. 2013. Control of diseases and aflatoxin occurrences with proper cultural and disease management practices in peanut growing. Ph.D. Cukurova University, Adana, p. 300.
• Lavkor, I. and M. Bicici. 2015. Aflatoxin occurrence in peanuts grown in Osmaniye at harvest, post-harvest, drying and prestorage periods. J. Agric. Sci. 21: 394-405.
• Lavkor, I., I. Var, S. Oztemiz and M. Almatar. 2017. First report of mycotoxins in second peanuts crop in Adana and Osmaniye at harvest, drying, pre-storage and storage periods. In: Aflatoxin-Control, Analysis, Detection and Health Risks, ed. Abdulra’uf L B, 193-208. InTech Open Science, Rijeka, Croatia.
• Makun, H.A., S.T. Anjorin, B. Moronfoye, F. Adejo, O.A. Afolabi, G. Fagbayibo, B.O. Balogun, and A.A. Surajudeen. 2010. Fungal and aflatoxin contamination of some human food commodities in Nigeria. Afr. J. Food Sci. 4: 127-135.
• Mutegi, C.K., H.K. Ngugi, S.L. Hendiks and R.B. Jones. 2012. Factors associated with the incidence of Aspergillus section Flavi and aflatoxins contamination of peanuts in Busia and Homa bay districts of Western Kenya. Plant Pathol. J. 61: 1143-1153.
• Pitt, J.I. and Hocking, A.D. 2006. Mycotoxins in Australia: biocontrol of aflatoxin in peanuts. Mycopathologia, 162: 233-243
• Richard, J.L. 2007. Some major mycotoxins and their mycotoxicoses- an overview. Int. J. Food Microbiol. 119: 3- 10.
• Russell, R., M. Paterson and N. Lima. 2010. How the climate change affect mycotoxins in food? Food Res. Int. 43: 1902- 1914.
• Sigh, P., J. March and P.J. Cotty. Aspergillus texensis: a novel aflatoxin producer with S morphology from the United States. Toxins 2018, 10, 513.
• Waliyar, F., S.V. Reddy and P.L. Kumar. 2009. Review of immunological methods for the quantification of aflatoxins in peanut and other foods. Peanut Sci. 36: 54-59.
• Wu, F. and P. Khlangwiset. 2010. Health economic impacts and cost-effectiveness of aflatoxin reduction strategies in Africa: Case studies in biocontrol and postharvest interventions. Food Addit. Contam. A. 27: 496-509.
• Yu, J., P.K. Chang, K.C. Ehrlich, J.W. Cary, D. Bhatnagar, T.E. Cleveland, G.A. Payne, J.E. Linz, J.E., C.P. Woloshuk and J.W. Bennett. 2004. Clustered pathway genes in aflatoxin biosynthesis. Appl. Environ. Microbiol. 70: 1253-1262.
• Yin, Y., L. Yan, J. Jiang and Z. Ma.2008. Biological control of aflatoxin contamination of crops. Journal of Zhejiang University, Science B.9:787-792.