Year 2019, Volume 4, Issue 1, Pages 15 - 21 2019-05-16

Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme

Aykut Öztekin [1]

21 51

Peroxidases (PODs) are a group of enzymes that are commonly found in bacteria, fungi, plants and animals and have important uses in the food, pharmaceutical industry and clinical diagnostics. Lactoperoxidase (LPO) enzyme, which is included in peroxidase enzyme class, is found milk, saliva and tears in mammals. With thiocyanate and hydrogen peroxide, it forms one of the body's defense systems against infections. Flavonoid derivatives are abundant in plants and constitute an important part of the diet. These derivatives, which are produced synthetically or naturally, have many pharmacological activities. In this study, the effect of some flavonoid derivatives (5,7-Dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-4H-chromen-4-one (a), 3,5,7-Trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one (b), 7-Hydroxy-4'-nitroisoflavone (c), 6-Fluoroflavone (d), 7-Hydroxy-3-(4-methoxyphenyl)-4H-chromen-4-one (e), 7-Methoxy-2-phenyl-4H-chromen-4-one (f)) on the lactoperoxidase enzyme was investigated. Firstly, by using Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography, the LPO enzyme was purified 65 fold (with a yield of 23%) from bovine milk and kinetic studies were carried out with flavonoid derivatives using this enzyme. The Ki values of six molecules were found in ranging from 7.85 µM to 0.023 μM. 6-Fluoroflavone was the most effective inhibitor with Ki value of 0.023 μM.

Lactoperoxidase, flavonoid derivatives, inhibition
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Primary Language en
Subjects Science
Journal Section Volume IV, Issue I, 2019
Authors

Orcid: 0000-0003-1418-179X
Author: Aykut Öztekin (Primary Author)
Institution: AGRI IBRAHIM CECEN UNIVERSITY
Country: Turkey


Dates

Publication Date: May 16, 2019

Bibtex @research article { tjos537649, journal = {Turkish Journal of Science}, issn = {2587-0971}, eissn = {2587-0971}, address = {KED Yayıncılık LTD ŞTİ.}, year = {2019}, volume = {4}, pages = {15 - 21}, doi = {}, title = {Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme}, key = {cite}, author = {Öztekin, Aykut} }
APA Öztekin, A . (2019). Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme. Turkish Journal of Science, 4 (1), 15-21. Retrieved from http://dergipark.org.tr/tjos/issue/45237/537649
MLA Öztekin, A . "Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme". Turkish Journal of Science 4 (2019): 15-21 <http://dergipark.org.tr/tjos/issue/45237/537649>
Chicago Öztekin, A . "Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme". Turkish Journal of Science 4 (2019): 15-21
RIS TY - JOUR T1 - Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme AU - Aykut Öztekin Y1 - 2019 PY - 2019 N1 - DO - T2 - Turkish Journal of Science JF - Journal JO - JOR SP - 15 EP - 21 VL - 4 IS - 1 SN - 2587-0971-2587-0971 M3 - UR - Y2 - 2019 ER -
EndNote %0 Turkish Journal of Science Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme %A Aykut Öztekin %T Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme %D 2019 %J Turkish Journal of Science %P 2587-0971-2587-0971 %V 4 %N 1 %R %U
ISNAD Öztekin, Aykut . "Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme". Turkish Journal of Science 4 / 1 (May 2019): 15-21.
AMA Öztekin A . Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme. TJOS. 2019; 4(1): 15-21.
Vancouver Öztekin A . Determination of Some Flavonoid Derivatives Inhibitory Effect on Bovine Milk Lactoperoxidase Enzyme. Turkish Journal of Science. 2019; 4(1): 21-15.