TY  - JOUR
T1  - Derin Öğrenme (CNN, RNN, LSTM, GRU) Kullanarak Protein İkincil Yapı Tahmini
TT  - Using Deep Learning (CNN, RNN, LSTM, GRU) methods for the prediction of Protein Secondary Structure
AU  - Çakmak, Ezgi
AU  - Selvi, İhsan Hakan
PY  - 2022
DA  - June
DO  - 10.26650/acin.1008075
JF  - Acta Infologica
JO  - ACIN
PB  - İstanbul Üniversitesi
WT  - DergiPark
SN  - 2602-3563
SP  - 43
EP  - 52
VL  - 6
IS  - 1
LA  - tr
AB  - Protein, canlı organizmaların biyolojik süreçlerinde çok önemli bir role sahiptir. Proteinin işlevini bilmek, biyoloji ve tıp alanında gelecekteki çalışmalara büyük katkı sağlar. Proteinin fonksiyonunu anlamak için üç boyutlu yapısını anlamak önemlidir. Protein yapısını çözümlemek için X-ışını kristalografisi ve NMR gibi deneysel yöntemler kullanılmasına rağmen, sonuçların yetersiz olduğu kanıtlanmıştır. Bu nedenle, proteinlerin üç boyutlu yapısının tahmini, süreçlerdeki en önemli konulardan biri haline gelmektedir. Birincil yapı olarak bilinen amino asit dizisinden proteinin üç boyutlu şeklinin belirlenmesi zorlu olarak tanımlandığından, ikincil yapının tahmin edilmesi bu konuda önemli bir rol oynamaktadır. Literatürde protein ikincil yapısını tahmin etmek için makine öğrenmesi ve son zamanlarda derin öğrenme gibi birçok yöntem kullanılmıştır. Bu makale, yaygın olarak uygulanan dört derin öğrenme yöntemi olan CNN, RNN, LSTM ve GRU kullanılarak geliştirilen modellerin performanslarının bir karşılaştırmasını sağlamayı amaçlamaktadır. Bu modellerin eğitimi ve test edilmesi amacıyla CB513 veri seti kullanılmış, buna ek olarak doğruluk, f1 skoru, doğruluk ve kesinlik gibi performans değerlendirme ölçütleri uygulanmıştır. CNN, RNN, LSTM ve GRU modellerinin doğruluk oranları sırasıyla %82,54, %82,06, %81,1 ve %81,48’dir.
KW  - Protein İkincil Yapı Tahmini
KW  - CNN
KW  - RNN
KW  - GRU
N2  - Proteins play a crucial function in the biological processes of living organisms. Knowing the function of the protein offers significant insight into future biological and medical research. Since a protein’s shape determines its function, it is important to understand the protein’s 3D structure. Although experimental methods such as X-ray crystallography and nuclear magnetic resonance (NMR) have been used to examine the shape of proteins, so far the results have been insufficient. As a result, predicting the 3D structure of proteins is crucial. Determining the 3D structure of a protein from its primary structure is challenging. Therefore, predicting the protein secondary structure becomes important for studying its structure and function. Many emerging methods, including machine learning, as well as deep learning, have been used to predict the secondary structure of proteins and comprise a crucial part of Structural Bioinformatics. The goal of this study is to compare the results generated by predictive models that were created using the four most frequently utilized deep learning methods: convolutional neural networks (CNN), recurrent neural networks (RNN), long short term memory networks (LSTM), and gated recurrent units (GRU). The CB513 dataset was used to train and test these models, and performance evaluation metrics viz. accuracy, f1 score, recall, and precision were applied. The CNN, RNN, LSTM, and GRU models had an accuracy of 82.54%, 82.06%, 81.1%, and 81.48%, respectively.
CR  - Allison, L. A. (2007). From gene to protein. In Fundamental Molecular Biology (1. Baskı). Blackwell Publishing. google scholar
CR  - Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z, Miller, W., & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25(17), 3389-3402. https://doi.org/10.1093/nar/25.17.3389 google scholar
CR  - Aydin, Z., Kaynar, O., & Görmez, Y. (2018). Comparison of NR and UniClust databases for protein secondary structure prediction. 2018 26th Signal Processing and Communications Applications Conference (SIU), 1-4. https://doi.org/10.1109/SIU.2018.8404285 google scholar
CR  - Baldi, P., Brunak, S., Frasconi, P., Soda, G., & Pollastri, G. (1999). Exploiting the past and the future in protein secondary structure prediction. Bioinformatics, 15(11). https://doi.org/10.1093/bioinformatics/15.11.937 google scholar
CR  - Branden, C. I., & Tooze, J. (2012). Introduction to Protein Structure. In Introduction to Protein Structure. https://doi.org/10.1201/9781136969898 google scholar
CR  - Chen, C., Tian, Y., Zou, X., Cai, P., & Mo, J. (2007). Prediction of protein secondary structure content using support vector machine. Talanta. https://doi. org/10.1016/j.talanta.2006.09.015 google scholar
CR  - Chou, P. Y., & Fasman, G. D. (1974). Prediction of Protein Conformation. Biochemistry. https://doi.org/10.1021/bi00699a002 google scholar
CR  - Colab. (n.d.). Retrieved from https://research.google.com/colaboratory/intl/tr/faq.html google scholar
CR  - Cuff, J. A., & Barton, G. J. (1999). Evaluation and improvement of multiple sequence methods for protein secondary structure prediction. Proteins: Structure, Function, and Bioinformatics, 34(4), 508-519. https://doi.org/https://doi.org/10.1002/(SICI)1097-0134(19990301)34:4 <508::AID-PROT10>3.0.CO;2-4 google scholar
CR  - Dill, K. A., & MacCallum, J. L. (2012). The protein-folding problem, 50 years on. Science. https://doi.org/10.1126/science.1219021 google scholar
CR  - Garnier, J., Osguthorpe, D. J., & Robson, B. (1978). Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. Journal of Molecular Biology. https://doi.org/10.1016/0022-2836(78)90297-8 google scholar
CR  - Heffernan, R., Yang, Y., Paliwal, K., & Zhou, Y. (2017). Capturing non-local interactions by long short-term memory bidirectional recurrent neural networks for improving prediction of protein secondary structure, backbone angles, contact numbers and solvent accessibility. Bioinformatics. https:// doi.org/10.1093/bioinformatics/btx218 google scholar
CR  - Kneller, D. G., Cohen, F. E., & Langridge, R. (1990). Improvements in protein secondary structure prediction by an enhanced neural network. Journal of Molecular Biology. https://doi.org/10.1016/0022-2836(90)90154-E google scholar
CR  - Liu, H. L., & Hsu, J. P. (2005). Recent developments in structural proteomics for protein structure determination. Proteomics. https://doi.org/10.1002/ pmic.200401104 google scholar
CR  - Mirabello, C., & Pollastri, G. (2013). Porter, PaleAle 4.0: High-accuracy prediction of protein secondary structure and relative solvent accessibility. Bioinformatics. https://doi.org/10.1093/bioinformatics/btt344 google scholar
CR  - Moraes, I., Evans, G., Sanchez-Weatherby, J., Newstead, S., & Stewart, P. D. S. (2014). Membrane protein structure determination—the next generation. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1838(1), 78-87. google scholar
CR  - Nguyen, M. N., & Rajapakse, J. C. (2003). Multi-class support vector machines for protein secondary structure prediction. Genome Informatics. International Conference on Genome Informatics. https://doi.org/10.11234/gi1990.14.218 google scholar
CR  - Pollastri, G., & McLysaght, A. (2005). Porter: A new, accurate server for protein secondary structure prediction. Bioinformatics. https://doi.org/10.1093/ bioinformatics/bti203 google scholar
CR  - Rost, B., & Sander, C. (2000). Third generation prediction of secondary structures. In Webster, D. (Ed.), Protein Structure Prediction: Methods and Protocols (pp. 71-95). https://doi.org/10.1385/1-59259-368-2:71 google scholar
CR  - Rost, B. (2003). Rising Accuracy of Protein Secondary Structure Prediction. 207-249. google scholar
CR  - Rost, Burkhard, & Sander, C. (1994). Combining evolutionary information and neural networks to predict protein secondary structure. Proteins: Structure, Function, and Bioinformatics. https://doi.org/10.1002/prot.340190108 google scholar
CR  - Salamov, A. A., & Solovyev, V. V. (1995). Prediction of protein secondary structure by combining nearest-neighbor algorithms and multiple sequence alignments. Journal of Molecular Biology. https://doi.org/10.1006/jmbi.1994.0116 google scholar
CR  - Wang, J., Cheng, J., Zhao, Z., & Lu, W. (2019). Protein Secondary Structure Prediction Using Ensemble of LSTM Neural Networks. 2019 2nd International Conference on Information Systems and Computer Aided Education, ICISCAE 2019. https://doi.org/10.1109/ICISCAE48440.2019.221626 google scholar
CR  - Wang, Y., Mao, H., & Yi, Z. (2017). Protein secondary structure prediction by using deep learning method. Knowledge-Based Systems, 118, 115-123. https://doi.org/10.1016/j.knosys.2016.11.015 google scholar
CR  - Yi, T. M., & Lander, E. S. (1993). Protein secondary structure prediction using nearest-neighbor methods. Journal of Molecular Biology. https://doi.org/10.1006/jmbi.1993.1464 google scholar
UR  - https://doi.org/10.26650/acin.1008075
L1  - https://dergipark.org.tr/tr/download/article-file/2020485
ER  -