TY  - JOUR
T1  - Düzeltme: Adıyaman'da Yetişen Endemik Serratula olygocephala’nın Moleküler Karakterizasyonu ve Bazı Biyokimyasal Özelliklerini Belirlenmesi
TT  - Molecular Characterization and Determination of Some Biochemical Properties of Endemic Serratula olygocephala Growing in Adıyaman
AU  - Tekeli, Yener
AU  - Özkaya, Ahmet
AU  - Tel, Ahmet Zafer
AU  - Deniz Sönmez, Görkem
AU  - Akocak, Süleyman
PY  - 2021
DA  - December
Y2  - 2021
DO  - 10.31594/commagene.1033243
JF  - Commagene Journal of Biology
JO  - Comm. J. Biol.
PB  - ABADER (Adıyaman Bilimsel Araştırmalar Derneği)
WT  - DergiPark
SN  - 2602-456X
SP  - 222
EP  - 222
VL  - 5
IS  - 2
LA  - tr
AB  - Bu çalışmada Adıyaman'da Yetişen Endemik Serratula olygocephala’nın moleküler karakterizasyonu yapıldı. DNA izolasyonu Plant DNeasy (Qiagen Inc.) kiti kullanılarak, kit protokolü takip edilerek yapıldı. Bunun yanında bitkinin antioksidan aktivitesi ve yağ asidi kompozisyonu da belirlenmiştir. Antioksidan etkinliği bakımından, bitkinin toplam fenolik madde konsantrasyonu folin yöntemiyle belirlendi ve serbest radikal süpürme kapasitesini ise invitro olarak DPPH radikal süpürme testi ile tayin edildi. Serratula olygocephala’nın yağ asidi kompozisyonu ise gaz kromatografi yöntemiyle tayin edildi. Doymuş, tekli doymamış ve çoklu doymamış yağ asitleri yüzdesi belirlenmiştir.
KW  - Serratula oligocephala
KW  - 
KW  - moleküler karakterizasyon
KW  - antioksidan
KW  - serbest radikal
KW  - yağ asidi
N2  - In this study, molecular characterization of endemic Serratula olygocephala grown in Adiyaman was determined. DNA isolation was performed using the Plant DNeasy (Qiagen Inc.) kit, following the kit protocol. In addition, the antioxidant activity and fatty acid composition of the plant were also determined. In terms of the antioxidant activity, total phenolic substance concentration of the plant was determined by applying the folin method and free radical scavenging capacity was determined by DPPH radical scavenging test. Fatty acid profile of Serratula oligocephala was determined by gas chromatography. The percentage of the saturated, monounsaturated, and polyunsaturated fatty acids was calculated.
CR  - Sang, T., Crawford, D.J., & Stuessy, T.F. (1995). Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: Implications for biogeography and concerted evolution. Proceedings of the National Academy of Sciences of the United States of America, 92, 6813-6817. https://doi.org/10.1073/pnas.92.15.6813
CR  - Taberlet, P., Gielly, L., Pautou, G., & Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17, 1105–1109.
CR  - Tekeli, Y., Özkaya, A., Tel, A.Z., Deniz Sönmez, G., & Akocak, S. (2019). Molecular Characterization and Determination of Some Biochemical Properties of Endemic Serratula olygocephala Growing in Adıyaman. Commagene Journal of Biology, 3 (1), 32-36. https://doi.org/10.31594/commagene.789682
CR  - White T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, In: M Innis, D Gelfald, J Sninsky, T White, (eds.) PCR Protocols: A Guide to Methods and Applications. pp. 315-322. San Francisco: Academic Press. https://doi.org/10.1590/0102-33062015abb0267
UR  - https://doi.org/10.31594/commagene.1033243
L1  - https://dergipark.org.tr/tr/download/article-file/2118245
ER  -