@article{article_1069467, title={Investigation of Some Biological Properties of Coelomic Fluid Originating from the Red Worm Eisenia fetida}, journal={Karaelmas Fen ve Mühendislik Dergisi}, volume={12}, pages={183–193}, year={2022}, DOI={10.7212/karaelmasfen.1069467}, author={Demirkan, Elif and Sevgi, Tuba and Aybey, Aynur and Kocakurt, Halime}, keywords={Eisenia fetida, Antimikrobiyal aktivite, Sölomik sıvı, Solucan, Eisenia fetida, Enzim aktivitesi, Protein profili, Eisenia fetida, Enzim aktivitesi, Protein profili}, abstract={Eisenia fetida is abundant and vital member of the soil environment. The coelomic fluid they secrete help their physiological activities. This liquid, which they release during stress, also has a significant effect on soil health and ecotoxicity. In this study, some biological properties of coelomic fluid were investigated. For this purpose, 3 bacteria were selected from coelomic fluid, they were identified based on the 16S rRNA sequence and 2 of these were similar to Bacillus cereus and 1 to Bacillus thuringiensis. When the enzymatic activities in the coelomic fluid were controlled, the presence of lipase, cellulase, amylase, protease, phytase and arylsulfatase activities were detected. The antibacterial effects of the coelomic fluid was investigated against Enterococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhimurium, Yersinia enterocolitica and Pseudomonas aeruginosa. According to the results, it showed inhibitory effects on test bacteria. Additionally, the thin layer chromatography results showed the presence of glucose and maltose in the coelomic fluid. Protein pattern of fluid showed five clear protein bands in the range of 17–46 kDa. With this study, some biological properties of coelomic fluid have been revealed, and will shed light on future studies.}, number={2}, publisher={Zonguldak Bülent Ecevit Üniversitesi}