@article{article_1090986, title={Determination of Biocalcification Properties of Bacillus Species Isolated from Soil}, journal={Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi}, volume={38}, pages={147–154}, year={2022}, author={Öztürk Küp, Fatma and Eroğlu, Fatma}, keywords={Biyokalsifikasyon, Bacillus, 16S rRNA, Ureaz aktivitesi}, abstract={This study aimed to determine isolation of Bacillus species having urease activityfrom soil, morphological and molecular identification and on biocalcification features. Total 11 bacteria belong to Bacillus species were isolated from soilsamples. Identification of the isolates was carried out with 16S rRNA sequencing analysis. Urease activity of the isolates was carried out according to the phenol-hypochlorite method. CaCO3 accumulation was determined and liquid media. CaCO3 crystals were analyzed in the scanning electron microscope (SEM). Urease enzyme activities of the isolates were compared to with control group Sporosarcina pasteurii. According to results, F10 (Bacillus mycoides) was determined as species having the highest activity with specific urease activity 8.50±0.00 U/mg. The bacterium having the lowest specific activity was determined F14 (Bacillus thuringiensis) with specific urease activity 2.75±0.04 U/mg value. In liquid medium supplemented with urea and calcium chloride, the maximum CaCO3 accumulation was determined to be 0.53 g in S. pasteurii. After the control strain, the maximum CaCO3 accumulation was measured as 0.20g, 0.20 g and 0.18 g in F9, F13 and F8 isolates, respectively. According to the results of SEM examinations, it was determined that the isolates carried out calcite accumulation.}, number={1}, publisher={Erciyes Üniversitesi}, organization={Erciyes Üniversitesi}