@article{article_1096246, title={Evaluation of miR-145 and miR-146a as potential biomarkers for diagnosis of Myelodysplastic Syndrome}, journal={Acta Medica Nicomedia}, volume={5}, pages={61–66}, year={2022}, DOI={10.53446/actamednicomedia.1096246}, author={Süsgün, Seda and Baykara, Onur and Yücesan, Emrah and Kuru, R. Dilhan and Aslaneli Çakmak, Başak and Yabacı, Ayşegül and Öngören, Şeniz and Deviren, Ayhan and Tarkan Argüden, Yelda}, keywords={Miyelodisplastik sendrom, tanısal biyobelirteç, miR-146a, ROC eğrisi}, abstract={Objective: Myelodysplastic syndromes (MDS) are a group of heterogeneous hematopoietic stem cell disorders characterized by ineffective hematopoiesis, bone marrow dysplasia, and peripheral cytopenias. microRNAs (miRNAs) are small non-coding RNAs that play key roles in post-transcriptional regulation of gene expression and have been determined potential in disease diagnostics and therapeutics owing to their stability. Recent evidence suggests that haploinsufficiency of the miR-145 and miR-146a, encoded from 5q Common Deleted Region (CDR) may contribute to the phenotype in MDS. Although, interstitial del(5q) is the most common chromosomal abnormality in MDS, these findings are inconsistent in Turkish patients. Therefore, we aimed to investigate assess the diagnostic value of miR-145/miR-146a and their relation with del(5q) or monosomy 5 in MDS. Methods: In order to determine the association between del(5q) and expression miR-145/miR-146a, conventional cytogenetics (CC), FISH, and qRT-PCR methods were performed for 24 patients with MDS and 20 healthy individuals. Additionally, ROC curves were generated to evaluate putative diagnostic value of miRNAs. Results: Cytogenetic examination revealed clonal cytogenetic abnormalities in 43.4% of cases. miR-146a decreased in 23 of 24 patients regardless of chromosome 5 abnormalities (p <0.0001), expression level of miR-145 was statistically nonsignificant. miR-146a levels performed well as a diagnostic biomarker, discriminating MDS patients from controls with an area under the ROC curve (AUC) of 0.942, 83.3% sensitivity. Conclusion: miR-146a may be used as a biomarker in diagnosis of MDS and may help to identify new treatment targets. In addition, we suggest that CC and FISH methods should be performed together in MDS.}, number={2}, publisher={Kocaeli Üniversitesi}, organization={This work was supported by the grants of Scientific Research Projects Coordination Unit of Istanbul University (no: 24461) and Turkish Society of Hematology (no: 2017/1).}