@article{article_1109994, title={Cloning, Expression, and Purification of Acinetobacter baumannii OccAB1 Porin Protein in Escherichia coli}, journal={Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi}, volume={6}, pages={1529–1542}, year={2023}, author={Kaygusuz İzgördü, Özge and Darcan, Cihan and Doğan Guzel, Fatma}, keywords={E. coli, A. baumannii, Ni-NTA, Protein saflaştırma, OccAB1}, abstract={Acinetobacter baumannii is a free-living gram-negative organism in different habitats such as soil, water, surfaces of human and animal bodies. However, adapting to a pathogenic lifestyle over time, it causes infections and deaths in intensive care units all over the world. Porin proteins play a fundamental role in antibiotic resistance in this bacterium. Overexpression of porins and efflux pumps have been identified as contributing factors in increasing drug resistance and inactivation of therapy. For this reason, to study antibiotic transitions in vitro, these proteins must be purified first. With this porin protein obtained after purification, the passage of antibiotics in bilayer lipid layers will be investigated. In this study, A. baumannii OccAB1 porin protein was cloned and expressed in E. coli. As a result, A. baumannii OccAB1 porin cloned into the expression vector was successfully expressed in E. coli.}, number={2}, publisher={Osmaniye Korkut Ata Üniversitesi}, organization={TUBITAK}