TY  - JOUR
T1  - Therapeutic Effect of Sinapic Acid against 5-Fluorouracil-Induced Oxidative Stress and Inflammation in Rat Ovarium: An Experimental Approach
TT  - Sıçan Yumurtalık Dokusunda 5-Florourasil ile Uyarılan Oksidatif Stres ve İnflamasyona Karşı Sinapik Asitin Terapötik Etkisi: Deneysel Bir Yaklaşım
AU  - Demir, Selim
AU  - Mentese, Ahmet
AU  - Livaoglu, Ayten
AU  - Ayazoglu Demir, Elif
AU  - Türkmen Alemdar, Nihal
AU  - Aliyazıcıoğlu, Yüksel
PY  - 2023
DA  - June
DO  - 10.59518/farabimedj.1221397
JF  - Farabi Tıp Dergisi
JO  - Farabi Med J
PB  - Karadeniz Teknik Üniversitesi
WT  - DergiPark
SN  - 2979-9821
SP  - 1
EP  - 7
VL  - 2
IS  - 2
LA  - en
AB  - Tissue toxicity caused by 5-fluorouracil (5-FU) is associated with increased reactive oxygen species and inflammatory cytokines. Sinapic acid (SA) has both antioxidant and anti-inflammatory activities. Although SA has been shown to ameliorate chemical-induced tissue damage in various experimental models, its effects against 5-FU-induced ovarian damage have not yet been investigated. It was therefore aimed to evaluate the therapeutic potential of SA against 5-FU-induced ovarian damage in rats, together with the mechanisms of oxidative stress and inflammation for the first time in this study. Thirty rats were distributed into five groups: control, 5-FU (100 mg/kg) 5-FU+SA (2.5 and 5 mg/kg) and SA (5 mg/kg). 5-FU was applied to rats intraperitoneally on the 1st day of experiments and then SA was administrated for 3 successive days. Ovarian levels of lipid peroxidation [malondialdehyde (MDA)], oxidative stress (total oxidant status (TOS) and oxidative stress index (OSI)] antioxidant system [total antioxidant status (TAS), and catalase (CAT)], DNA damage [8-hydroxy-2'-deoxyguanosine (8-OHdG)] and inflammatory [interleukin-6 (IL-6)] markers in ovarian tissues were determined using spectrophotometric methods. It was determined that a single dose of 5-FU administration in rats significantly increased oxidative stress and inflammation in the ovarian tissue and suppressed the antioxidant system compared to the control group (p
KW  - 5-fluorouracil
KW  - Inflammation
KW  - Ovarian damage
KW  - Oxidative stress
KW  - Rat
KW  - Sinapic acid
N2  - 5-florourasil (5-FU)'nun neden olduğu doku toksisitesi, artan reaktif oksijen türleri ve inflamatuar sitokinler ile ilişkilidir. Sinapik asit (SA) bir fenolik asittir ve hem antioksidan hem de anti-inflamatuar aktivitelere sahiptir. Çeşitli deneysel modellerde SA'nın kimyasal kaynaklı doku hasarını iyileştirdiği gösterilmiş olmasına rağmen, 5-FU kaynaklı yumurtalık hasarına karşı etkileri henüz araştırılmamıştır. Bu nedenle bu çalışmada SA'nın sıçanlarda 5-FU ile indüklenen yumurtalık hasarına karşı terapötik potansiyelinin oksidatif stres ve inflamasyon mekanizmaları ile birlikte ilk kez değerlendirilmesi amaçlandı. Otuz sıçan beş gruba ayrıldı: kontrol, 5-FU (100 mg/kg), 5-FU+SA (2,5 ve 5 mg/kg) ve SA (5 mg/kg). Sıçanlara deneylerin 1. günü intraperitoneal yoldan 5-FU, ardından 3 gün boyunca SA uygulandı. Yumurtalık lipid peroksidasyon seviyeleri [malondialdehit (MDA)], oksidatif stres (toplam oksidan durum (TOS) ve oksidatif stres indeksi (OSI)], antioksidan sistem [toplam antioksidan durum (TAS) ve katalaz (CAT)], DNA hasarı [8-hidroksi-2'-deoksiguanozin (8-OHdG)] ve inflamatuvar [interlökin-6 (IL-6)] belirteçlerinin düzeyleri spektrofotometrik yöntemlerle belirlendi. Sıçanlarda tek doz 5-FU uygulamasının kontrol grubuna göre yumurtalık dokusunda oksidatif stresi ve inflamasyonu anlamlı olarak arttırdığı ve antioksidan sistemi baskıladığı belirlendi (p
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UR  - https://doi.org/10.59518/farabimedj.1221397
L1  - https://dergipark.org.tr/tr/download/article-file/2841224
ER  -