TY - JOUR T1 - Atomik kuvvet mikroskobu ve tarayıcı elektron mikroskobuyla timpanosklerotik plakların analizi TT - Effects of penicillin and montelukast sodium on middle ear mucosa in rats with experimental acute otitis media AU - Topalsan, Seçil Uçar AU - Aslan, Hale AU - Huseynov, Tural AU - Başoğlu, Mehmet Sinan AU - İlknur, Ali Ekber AU - Sarıoğlu, Sülen AU - Şerbetçioğlu, Bülent AU - Özbay, Can AU - Eren, Erdem AU - Oğuz Kılıç, Duygu AU - Kulduk, Erkan AU - Öztürkcan, Sedat AU - Katılmış, Hüseyin PY - 2012 DA - September DO - 10.2399/jmu.2012003003 JF - Journal of Medical Updates PB - Sürekli Eğitim ve Bilimsel Araştırmalar Derneği WT - DergiPark SN - 2146-3840 SP - 89 EP - 93 VL - 2 IS - 3 LA - tr AB - Amaç: Atomik kuvvet mikroskobu ile kalsiyum kürecikleri oluşturan timpanosklerotik plak oluşumunu görüntüleyerek literatüre yeni bilgiler sunmak ve tarayıcı elektron mikroskobuna monte edilmiş enerji yayıcı X-ışını dedektörü ile temel yapısını incelemek. Yöntem: Timpanoskleroz cerrahisi geçirmiş 30 hastadan alınan örnekler üçüncü basamak sevk merkezimizde geriye dönük olarak incelendi. Atomik kuvvet mikroskobuyla en sert plağın yüzey topografisi ve üç boyutlu görüntüleri analiz edildi ve tarayıcı elektron mikroskobu – enerji yayıcı X-ışını spektroskopisiyle 5 farklı plak içindeki 5 farklı kalsiyum küreciğinin temel bileşimi incelendi. Bulgular: Atomik kuvvet mikroskobuyla timpanosklerotik plağın üç boyutlu analizi kalsiyum fosfat kristalli oluşumları göstermiştir. Var olan kalsiyum, fosfat, karbon, nitrojen, oksijen, sodyum ve magnezyum miktarının tayini için enerji yayıcı X-ışını spektroskopisiyle tarayıcı elektron mikroskobu kullanılmıştır. Sonuç: Timpanosklerotik plağın temel bileşimi hakkında bilgi edinmek için yüzey topografisi kullanımının timpanosklerozun etiyolojisi ve tedavisini anlayışımıza katkıda bulunacağına inanmaktayız. KW - Timpanoskleroz KW - plak KW - analiz N2 - Objective: To develop an experimental acute suppurative otitis media model and compare the responses of rats to penicillin and combinations of leukotriene antagonist with respect to histopathological observations conducted at both early and late phases. Methods: A total of eighty-three ears from fifty six Wistar rats used in this study. Pneumococcus suspension was injected transtympanically to all the rats. Subjects were classified under four different groups having 14 rats at each. In Group A, intramuscular penicillin G was injected to rats for a period of five days. In Group B, intraperitoneal montelukast was injected for 21 days in addition to penicillin. In Group C, intraperitoneal montelukast and in Group D intraperitoneal isotonic NaCl was injected to rats for 21 days. Cross-sections were semi-quantitatively graded with respect to various inflammatory components. Results: No significant difference was found between the groups, apart from mucosal vascularization with respect to mucosal and tympanic membrane (TM) parameters at early phases. However, statistically significant differences were found for the improvement of TM thickness with the help of penicillin treatment. Furthermore, considerable deviations were observed for the recuperation of TM and mucosal inflammation for groups where subjects were injected with montekulast as compared to other groups of the study. Conclusion: The results of this study clearly show that the beneficial effects of the antibiotic (penicillin) as well as leukotriene antagonist (montelukast) is statistically different those of placebo in acute otitis media in rats. When the parameters of inflammation in the rat middle ear were compared with each other, most of these parameters did not show any statistically different beneficial effects in montelukast and penicillin groups. CR - Gibb AG. Tympanosclerosis. 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CR - This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY CR - NC-ND3.0) Licence (http://creativecommons.org/licenses/by-nc-nd/3.0/) which permits unrestricted noncommercial use, distribution, and reproduc CR - tion in any medium, provided the original work is properly cited. CR - Please cite this article as: Aslan H, Başoğlu MS, İlknur AE, Özbay C, Eren E, Oğuz Kılıç D, Kulduk E, Öztürkcan S, Katılmış H. Analysis of tym CR - panosclerotic plaques via atomic force microscope and scanning electron microscope. J Med Updates 2012;2(3):89-93. UR - https://doi.org/10.2399/jmu.2012003003 L1 - https://dergipark.org.tr/tr/download/article-file/105514 ER -