TY  - JOUR
T1  - Gümüş Nanopartiküllerin Morfolojisinin Protein Etkileşimleri Üzerindeki Etkisi
TT  - The Effect of the Morphology of Silver Nanoparticles on Their Interactions with Proteins
AU  - Öksel Karakuş, Ceyda
AU  - Tomak, Aysel
PY  - 2024
DA  - January
DO  - 10.21205/deufmd.2024267610
JF  - Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen ve Mühendislik Dergisi
JO  - DEUFMD
PB  - Dokuz Eylül Üniversitesi
WT  - DergiPark
SN  - 1302-9304
SP  - 82
EP  - 89
VL  - 26
IS  - 76
LA  - tr
AB  - Nanoteknolojideki gelişmelere paralel olarak biyomedikal uygulamalarda kullanılan nanopartiküllerin sayısında hızlı bir artış yaşanmıştır. Gümüş nanopartiküller, farklı metalik nanopartikül grupları arasında başta antibakteriyel etkinlik olmak üzere tıbbi uygulamaların gereksinimleriyle örtüşen çeşitli avantajlara sahip olmalari dolayısıyla öne çıkmakta ve yaygın olarak kullanılmaktadır. Gümüş nanopartikülleri avantajlı kılan yapı ve yüzey özellikleri biyolojik ortam etkileşimleri sonucunda değişiklik gösterebilmekte ve bu değişimler dolayısıyla biyolojik aktivite ve foksiyonellik gibi nanopartikül özellikleri de doğrudan etkilenmektedir. Nanopartiküllerin biyolojik ortamlarda değişen yüzey özelliklerinin en büyük nedeninin yüzeylerine tutunan proteinler olduğu bilinmektedir. Ancak nanopartiküllerin morfolojik özelliklerinin etraflarında oluşan bu protein halkasının bileşimine ve miktarına olan etkisi tam olarak aydınlatılmamıştır. Bu çalışmada, partikül morfolojisinin nanopartikül-protein etkileşimleri üzerine etkisi incelenmiştir. Bu amaçla küresel ve prizma-benzeri yapıya sahip gümüş nanopartikülleri detaylı olarak karakterize edilmiş ve yüzeylerine tutunan proteinler sodyum dodesil sülfat–poliakrilamid jel elektroforezi (SDS–PAGE) yöntemiyle analitik olarak tayin edilmiştir. Spesifik olarak, küresel ve prizmatik morfolojiye sahip gümüş nanopartikülleri protein eklentili hücre kültürü ortamı içerisinde farklı süre (15 dk, 2 sa ve 24 sa) ve sıcaklıklarda (22 oC ve 37 oC) inkübe edilmiş ve yüzeylerine tutunan proteinler tür ve miktar açısından karşılaştırılmıştır.
KW  - Nanopartikül
KW  - Nanogümüş
KW  - Protein Korona
KW  - Jel Elektroforezi
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UR  - https://doi.org/10.21205/deufmd.2024267610
L1  - http://dergipark.org.tr/tr/download/article-file/2946050
ER  -