TY  - JOUR
T1  - Evaluation of the Antiproliferative Effect of Safranal in C-4 I Cervical Cancer Cell Line
TT  - Safranal'ın Servikal Kanseri C-4 I Hücre Hattı Üzerindeki Antiproliferatif Etkisinin Değerlendirilmesi
AU  - Athoumanı Alı, Souandaou
AU  - Özcan, Fatma Gül
AU  - Karabulut-bulan, Omur
PY  - 2023
DA  - October
DO  - 10.59312/ebshealth.1367528
JF  - Doğu Karadeniz Sağlık Bilimleri Dergisi
JO  - EBSHealth
PB  - Giresun Üniversitesi
WT  - DergiPark
SN  - 2822-6445
SP  - 117
EP  - 128
VL  - 2
IS  - Kongre Özel Sayısı
LA  - en
AB  - Aim: Safranal is a bioactive compound responsible for the aroma propriety of Crocus sativus. Many studies have shown the antioxidant activity of safranal besides some pharmacological properties, including its anti-inflammatory effect. Our study aimed on identifying the cytotoxic effects of safranal on the C-4 I cell line of cervical cancer. Methods: The cytotoxic effect of safranal on the C-4 I cell line was determined after incubating the cells for certain durations (2 to 72 hours) and concentrations (25 to 800 µM). After incubation, cell viability and anti-proliferation effect of safranal were determined respectively by Mitochondrial Dehydrogenase and Lactate dehydrogenase enzymes activities assays. Additionally, morphological changes occurring during incubation in cells were examined with inverted and optical microscope using Giemsa staining. Results: According to the results, compared to Control group, the % viability of treated cells was decreased depending on concentration and the incubation time, and safranal significantly inhibited the growth of C-4 I cells (p
KW  - C-4 I
KW  - Safranal
KW  - Cervical cancer
KW  - Cytotoxicity
KW  - Antiproliferative
N2  - Amaç: Safranal, Crocus sativus’un aroma özelliğinden sorumlu bioaktif bir bileşendir. Safranalın antiinflamatuar etkisi dahil olmak üzere farmakolojik özelliklerinin yanı sıra antioksidan aktivitesi birçok çalışmada gösterilmiştir. Çalışmamızda, safranalın serviks karsınoma kökenli C-4 I hücre hattı üzerindeki sitotoksik etkilerini tespit etmek amaçlanmıştır. Yöntem: Safranalın C-4 I hücre hattı üzerindeki sitotoksik etkisini belirlemek için hücreler belirli sürelerde (2-72 saat) ve konsantrasyonlarda (25-800μM) inkübe edildi. İnkübasyon süresi bitiminde hücrelerin viabilitesi ve safranal antiproliferatif etkisi sırasıyla Mitokondriyal Dehidrojenaz ve Laktat dehidrojenaz enzimlerinin aktiviteleri ile belirlendi. Bununla birlikte, inkübasyon süresince meydana gelen morfolojik değişiklikler Faz kontrast ve ışık mikroskopları aracılığıyla Giemsa boyama kullanılarak gözlemlendi. Bulgular: elde edilen sonuçlara göre, safranal ile tedavi edilen hücreler kontrol grubu ile karşılaştırıldığında hücrelerin canlılık yüzdesi doza ve inkübasyon süresine bağlı olarak azaldı ve safranal C-4 I hücrelerinin çoğalmasını anlamlı bir şekilde inhibe etti (p
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UR  - https://doi.org/10.59312/ebshealth.1367528
L1  - https://dergipark.org.tr/tr/download/article-file/3438681
ER  -