TY  - JOUR
T1  - Histopathological and Antioxidant Effects of Bromelain on Kidney Tissue of Tyloxapol-Induced Hyperlipidemic Rats
TT  - Tyloxapol ile İndüklenmiş Hiperlipidemik Sıçanların Böbrek Dokusu Üzerinde Bromelainin Histopatolojik ve Antioksidan Etkileri
AU  - Palabıyık, Esra
AU  - Sulumer, Ayşe Nurseli
AU  - Avcı, Bahri
AU  - Uğuz, Handan
AU  - Aşkın, Hakan
PY  - 2024
DA  - August
Y2  - 2024
DO  - 10.53433/yyufbed.1381717
JF  - Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi
JO  - YYUFBED
PB  - Van Yüzüncü Yıl Üniversitesi
WT  - DergiPark
SN  - 1300-5413
SP  - 413
EP  - 422
VL  - 29
IS  - 2
LA  - en
AB  - This study was designed to investigate the nephroprotective effect of Bromelain against oxidative stress induced by Tyloxopol-induced hyperlipidemia in rats. Rats (n=6) were divided into three groups. I: Control (C) Group: No treatment was given and only distilled water was given; II: Tyloxapol (TX) Group: Group receiving Tyloxapol (400 mg/kg, i.p); III: Tyloxapol + Bromelain (TX+BR) Group: Group receiving Tyloxapol (400 mg/kg, i.p.) and group receiving Bromelain (250 mg/kg, o.d.) for 18 days. As a result of Tyloxapol administration, oxidative stress parameters SOD and CAT levels decreased, while MDA levels increased. In addition, histopathologic variations were detected in kidney sections. On the other hand, lipid peroxidation decreased and most of the studied parameters improved with combined administration (TX+BR group). According to the results obtained, bromelain may be a potent and alternative agent with protective effect for further studies.
KW  - Bromelain
KW  - Histopathology
KW  - Hyperlipidemia
KW  - Kidney
KW  - Oxidative stress
KW  - Tyloxapol
N2  - Bu çalışma, ratlarda Tyloxopol ile indüklenen hiperlipidemi neticesinde oluşan oksidatif strese karşı Bromelain’in nefroprotektif etkisinin incelenmesi için tasarlandı. Ratlar (n=6) üç grup altında sınıflandırıldı. I: Kontrol (C) Grubu: Herhangi bir uygulama yapılmayan ve sadece distile su verilen grup; II: Tyloxapol (TX) Grup: Tylaxopol (400 mg/kg, i.p) alan grup; III: Tyloxapol + Bromelain (TX+BR) Grup: Tylaxopol (400 mg/kg, i.p) alan ve 18 gün Bromelain (250 mg/kg, o.d.) alan grup. Tyloxapol uygulaması neticesinde oksidatif stres parametrelerinden SOD ve CAT seviyelerinde azalma meydana gelirken MDA seviyelerinde artış görüldü.  Buna ek olarak böbrek kesitlerinde histopatolojik varyasyonlar tespit edildi. Bunun aksine çalışmada, kombine uygulama ile (TX+BR grubu) lipid peroksidasyonu azaldı ve çalışılan parametrelerin çoğunda iyileşme görüldü. Elde edilen sonuçlara göre bromelain’in daha sonraki çalışmalar için koruyucu etkiye sahip güçlü ve alternatif bir ajan olabileceği düşünülmektedir.
CR  - Akaras, N., Toktay, E., Celep, N. A., Yüce, N., Şimşek, H., & Özkan, H. İ. (2023). Antioxidant effects of bromelain on paracetamol-induced renal injury in rats. Archives of Basic Clinical Research, 5, 364-371. https://doi.org/10.5152/ABCR.2023.22123
CR  - Akinmoladun, A. C., Adegbamigbe, A. D., Okafor, N. R., Josiah, S. S., & Olaleye, M. T. (2021). Toxicological and pharmacological assessment of a multi herbal phytopharmaceutical on Triton X-1339-induced hyperlipidemia and allied biochemical dysfunctions. Journal of Food Biochemistry, 45(3), e13238. https://doi.org/10.1111/jfbc.13238
CR  - Alissa, E. M., & Ferns, G. A. (2017). Dietary fruits and vegetables and cardiovascular diseases risk. Critical Reviews in Food Science and Nutrition, 57(9), 1950-1962. https://doi.org/10.1080/10408398.2015.1040487
CR  - Allawadhi, P., Khurana, A., Sayed, N., Kumari, P., & Godugu, C. (2018). Isoproterenol‐induced cardiac ischemia and fibrosis: Plant‐based approaches for intervention. Phytotherapy Research, 32(10), 1908-1932. https://doi.org/10.1002/ptr.6152
CR  - Baldissera, M. D., Souza, C. F., Grando, T. H., Doleski, P. H., Boligon, A. A., Stefani, L. M., & Monteiro, S. G. (2017). Hypolipidemic effect of β-caryophyllene to treat hyperlipidemicrats. Naunyn-Schmiedeberg's Archives of Pharmacology, 390(2), 215-223. https://doi.org/10.1007/s00210-016-1326-3
CR  - Chukwuebuka, N. B., Elias, D. T. M., Ijego, A. E., Peggy, O. E., Ejime, A. C., & Omeru, O. (2021). Changes in antioxidant enzymes activities and lipid peroxidase level in tissues of stress-induced rats. Biomedical and Pharmacology Journal, 14(2), 583-596. https://dx.doi.org/10.13005/bpj/2161
CR  - Colletti, A., Li, S., Marengo, M., Adinolfi, S., & Cravotto, G. (2021). Recent advances and insights into bromelain processing, pharmacokinetics and therapeutic uses. Applied Sciences, 11(18), 8428. https://doi.org/10.3390/app11188428
CR  - Çakmak, Ö. (2020). The effects of boric acid on renal ischemia/reperfusion injury in rat. (MSc), Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Erzurum, Türkiye.
CR  - deSousa, J. A., Pereira, P., Allgayer, M. D. C., Marroni, N. P., de Barros Falcão Ferraz, A., & Picada, J. N. (2017). Evaluation of DNA damage in Wistar rat tissues with hyperlipidemia induced by tyloxapol. Experimental and Molecular Pathology, 103(1), 51–55. https://doi.org/10.1016/j.yexmp.2017.06.009
CR  - Dudhipala, N. R., Ettireddy, S. R., & Puchakayala, G. R. (2021). Attenuation of lipid levels in triton induced hyperlipidemia rats through rosuvastatin calcium nanoparticles: Pharmacokinetic and pharmacodynamic studies. Chemistry and Physics of Lipids, 237, 105081. https://doi.org/10.1016/j.chemphyslip.2021.105081
CR  - El-Demerdash, F. M., Baghdadi, H. H., Ghanem, N. F., & Al Mhanna, A. B. (2020). Nephroprotective role of bromelain against oxidative injury induced by aluminum in rats. Environmental Toxicologyand Pharmacology, 80, 103509. https://doi.org/10.1016/j.etap.2020.103509
CR  - Feng, Y., Gao, S., Zhu, T., Sun, G., Zhang, P., Huang, Y., Qu, S., Du, X., & Mou, D. (2022). Hawthorn fruit acid consumption attenuate shyperlipidemia-associated oxidative damage in rats. Frontiers in Nutrition, 9, 936229. https://doi.org/10.3389/fnut.2022.936229
CR  - Fidèle, N., Joseph, B., Emmanuel, T., & Théophile, D. (2017). Hypolipidemic, antioxidantand anti-atherosclerogenic effect of aqueous extract leaves of Cassia. Occidentalis Linn (Caesalpiniaceae) in diet-induced hypercholesterolemic rats. BMC Complementary and Alternative Medicine, 17(1), 76. https://doi.org/10.1186/s12906-017-1566-x
CR  - Gai, Z., Wang, T., Visentin, M., Kullak-Ublick, G. A., Fu, X., & Wang, Z. (2019). Lipid accumulation and chronic kidney disease. Nutrients, 11(4), 722. https://doi.org/10.3390/nu11040722
CR  - Gara, A. B., Kolsi, R. B. A., Chaaben, R., Hammami, N., Kammoun, M., Patti, F. P., ... & Belghith, K. (2017). Inhibition of key digestive enzymes related to hyperlipidemia and protection of liver-kidney functions by Cystoseira crinita sulphated polysaccharide in high-fat diet-fed rats. Biomedicine & Pharmacotherapy, 85, 517-526. https://doi.org/10.1016/j.biopha.2016.11.059
CR  - Gaschler, M. M., & Stockwell, B. R. (2017). Lipid peroxidation in celldeath. Biochemical and Biophysical Research Communications, 482(3), 419-425. https://doi.org/10.1016/j.bbrc.2016.10.086
CR  - Gürel, A., & Kaya, K. (2022). Bromelain has antioxidant effect on methotrexate hepatotoxicity and nephrotoxicity. Van Sağlık Bilimleri Dergisi, 15(1), 37-42. https://doi.org/10.52976/vansaglik.982411
CR  - Hikisz, P., & Bernasinska-Slomczewska, J. (2021). Beneficial properties of Bromelain. Nutrients, 13(12), 4313. https://doi.org/10.3390/nu13124313
CR  - Husain, M. J., Spencer, G., Nugent, R., Kostova, D., & Richter, P. (2022). The cost-effectiveness of hyperlipidemia medication in low- and middle-income countries: a review. Global Heart, 17(1), 18. https://doi.org/10.5334/gh.1097
CR  - Iyer, D., & Patil, U. K. (2019). Assessment of Antihyperlipidemic and Antitumor effect of isolated active Phytoconstituents from Apium graveolens L. through Bioassay-Guided procedures. Journal of Dietary Supplements, 16(2), 193-206. https://doi.org/10.1080/19390211.2018.1448921
CR  - Kalaiselvi, M., Gomathi, D., Ravikumar, G., Devaki, K., & Uma, C. (2013). Ameliorative effect of Ananus comosus peel on 7, 12 dimethylbenz (α) anthracene induced mammary carcinogenesis with reference tooxidative stress. Journal of Acute Disease, 2(1), 22-28. https://doi.org/10.1016/S2221-6189(13)60089-X
CR  - Karale, P., Dhawale, S., & Karale, M. (2022). HR-LCMS analysis and Antihyperlipidemic effect of Ethanolic Leaf extract of Momordica charantia L. Hacettepe University Journal of the Faculty of Pharmacy, 42(2), 93-104. https://doi.org/10.52794/hujpharm.1063583
CR  - Li, Y., Gong, W., Liu, J., Chen, X., Suo, Y., Yang, H., & Gao, X. (2022). Angiopoietin-like protein 4 promotes hyperlipidemia-induced renal injurybydown-regulatingtheexpression of ACTN4. Biochemical and Biophysical Research Communications, 595, 69-75. https://doi.org/10.1016/j.bbrc.2022.01.061
CR  - Liu, Y., Sun, Y., Xue, B. H., Wang, X. D., & Yu, W. L. (2021). Negative regulation of SIRT1 by IRF9 ınvolved in Hyperlipidemia acute Pancreatitis associated with Kidney ınjury. Digestive Diseases and Sciences, 66(4), 1063-1071. https://doi.org/10.1007/s10620-020-06331-1
CR  - Manzoni, A. G., Passos, D. F., Leitemperger, J. W., Storck, T. R., Doleski, P. H., Jantsch, M. H., ... & Leal, D. B. (2020). Hyperlipidemia-induced lipotoxicity and immune activation in rats are prevented by curcumin and rutin. International Immunopharmacology, 81, 106217. https://doi.org/10.1016/j.intimp.2020.106217
CR  - Nwozo, S. O., Lewis, Y. T., & Oyinloye, B. E. (2017). The effects of Piper Guineense versus Sesamum Indicum Aqueous extracts on Lipid metabolism and antioxidants in hypercholesterolemic rats. Iranian Journal of Medical Sciences, 42(5), 449-456.
CR  - Palabiyik, E., Sulumer, A. N., Uguz, H., Avci, B., Askin, S., & Askin, H. (2023). Walnut fruit diaphragm ethanol extract ameliorates damage due to Triton WR‐1339‐induced hyperlipidemia in rats. European Journal of Lipid Science and Technology, 2300105. https://doi.org/10.1002/ejlt.202300105
CR  - Parwin, A., Najmi, A. K., Ismail, M. V., Kaundal, M., & Akhtar, M. (2019). Protective effects of alendronate in Triton X-100-induced hyperlipidemia in rats. The Turkish Journal of Gastroenterology, 30(6), 557-564. https://doi.org/10.5152/tjg.2019.18076
CR  - Singh, R. P., & Pattnaik, A. K. (2024). Scientific insights into Hyperlipidemia Mitigation: a profound examination of ısolated bioactive fractions of Kalanchoe pinnata (Lam.) leaves and their therapeutic implications using In Vitro, In Vivo, and In Silico study from the characterized compounds using HPTLC MS/MSn analysis. Pharmacognosy Magazine, (online first). https://doi.org/10.1177/09731296241228925
CR  - Sulumer, A. N., Palabıyık, E., Avcı, B., Uguz, H., Demir, Y., Serhat Özaslan, M., & Aşkın, H. (2023). Protective effect of bromelain on some metabolic enzyme activities in tyloxapol‐induced hyperlipidemic rats. Biotechnology and Applied Biochemistry. https://doi.org/10.1002/bab.2517
CR  - Toth, P. P., & Banach, M. (2019). Statins: then and now. Methodist DeBakey cardiovascular journal, 15(1), 23-31. https://doi.org/10.14797/mdcj-15-1-23
UR  - https://doi.org/10.53433/yyufbed.1381717
L1  - https://dergipark.org.tr/tr/download/article-file/3498725
ER  -