TY  - JOUR
T1  - ANALYSIS OF VOLATILE COMPOUNDS OF ALCEA CALVERTII BOISS. AND ITS ANTIMICROBIAL, ANTICHOLINESTERASE, AND ANTITYROSINASE POTENCY
TT  - ALCEA CALVERTII BOİSS.’İN UÇUCU BİLEŞENLERİNİN ANALİZİ ANTİMİKROBİYAL, ANTİKOLİNESTERAZ VE ANTİTİROZİNAZ ETKİSİ
AU  - Sener, Sıla Özlem
AU  - Kanbolat, Şeyda
AU  - Ulaş Çolak, Nevin
AU  - Badem, Merve
AU  - Aliyazıcıoğlu, Rezzan
AU  - Özgen, Ufuk
AU  - Alpay Karaoğlu, Şengül
AU  - Kandemir, Ali
PY  - 2024
DA  - July
Y2  - 2024
DO  - 10.34108/eujhs.1384325
JF  - Sağlık Bilimleri Dergisi
JO  - JHS
PB  - Erciyes Üniversitesi
WT  - DergiPark
SN  - 1018-3655
SP  - 190
EP  - 198
VL  - 33
IS  - 2
LA  - en
AB  - The research aimed to analyze the volatile compounds by using SPME-GC-MS and to detect anticholinesterase, antityrosinase, and antimicrobial potential of metanol extract of Alceacalvertii Boiss., an endemic species for Türkiye. The inhibitory effects of tyrosinase, acetylcholinesterase, and butyrylcholinesterase of the plant were determined by spectroscopic technique and the plant’s antimicrobial activity was assessed using the agar diffusion method. A total of 18 volatile compounds were specified belonging to terpenes classes. o-cymene (10.60%) and sesquicineole (15.55%) were detected as major volatile components of the species by the SPME-GC-MS technique. Meaningful antimicrobial activity was observed on Candida tropicalis, Enterococcus faecalis, Escherichia coli, Mycobacterium smegmatis, Staphylococcus aureus, and Pseudomonas aeruginosa. The half maximal inhibitory concentration (IC50) value of the plant was determined 60.12 ± 1.75 μg/mL as a result of tyrosinase assay. IC50 value was found 111.54 ± 1.75 μg/mL, according to butyrylcholinesterase inhibition studies. Considering all the findings, it has been determined that the plant includes diverse volatile compounds and showed promising antimicrobial, tyrosinase inhibitory, and moderate butyrylcholinesterase inhibitory effects so Alceacalvertii may be the up-and-coming source of natural medicine for microbial and dermatological diseases although limited to its effects for Alzheimer's disease.
KW  - Alcea calvertii Boiss.
KW  - Anticholinesterase
KW  - Antimicrobial
KW  - Antityrosinase
KW  - GC-MS
N2  - Bu araştırmanın amacı Türkiye için endemic bir tür olan Alceacalvertii Boiss’in uçucu bileşenlerin SPME-GC-MS kullanılarak analiz edilmesi ve bitkinin methanol ekstresinin antikolinesteraz, antitirozinaz ve antimikrobiyal potansiyelinin belirlenmesidir. Türün tirozinaz, asetilkolinesteraz ve bütirilkolinesterazin hibitör etkileri spektroskopik teknikle belirlenmiş olup, antimikrobiyal aktivitesi için agar difüzyon yöntemi kullanılmıştır. Çalışma sonucunda terpenler sınıfı ait toplam 18 uçucu bileşen belirlenmiştir. SPME-GC-MS tekniği ile belirlenen uçucu bileşenelerinden o-simen (%10.60) ve seskisinol (%15.55) türün ana uçucu bileşenleri olarak tespit edilmiştir. Candida tropikalis, Escherichia coli, Enterococcus faecalis, Mycobacterium smegmatis, Pseudomonas aeruginosa ve Staphylococcus aureus üzerinde ekstrenin anlamlı antimikrobiyal aktivite gösterdiği gözlemlenmiştir. Antitirozinaz aktivite çalışmaları sonucunda ekstrenin yarı maksimum inhibisyon konsantrasyonu (IC50) değeri 60.12 ± 1.75 µg/mL olarak belirlenmiştir. Bütirilkolinesteraz inhibisyon çalışmalarına göre IC50 değeri 111.54 ± 1.75 µg/mL olarak tespit edilmiştir. Tüm bulgular gözönüne alındığında, türün zengin uçucu bileşen içeriğine sahip olduğu ve ümit verici antimikrobiyal, tirozinazinhibitör ve orta derecede bütirilkolinesteraz inhibitor etkiler gösterdiği, dolayısıyla Alceacalvertii’nin Alzheimer hastalığı için tedavi edici değeri sınırlı olmakla birlikte mikrobiyal, dermatolojik ve gelecek vaat eden doğal ilaç kaynağı olabileceği değerlendirilmiştir.
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UR  - https://doi.org/10.34108/eujhs.1384325
L1  - https://dergipark.org.tr/tr/download/article-file/3509347
ER  -