TY  - JOUR
T1  - Obezite Araştırmaları İçin İn Vitro Adiposit Hücre Kültürü İle Etkili Model Oluşturma: Ön Çalışma
TT  - Creating An Effective Model with In Vitro Adipocyte Cell Culture for Obesity Research: Preliminary Study
AU  - Soysal, Yasemin
AU  - Çil, Ezgi Nur
PY  - 2024
DA  - April
Y2  - 2024
DO  - 10.25048/tudod.1418459
JF  - Turkish Journal of Diabetes and Obesity
JO  - Turk J Diab Obes
PB  - Zonguldak Bülent Ecevit Üniversitesi
WT  - DergiPark
SN  - 2587-0335
SP  - 59
EP  - 64
VL  - 8
IS  - 1
LA  - tr
AB  - Amaç: 3T3-L1 preadipositlerin farklılaşma etkinliği obezite, diyabet ve ilgili bozukluklarla ilişkili hücresel mekanizmalar üzerine yapılançalışmaları etkileyen önemli bir faktördür. 3T3-L1 hücrelerinin farklılaşması genellikle insülin, deksametazon (DEX) ve 3-izobütil-1-metilksantin (IBMX) içeren bir adipojenik kokteyl ile indüklenmektedir. 3T3-L1 hücreleri çoğaltılırken dikkat edilmesi gerekenönemli noktalar içeren bir sürece sahip hassas bir hücre grubu olup ilerleyen pasajlarda farklılaşma yeteneğini kaybedebilmektedir. Busorunu çözmek için, çalışmamızın amacı doğrultusunda, yaygın olarak kullanılan üç farklı adipojenik kokteylin farklılaşma verimliliğikarşılaştırılmıştır. Bununla birlikte, birtakım parametreler değiştirilerek 3T3-L1 hücrelerinin farklılaşma yetenekleri test edilmiştir.Gereç ve Yöntemler: Çalışmamızda 3T3-L1 hücre kültürü, fibroblast hücrelerin olgun adiposit hücrelere farklılaştırılması, Oil Red Oboyama ile lipitlerin mikroskop altında gösterilmesi ve kantitatif analiz için boyanın spektrofotometrik ölçümü yöntemleri kullanılmıştır.Bulgular: Deney sonuçları, 0,5 mM IBMX, 0,25 μM DEX ve 1 μg/mL insülin içeren adipogenik kokteylin 3T3-L1 hücre farklılaşmasıiçin en etkili kokteyl olduğunu (p
KW  - Obezite
KW  - hücre kültürü
KW  - 3T3-L1
KW  - adipogenez
KW  - adiposit
N2  - Aim: The differentiation efficiency of 3T3-L1 preadipocytes is a crucial factor affecting studies on cellular mechanisms associated withobesity, diabetes and related disorders. Differentiation of 3T3-L1 cells is usually induced by an Adipogenic cocktail containing insulin,dexamethasone (DEX), and 3-isobutyl-1-methylxanthine (IBMX). There is a process that includes important points to consider whengrowing 3T3-L1 cells. It is a sensitive cell group and may löse its ability to differentiate in later passages. To solve this problem, for thepurpose of our study, the differentiation efficiency of three different commonly used Adipogenic cocktails was compared. Additionally,the differentiation abilities of 3T3-L1 cells were tested by changing some parameters.Material and Methods: In our study, 3T3-L1 cell culture, differentiation of fibroblast cells into mature adipocyte cells, demonstration oflipids under the microscope with Oil Red O staining, and spectrophotometric measurement of the dye for quantitative analysis were used.Results: Experimental results Showed that the Adipogenic cocktail containing 0.5 mM IBMX, 0.25 μM DEX and 1 μg/mL insülin wasthe most effective cocktail for 3T3-L1 cell differentiation (p
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UR  - https://doi.org/10.25048/tudod.1418459
L1  - https://dergipark.org.tr/tr/download/article-file/3654996
ER  -