@article{article_1432585, title={Determination of Total Phenolic Compounds and Antioxidant Activity of Turkish Propolis Extracted by Different Methods}, journal={ITU Journal of Food Science and Technology}, volume={2}, pages={41–46}, year={2024}, author={Sonverdi, Feride and Altuntaş, Ümit and Özçelik, Beraat}, keywords={Propolis, fenolik madde, ekstraksiyon, antioksidan aktivite, ultrason}, abstract={Propolis, also known as bee glue, has a wide range of biological activities and physiological properties. Propolis is a product collected by bees during honey making, and due to its resinous structure it cannot be consumed in its raw form and can be made suitable for consumption by separating unwanted substances. Many methods can be used to extract bioactive compounds from raw propolis, but since there may be differences between these methods in terms of extraction efficiency, an effective method should be used. In this study, the total phenolic content (TPC) and antioxidant activity of propolis samples extracted by microwave assisted ethanolic extraction (MAE), ultrasonic assisted ethanolic extraction (UAE), and supercritical fluid (SCF) extraction methods were investigated. In the experiments, TPC was analyzed by Folin-Ciocalteu method and antioxidant activity tests were performed using DPPH radical scavenging and CUPRAC methods. As a result of the analyses, the TPC of the samples was found in the range of 42.83-83.88 mg GAE/g sample, DPPH radical scavenging activity was in the range of 22.97-37.30 mg TE/g sample and antioxidant capacity obtained by CUPRAC method was in the range of 143.83-259.69 mg TE/g sample. In line with the values obtained, even though MAE and UAE were determined to have the highest phenolic content and antioxidant capacity among the propolis extracts, no significant difference was found between them, but as a result of the literature information, it was understood that the content of propolis varies according to climatic conditions and geographical origin and that MAE and UAE can be preferred in order to increase the extraction efficiency, while the procedures of the methods should be given more importance.}, number={1}, publisher={İstanbul Teknik Üniversitesi}