TY - JOUR T1 - Investigation of Mobilized Colistin Resistance Gene-1 in Poultry Pathogenic Escherichia coli AU - Türkyılmaz, Süheyla AU - Yilmaz, Ecenur PY - 2024 DA - December Y2 - 2024 DO - 10.53913/aduveterinary.1469963 JF - Animal Health Production and Hygiene JO - Animal Health, Prod and Hyg PB - Aydın Adnan Menderes Üniversitesi WT - DergiPark SN - 2146-7269 SP - 1 EP - 8 VL - 13 IS - 2 LA - en AB - This study aimed to determine the presence of mobilized colistin resistance gene 1 (mcr-1), which provides plasmid-mediated colistin resistance in avian pathogenic Escherichia coli (APEC) isolates, and to examine the antibiotic resistance profiles of colistin resistant isolates. In this research, 200 broiler liver samples suspected of colibacillosis were utilized as the material. Following the isolation of E. coli through classical conventional methods, identification and antibiotic susceptibility tests were conducted using an automated microbiology system (BD Phoenix 100TM, USA). The presence of the mcr-1 gene in isolates phenotypically determined as colistin resistant was investigated using polymerase chain reaction (PCR). Out of 156 (78%) E. coli isolates obtained from 200 broiler liver samples, 10 (6.4%) were phenotypically determined to be colistin resistant. It was found that 80% of colistin resistant E. coli isolates were resistant to levofloxacin, 70% to cefazolin and cefuroxime, 60% to ceftazidime, 50% to gentamicin and ceftriaxone, 40% to cefepime, 30% to ceftolozane-tazobactam, and 20% to piperacillin-tazobactam. All isolates were sensitive to amikacin, ertapenem, imipenem, meropenem, and resistant to ampicillin, amoxicillin-clavulanate, ampicillin-sulbactam, trimethoprim-sulfamethoxazole, and tigecycline, exhibiting multidrug resistance (MDR). All isolates that were found to be phenotypically resistant to colistin were found to carry the mcr-1 gene. These findings indicate that the mcr in APEC contributes to the rapid spread of plasmid-mediated resistance genes and the escalation of broad-spectrum antibiotic resistance. The detection of resistance to antibiotics used in human medicine may be poses a potential threat to public health. Future studies should be conducted with samples from different regions and include a diverse sample group to better understand this risk. KW - Avian pathogenic Escherichia coli KW - mobile colistin resistance gene KW - antibiotic resistance CR - Adıguzel, M.C., Baran, A., Wu, Z., Cengiz, S., Dai, L., Oz, C., Ozmenli, E., Goulart, B., & Sahin, O. (2021). Prevalence of colistin resistance in Escherichia coli in eastern Turkey and genomic characterization of an mcr-1 positive strain from retail chicken meat. Microbial Drug Resistance, 27(3), 424-432. https://doi.org/10.1089/mdr.2020.0209 CR - Apostolakos, I., & Piccirillo A. (2018). 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