TY - JOUR T1 - Schiff bazı içeren çinko(II) kompleksinin kolorektal kanser hücreleri üzerindeki antikanser aktivitesinin incelenmesi TT - Investigation of the anticancer activity of zinc(II) complex containing Schiff base on colorectal cancer cell AU - Uçar, Asuman AU - Çelik, Sultan AU - Pehlivanoğlu, Suray PY - 2024 DA - December Y2 - 2024 DO - 10.17714/gumusfenbil.1486484 JF - Gümüşhane Üniversitesi Fen Bilimleri Dergisi PB - Gümüşhane Üniversitesi WT - DergiPark SN - 2146-538X SP - 1039 EP - 1048 VL - 14 IS - 4 LA - tr AB - Kolorektal kanser, Dünya’da sık görülen ve kansere bağlı ölümler açısından ikinci sırada yer alan, tedavisi zor heterojen bir hastalıktır. Hastaların çoğunluğu tanı anında ileri evrededir ve kullanılan kemoterapötik ajanlara direnç söz konudur. Bu çalışmada potansiyel bir ajan olarak, 1,3-diaminopropan ve 2-hidroksi-1-naftaldehitten yola çıkarak N,N'-bis[(2-hidroksi-1- naftalenil)metilen]-propan-1,3-diamin ligandı (H2L) ve onun Zn(II) kompleksi sentezlenmiştir. Bileşikler 1H-NMR, 13C-NMR, FT-IR, UV ile karakterize edilmiştir ve HT29 kolorektal kanser hücreleri üzerine olan olası terapötik etkileri araştırılmıştır. H2L ve Zn(II) kompleksinin HT29 hücre canlılığı üzerine etkileri MTT testi ile değerlendirilmiştir. H2L ve Zn(II) kompleksinin HT29 hücrelerinde IC50 değerleri sırasıyla 117.1 µg/ml ve 17.03 µg/ml olarak hesaplanmıştır. Zn(II) kompleksi ligandına kıyasla hücre canlılığını anlamlı derecede azaltmış ve hücresel apoptotik morfolojilerin oluşmasını tetiklemiştir. Çalışmanın sonuçlarına göre, Zn(II) kompleksi umut veren ve terapötik potansiyeli olan bir kemoterapötik olabileceğini destekler niteliktedir. Gelecekte Zn(II) kompleksinin kolorektal kanser hücreleri üzerindeki etki mekanizmasını aydınlatacak daha kapsamlı çalışmalara ihtiyaç vardır. KW - Kolorektal kanser KW - Schiff bazı KW - Sitotoksisite KW - Zn(II) kompleksi N2 - Colorectal cancer is a common heterogeneous disease worldwide and is difficult to treat and ranks second in terms of cancer-related deaths. The majority of cases are at an advanced stage at the time of diagnosis and occurs resistance to the conventional chemotherapeutic agents. In this study, N,N'-bis[(2-hydroxy-1-naphthalenyl)methylene]-propane-1,3 diamine ligand (H2L) and its Zn(II) complex were synthesized as a potential agent, starting from 1,3-diaminopropane and 2-hydroxy-1-naphthaldehyde. The compounds were characterized by 1H-NMR, 13C-NMR, FT-IR, UV and their possible therapeutic effects on HT29 colorectal cancer cells were investigated. The effects of H2L and Zn(II) complex on HT29 cell viability were evaluated by MTT assay. IC50 values of H2L and Zn(II) complex on HT29 cells were calculated as 117.1 µg/ml and 17.03 µg/ml, respectively. Compared to its ligand, the Zn(II) complex significantly reduced viability and promoted apoptotic morphologies in the cells. According to the results of this study, Zn(II) complex may be a promising chemotherapeutic with therapeutic potential. More comprehensive studies are needed in the future to elucidate the mechanism of action of the Zn(II) complex on colorectal cancer cells. CR - Al-Hawarin, J. I., Abu-Yamin, A. A., Abu-Saleh, A. A. A. A., Saraireh, I. A. M., Almatarneh, M. H., Hasan, M., Atrooz, O. M., & Al-Douri, Y. (2023). Materials, 16(14), 5160. https://doi.org/10.3390/ma16145160 CR - Alkhamis, K., Alsoliemy, A., Aljohani, M. M., Alrefaei, A. F., Abumelh, H. M., Mahmoud, M, H. H., Zaky, R., & El-Metwaly, N. (2021). Journal of Molecular Liquids, 340, 117167. https://doi.org/10.1016/j.molliq.2021.117167 CR - Alshehri, S. M., & Ahamad, T. (2010). Bioinorganic Chemistry and Applications, https://doi.org/10.1155/2010/976901 CR - Bakare, S. B. (2019). Polish Journal of Chemical Technology, 21(3), 26-34. https://doi.org/10.2478/pjct-2019-0026 CR - Bray F., Ferlay J., Soerjomataram I., Siegel R.L., Torre, L.A., & Jemal, A. (2018). CA: A Cancer Journal for Clinicians, 68(6), 394-424. https://doi.org/10.3322/caac.21492 CR - Butera, V., D’Anna, L., Rubino, S., Bonsignore, R., Spinello, A., Terenzi, A., & Barone, G. (2023). Journal of Physical Chemistry A, 127(44), 9283–9290. https://doi.org/10.1021/acs.jpca.3c05653 CR - Chai, L. Q., An, H. L., Chen, T. T., & Cai, Y. Y. (2024). Journal of Molecular Structure, 1299, 137151. https://doi.org/10.1016/j.molstruc.2023.137151 CR - Dekker E., Tanis, P.J., Vleugels, J..LA., Kasi, P.M., & Wallace, M.B. (2019). Colorectal cancer. Lancet, 19(10207)1467-1480. https://doi.org/10.1016/S0140-6736(19)32319-0 CR - Demir, I., Diler, S. B., & S. Y. Uçan. (2020). Russian Journal of General Chemistry,90(6), 1051–1054. https://doi.org/10.1134/S1070363220060183 CR - Dumur, F., Contal E., Wantz G., & Gigmes D. (2014). European Journal of Inorganic Chemistry, 2014(25), 4186-4198. https://doi.org/10.1002/ejic.201402422 CR - Es-Sounni, B., Nakkabi, A., Bouymajane, A., Elaaraj, I., Bakhouch, M., Filali, F. R., El Yazidi, M., El Moualij, N., & Fahim, M. (2023). Biointerface Research in Applied Chemistry, 13(4), 333. https://doi.org/10.33263/BRIAC134.333 CR - Findik, M., Ucar, A., Bingol, H., Guler, E., & Ozcan E. (2017). Research on Chemical Intermediates, 43, 401–412. https://10.1007/s11164-016-2630-8. CR - Genomics of Drug Sensitivity in Cancer. (t.y.). Search by drug, gene or cell line name. https://www.cancerrxgene.org CR - González-Hernández, Arturo., León-Negrete, A., Galván-Hidalgo, J., Gómez, E., Villamil-Ramos, R., & Barba, V., (2021). Journal of Molecular Structure, 1242, 130807. https://doi.org/10.1016/j.molstruc.2021.130807 CR - Goyle, S., & Maraveyas A. (2005). Dig Surg, 22(6), 401-414. https://doi.org/10.1159/000091441 CR - He, J., Pei, L., Jiang, H., Yang, W., Chen, J., & Liang H. (2017). Journal of Cancer, 8(7), 1187-1196. https://doi.org/10.7150/jca.18171 CR - Karabulut, S., Karabulut, M., & Tastekin, D. (2021). Journal of Istanbul Faculty of Medicine, 84(3), 425-429. https://doi.org/10.26650/IUITFD.2020.0086 CR - Kumar, V., Robbins, S., & Cotran, R., (Çeviri: U. Çevikbaş). (2010). Temel Patoloji. İstanbul: Nobel Tıp Kitabevleri, (505-514). CR - Mishra, D. K., Singha, U. K., Das, A., Dutta, S., Kar, P., Chakraborty, A., Sen, A., & Sinha, B. (2018). Journal of Coordination Chemistry, 71(14), 1-24. https://doi.org/10.1080/00958972.2018.1476687 CR - Parvarinezhad, S., Ramezanipoor, S., Kubicki, M., Salehi, M. (2024). Applied Organometallic Chemistry, 38, 7477. https://doi.org/10.1002/aoc.7477 CR - Prenen, H., Vecchione, L., & Cutsem V. E. (2013). Targeted Oncology, 8(2), 83-96. https://doi.org/10.1007/s11523-013-0281-x CR - Saha, S., Sasmal, A., Roy Choudhury, C., Gómez-Garcia, C. J., Garribba, E., & Mitra, S. (2014). Polyhedron, 69, 262–269. https://doi.org/10.1016/j.poly.2013.12.006 CR - Samy, F., & Shebl, M. (2022). Applied Organometallic Chemistry, 36(5),6650. https://doi.org/10.1002/aoc.6650 CR - Sharma, V., Yadav, M., Bhatia, A., Muthaiah, S., & Kapoor, J. K. (2024). Journal of Molecular Structure, 1297(2), 136924. https://doi.org/10.1016/j.molstruc.2023.136924 CR - Shekaari, H., Bezaatpour, A., & Elhami-Kalvanagh, R. (2012). Journal of Chemical Thermodynamics, 51, 114–119. https://doi.org/10.1016/j.jct.2012.02.020 CR - Sreejith, S.S., Mohan, N., & Prathapachandra Kurup, M.R. (2017). Polyhedron, 135, 278–295. http://dx.doi.org/10.1016/j.poly.2017.07.015 CR - Srinivas, M., Vijayakumar, G.R., Mahadevan, K.M., Nagabhushana, H., & Bhojya Naik, H.S. (2017). Journal of Science: Advanced Materials and Devices, 2, 156-164. http://dx.doi.org/10.1016/j.jsamd.2017.02.008 CR - Sung, H., Ferlay, J., Siegel, R.L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). CA: A Cancer Journal for Clinicians, 71(3), 209-249. https://doi.org/10.3322/caac.21660 CR - Thakurta, S., Maiti, M., Rosair, G. M., & Tsaturyan, A. A., (2022). Journal of Structural Chemistry, 63(1), 9-18. https://doi.org/10.1134/S0022476622010024 CR - Tomczyk, D., Nowak, L., Bukowski, W., Bester, K., Urbaniak, P., Andrijewski, G., & Olejniczak, B. (2014). Electrochimica Acta, 121, 64–77. https://doi.org/10.1016/j.electacta.2013.12.07 CR - Zakaria, K., Negm, N. A., Khamis, E. A., & Badr, E. A. (2016). Journal of the Taiwan Institute of Chemical Engineers, 61, 316–326. https://doi.org/10.1016/j.jtice.2015.12.021 CR - Zeynali-Moghaddam, S., Mohammadian, M., Kheradmand, F., Fathi-Azarbayjani, A., Rasmi, Y., Esna-Ashari, O., & Malekinejad. H. (2019). Gene, 5(684), 30-38. https://doi.org/10.1016/j.gene.2018.10.016 CR - Zhang, Wei-Guang. (2023). Acta Chimica Slovenica, 70, 421–429. https://doi.org/10.17344/acsi.2023.8144 UR - https://doi.org/10.17714/gumusfenbil.1486484 L1 - https://dergipark.org.tr/tr/download/article-file/3940533 ER -