@article{article_1506379, title={Developing Resistant/Tolerant Charleston Pepper Varieties to Tomato Spotted Wilt Virus (TSWV) Disease Using Existing Pepper Lines}, journal={Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi}, volume={4}, pages={12–20}, year={2024}, author={Basım, Hüseyin and Kandil, Osman and Ökem, Cansu}, keywords={Domates Benekli Solgunluk Virüsü (TSWV), Çarliston biber, MAS}, abstract={Pepper is a vegetable that belongs to the Capsicum genus, which includes 25 species and is part of the Solanaceae family. Several abiotic and biotic stress factors can limit pepper yield in cultivation. One of the top ten viruses that limit pepper production worldwide and cause economic losses is tomato spotted wilt virus (TSWV). The use of chemical control along with cultural practices against viral diseases may sometimes not be effective in controlling the disease. However, the use of resistant varieties is considered the most effective and eco-friendly method of control in the cultivation of peppers. In this study, PCR reactions were conducted using SCAC568 primers to evaluate the resistance/tolerance or susceptibility of 27 different hybrid varieties of Charleston peppers grown under cover, as determined by our breeding program. The PCR products obtained were genotypically analyzed after being digested with XbaI. Based on the results, it was determined that out of the 27 Charleston pepper varieties studied, 66.6% were tolerant and heterozygous (Rr), while 33.4% were susceptible. This study aims to develop TSWV-resistant Charleston pepper varieties.}, number={2}, publisher={Bingöl Üniversitesi}, organization={İstanbul Tarım A.Ş.}