TY  - JOUR
T1  - A new protocol for the induction of chronic mastitis with intramammary infusion of lipopolysaccharide (LPS) in Balb/c mice
TT  - Balb/c farelerinde meme içi lipopolisakkarid uygulaması sonucu yeni bir kronik mastitis modelin indüklenmesi
AU  - Akkoç, Ahmet
AU  - Yavaş, Özkan
PY  - 2024
DA  - December
Y2  - 2024
DO  - 10.31196/huvfd.1530358
JF  - Harran University Journal of the Faculty of Veterinary Medicine
JO  - Harran Univ Vet Fak Derg
PB  - Harran Üniversitesi
WT  - DergiPark
SN  - 2146-7188
SP  - 113
EP  - 117
VL  - 13
IS  - 2
LA  - en
AB  - Mastitis is inflammation of the mammary tissue and is commonly observed in farm animals. The problem causes severe financial losses in the dairy industry in terms of veterinary costs, milk disposal, and treatment expenses. Bacteria are the main actors in the etiology and cause acute and chronic inflammatory changes in the mammary tissue. Acute inflammatory changes are easily recognized clinically, and treatment is initiated immediately, but subacute inflammation progresses insidiously and leads to chronic inflammation with irreversible fibrotic changes. Standardized experimental models for the induction of acute mastitis in laboratory animals are available. Usually, infusion of bacteria or some bacterial structural components into mammary tissue is easily applied for this purpose. However, there are few studies on the induction of chronic mastitis with fibrotic changes, and the applications are relatively complex. In this study, LPS was infused through the teat duct three times on days 0, 5, and 10 to induce chronic mastitis in mice. Tissues were sampled on days 1, 6, and 15 to evaluate histopathological changes. While severe neutrophil infiltrates, a component of acute inflammation, were observed on day 1, lymphocyte infiltrates increased on day 6, consistent with subacute inflammation. On day 15, lesions representing chronic mastitis, such as fibrosis and lymphocyte infiltration, were observed. A model similar to the lesions in chronic mastitis of dairy cattle was successfully and easily established by LPS infusion in mice.
KW  - Balb/c
KW  - Chronic mastitis model
KW  - Lipopolysaccharide
KW  - Mammary
gland
KW  - Masson’s Trichrome
N2  - Mastitis, meme dokusunun iltihaplanmasıdır ve dünya genelinde çiftlik hayvanlarında yaygın olarak görülmektedir. Bu sorun süt endüstrisinde veteriner masrafları, sütün imhası ve tedavi giderleri açısından ciddi mali kayıplara neden olmaktadır. Bakteriler etiyolojinin ana aktörleridir ve meme dokusunda akut ve kronik inflamatuar değişikliklere neden olurlar. Akut enflamatuar değişiklikler klinik olarak kolayca tanınır ve tedavi hemen başlatılır, ancak subakut inflamasyon sinsice ilerler ve geri dönüşü olmayan fibrotik değişikliklerle kronik inflamasyona yol açar. Laboratuvar hayvanlarında akut mastitis indüksiyonu için standartlaştırılmış deneysel modeller mevcuttur ve genellikle bakterilerin veya bazı bakteriyel yapısal bileşenlerin meme dokusuna infüzyonu bu amaçla kolayca uygulanmaktadır. Bununla birlikte, fibrotik değişikliklerle birlikte kronik mastitis indüksiyonu üzerine az sayıda çalışma vardır ve uygulamalar nispeten karmaşıktır. Sunulan çalışmada, farelerde kronik mastitisi indüklemek için LPS 0, 5 ve 10. günlerde üç kez meme kanalından infüze edilmiştir. Histopatolojik değişiklikleri değerlendirmek için 1, 6 ve 15. günlerde dokulardan örnek alınmıştır. Akut inflamasyonun bir bileşeni olan şiddetli nötrofil infiltratları 1. günde gözlenirken, lenfosit infiltratları subakut inflamasyonla uyumlu olarak 6. günde artmıştır. 15. günde, fibrozis ve lenfosit infiltrasyonu gibi kronik mastitisi temsil eden lezyonlar gözlenmiştir. Süt sığırlarının kronik mastitisindeki lezyonlara benzer bir model, farelerde LPS infüzyonu ile başarılı ve kolay bir şekilde oluşturulmuştur.
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UR  - https://doi.org/10.31196/huvfd.1530358
L1  - https://dergipark.org.tr/tr/download/article-file/4132200
ER  -