TY - JOUR T1 - Production of Microspore-Derived Plants by Anther Culture of Cyclamen coum AU - Al-khafaji, Mahdi AU - Yıldız, Mehtap AU - Koçak, Metin AU - Tütüncü, Mehmet PY - 2024 DA - November Y2 - 2024 DO - 10.47115/bsagriculture.1554595 JF - Black Sea Journal of Agriculture JO - BSJ Agri PB - Karyay Karadeniz Yayımcılık Ve Organizasyon Ticaret Limited Şirketi WT - DergiPark SN - 2618-6578 SP - 720 EP - 728 VL - 7 IS - 6 LA - en AB - Cyclamen is one of the most important ornamental crops sold worldwide as a potted flower for winter production. Cyclamen species take up a wide swathe of habitats across Türkiye. Ten wild Cyclamen species grow naturally in Türkiye and some of them are endemic. This study aimed to produce haploid plants of C. coum using anther culture. The microspore developmental stage was evaluated by staining anther with acetocarmine (%2), and then the stage was correlated with bud size. It was determined that the buds between 7.64 and 8.23 mm had the appropriate bud size for the late uninuclear stage. Anthers were cultured in B5 medium containing different levels of 1-Naphthaleneacetic acid (NAA) (0.1, 1, 2 mgL-1), 2,4-Dichlorophenoxyacetic acid (2,4-D) (0.1, 1, 2 mgL-1), and kinetin (0, 1 mgL-1), 90 gL-1 sucrose and 3 gL-1 gelrite for haploid embryo production. Anthers were kept at 4°C for 4 days after culture. The explants were incubated at 24°C in a completely dark condition until the embryo was formed, then embryos were transferred to hormone-free media in 16:8 hours (light (75 µmolm-2s-1): dark) photoperiod. The experiment was carried out for two years. In the first year, 12 different media were examined in view of regeneration and the experiments were continued with selected 7 media in the second year. 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