TY  - JOUR
T1  - Exosome isolation by ultracentrifugation and precipitation methods from human adipose-derived mesenchymal stem cells and the effects on human colon cancer cell line.
TT  - İnsan yağ dokusundan elde edilen mezenkimal kök hücrelerinden ultra santrifüj ve çöktürme yöntemleriyle ekzosom izolasyonu ve insan kolon kanseri hücre hattına etkileri.
AU  - Yıldırım, Hatice
AU  - Efe Daşkan, Burcu
AU  - Köçkar, Feray
PY  - 2025
DA  - April
Y2  - 2024
DO  - 10.38001/ijlsb.1571132
JF  - International Journal of Life Sciences and Biotechnology
JO  - Int J. Life Sci. Biotechnol.
PB  - International Society of Academicians
WT  - DergiPark
SN  - 2651-4621
SP  - 38
EP  - 44
VL  - 8
IS  - 1
LA  - en
AB  - Exosomes, one of the extracellular vesicles, mediate molecular information and intercellular communication between cells by containing various proteins, lipids, mRNA, metabolic enzymes and numerous essential molecules such as miRNAs. The therapeutic potential of paracrine factors of exosomes released from human adipose mesenchymal stem cells is still under investigation. In our study, we isolated exosomes released from human adipose mesenchymal stem cells by two different methods. To evaluate the cytotoxic effect of the obtained exosomes, we performed MTT assay in SW-480 cell line at 24, 48 and 72 hours. In the results obtained, no statistically significant difference was observed at 24 and 72 hours, while statistically significant differences were determined between the concentration groups at 48 hours.
KW  - MTT
KW  - exosome
KW  - colon cancer
KW  - mesenchymal stem cells
N2  - Hücre dışı veziküllerden biri olan ekzosomlar, çeşitli proteinler, lipitler, mRNA, metabolik enzimler ve miRNA gibi çok sayıda esansiyel molekülü içererek hücreler arasındaki moleküler bilgiyi ve hücreler arası iletişimi sağlar. İnsan yağ mezenkimal kök hücrelerinden salınan ekzosomların parakrin faktörlerinin terapötik potansiyeli hala araştırılmaktadır. Çalışmamızda, insan yağ mezenkimal kök hücrelerinden salınan ekzosomlar iki farklı yöntemle izole edildi. Elde edilen ekzosomların sitotoksik etkisini değerlendirmek için SW-480 hücre hattında 24, 48 ve 72. saatlerde MTT analizi yapıldı. Elde edilen sonuçlarda, 24 ve 72. saatlerde istatistiksel olarak anlamlı bir fark gözlenmezken, 48. saatte konsantrasyon grupları arasında istatistiksel olarak anlamlı farklar belirlendi.
CR  - Théry, C., Laurence Z., and Sebastian A., Exosomes: composition, biogenesis and function. Nature reviews immunology, 2002. 2.8: p. 569-579.
CR  - Yang, D., Weihong Z., Huanyun Z., Fengqiu Z., Lanmei C., Lixia M., Leon M L., Suxiang C., Nan L., Qingxia Z., Phuong HL T., Changying C., Rakesh N V., Tao W., Progress, opportunity, and perspective on exosome isolation-efforts for efficient exosome-based theranostics. Theranostics, 2020. 10.8: 3684.
CR  - Mikkel Ø. N., Lasse B. S., Didde R. H., Ernst-Martin F., Morten B. E., Henrik D., Ditte C. A., Per S., A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles. Scientific Reports, 2022. 12.1: 496.
CR  - Farooqi, A. A., et al. Exosome biogenesis, bioactivities and functions as new delivery systems of natural compounds. Biotechnology advances, 2018. 36.1: p. 328-334.
CR  - Bobrie, A., et al. Exosome secretion: molecular mechanisms and roles in immune responses. Traffic, 2011. 12.12: p. 1659-1668.
CR  - Dicker, A., et al. Functional studies of mesenchymal stem cells derived from adult human adipose tissue. Experimental cell research, 2005. 308.2: p. 283-290.
CR  - Yang, X. F., et al. High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells. Journal of biomedical science, 2011, 18: p. 1-9.
CR  - Heo, J. S., Youjeong C., and Hyun O.  K., Adipose‐derived mesenchymal stem cells promote M2 macrophage phenotype through exosomes. Stem cells international, 2019.  2019.1: p. 792-1760.
CR  - Fang, Y., et al. Adipose-derived mesenchymal stem cell exosomes: a novel pathway for tissues repair. Cell and Tissue Banking, 2019. 20: p. 153-161.
CR  - Xing, X., et al. Proteomic analysis of exosomes from adipose‐derived mesenchymal stem cells: a novel therapeutic strategy for tissue injury. BioMed research international, 2020. 2020.1: p. 609-4562.
UR  - https://doi.org/10.38001/ijlsb.1571132
L1  - http://dergipark.org.tr/tr/download/article-file/4304299
ER  -