TY - JOUR T1 - Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein TT - Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein AU - Bayyurt, Burcu AU - Arslan, Serdal PY - 2025 DA - January Y2 - 2024 DO - 10.34248/bsengineering.1578992 JF - Black Sea Journal of Engineering and Science JO - BSJ Eng. Sci. PB - Karyay Karadeniz Yayımcılık Ve Organizasyon Ticaret Limited Şirketi WT - DergiPark SN - 2619-8991 SP - 54 EP - 57 VL - 8 IS - 1 LA - en AB - Oxidized low-density lipoprotein (ox-LDL) shows many harmful effects such as induction of apoptosis on function of endothelial cell (EC). Fas ligand (FASL) induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells (HUVECs) with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction (qPCR). We compared FASL gene expression level between the groups according to ∆∆Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL (P KW - Oxidized low-density lipoprotein KW - Fas ligand KW - Gene expression KW - Quantitative polymerase chain reaction KW - Human umbilical vein endothelial cell N2 - Oxidized low-density lipoprotein (ox-LDL) shows many harmful effects such as induction of apoptosis on function of endothelial cell (EC). Fas ligand (FASL) induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells (HUVECs) with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction (qPCR). We compared FASL gene expression level between the groups according to ∆∆Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL (P CR - Cao X, Yang J, He L, Liu C. 2024. Circ_0005699 Expedites ox-LDL-Triggered endothelial cell injury via targeting miR-384/ASPH axis. Cardiovasc Toxicol, 24 (10): 1067-1076. CR - Imanishi T, Hano T, Sawamura T, Takarada S, Nishio I. 2002. Oxidized low density lipoprotein potentiation of Fas-induced apoptosis through lectin-like oxidized-low density lipoprotein receptor-1 in human umbilical vascular endothelial cells. Circ J, 66 (11): 1060-1064. CR - Khatana C, Saini NK, Chakrabarti S, Saini V, Sharma A, Saini RV, Saini AK. 2020. 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New insights into the role of inflammation in the pathogenesis of atherosclerosis. Int J Mol Sci, 18 (10): 2034. UR - https://doi.org/10.34248/bsengineering.1578992 L1 - https://dergipark.org.tr/tr/download/article-file/4338496 ER -